Poly a t

Manufactured by Merck Group

Sourced in United States

Poly A:T is a laboratory equipment product designed for various molecular biology applications. It functions as a nucleic acid preparation tool, specifically for the purification and isolation of polyadenylated RNA from biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Nigericin, by Merck Group (6 mentions) Mitosox, by Thermo Fisher Scientific (6 mentions) Adenosine triphosphate (atp), by Merck Group (5 mentions) Mitotracker, by Thermo Fisher Scientific (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Ultrapure lps, by Thermo Fisher Scientific (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Mitotracker red, by Thermo Fisher Scientific (2 mentions) Pam3csk4, by Merck Group (2 mentions) Phorbol myristate acetate (pma), by Merck Group (2 mentions) Mcc950, by Selleck Chemicals (2 mentions) Ultrapure lps, by InvivoGen (2 mentions) Protein g agarose, by Merck Group (2 mentions) Anti human caspase 1, by Cell Signaling Technology (2 mentions) Glucose, by Merck Group (2 mentions) Mntbap, by Santa Cruz Biotechnology (2 mentions) Anti mouse caspase 1 p20 ag 20b 0042, by Adipogen (2 mentions) Anti nek7 sc 50756, by Santa Cruz Biotechnology (2 mentions) Anti nlrp3 ag 20b 0014, by Adipogen (2 mentions) Anti mouse nlrp3 ag 20b 0014, by Adipogen (2 mentions)

7 protocols using poly a t

Isolation and Characterization of Inflammasome Activation

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Kynurenic acid (K3375, purity≥98%) was obtained from Sigma−Aldrich company (Missouri, USA). M-CSF was obtained from NOVUS (Colorado, USA). LPS, nigericin, ATP, MSU, Pam3CSK4, PMA, and poly(A/T) were obtained from Sigma−Aldrich (Missouri, USA). MitoTracker Red, MitoSOX and Lipofectamine 2000 were obtained from Invitrogen (California, USA). Flou-4 AM and DAPI were obtained from Beyotime (Shanghai, China). The Salmonella typhimurium strain ATCC-14028 was obtained from Beina Chuanglian Biotech Co., Ltd. (Beijing, China).

Sun T., Xie R., He H., Xie Q., Zhao X., Kang G., Cheng C., Yin W., Cong J., Li J, & Wang X. (2022). Kynurenic acid ameliorates NLRP3 inflammasome activation by blocking calcium mobilization via GPR35. Frontiers in Immunology, 13, 1019365.

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Mapping STING-Mediated Signaling Cascades

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Poly(I:C) and Poly(A:T) [dAdT] was purchased from Sigma Aldrich. c-di GMP, c-di AMP, 2′3′ cGAMP, 3′3′ cGAMP, and DMXAA was purchased from Invivogen. Lipofectamine 2000 (Life Technologies) was used to transfect c-di GMP, c-di AMP, 2′3′ cGAMP, 3′3′ cGAMP, and dAdT. Xtremegene 9 to transfect Poly(I:C). For stimulation experiments macrophages were plated at a density of 10⁶ cells/mL. C-di-nucleotide stimulations done as indicated at 2ug or 4ug/mL. Antibodies: (Cell Signaling): STING #3337; p65 #8242; Phospho-p65 #3033; ERK #4695; Phospho -ERK #4370; p38 #9212; Phospho -p38 #4511; TBK1 #3013; Phospho -TBK1 #5483; Phospho -IRF3 #4947; JNK #9258; Phospho -JNK #9251; HA-Tag #3724.(ENZO): Grp94, #ADI-SPA-850.

Surpris G., Chan J., Thompson M., Ilyukha V., Liu B.C., Atianand M., Sharma S., Volkova T., Smirnova I., Fitzgerald K.A, & Poltorak A. (2015). Novel Tmem173 allele reveals importance of STING N-terminus in trafficking and type I IFN production. Journal of immunology (Baltimore, Md. : 1950), 196(2), 547-552.

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Inflammasome Activation Pathway Analysis

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MSU, Nigericin, ATP, PMA (phorbol‐12‐myristate‐13‐acetate), poly A:T, insulin, and glucose were purchased from Sigma. TR and MCC950 were obtained from Selleck. The ultrapure LPS and Pam3CSK4 (tripalmitoylcysteinylseryltetralysinelipopeptide) were from Invivogen. Imject‐Alum was from Pierce Biochemicals. MitoTracker and MitoSOX were from Invitrogen. Protein G agarose was from Millipore. Anti‐β‐actin (1:5,000, P30002) and Anti‐DYKDDDDK‐Tag mAb were from Abmart. Anti‐human pro‐IL‐1β (1:1,000, 60136‐1‐Ig), anti‐TRPV2 (1:1,000, 15991‐1‐AP), and anti‐HPGDS (1:1,000, 22522‐1‐AP) were from Proteintech. Anti‐mouse IL‐1β (1:1,000, AF‐401‐NA) was from R&D Systems. Anti‐mouse caspase‐1 (p20) (1:1,000, AG‐20B‐0042) and anti‐NLRP3 (1:1,000, AG‐20B‐0014) were from Adipogen. Anti‐human caspase‐1(1:1,000, 2225) was from Cell Signaling. Anti‐ASC (1:500, sc‐22514‐R) and anti‐NEK7 (1:500, SC‐50756) were from Santa Cruz. Anti‐human cleaved IL‐1β (1:1,000, A5208206) was from Sangon Biotech. Anti‐Flag (1:2,000, F2555) or anti‐VSV (1:2,000, V4888) was from Sigma. Recombinant human NLRP3 was from Novus Biologicals. Salmonella is a gift from R.V. Bruggen.

Huang Y., Jiang H., Chen Y., Wang X., Yang Y., Tao J., Deng X., Liang G., Zhang H., Jiang W, & Zhou R. (2018). Tranilast directly targets NLRP3 to treat inflammasome‐driven diseases. EMBO Molecular Medicine, 10(4), e8689.

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Cytokine Activation Pathway Analysis

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Phorbol-12-myristate-13-acetate (PMA), MSU, ATP, nigericin, poly A:T, Pam3CSK4, A9C, DIDS, and IAA94 were purchased from Sigma. MitoSox, MitoTracker Red, DAPI, MQAE, Lipofectamine RNAiMAX, Lipofectamine 2000, and M-MLV were from Invitrogen. Ultrapure LPS was obtained from Invivogen. SYRB Green was from TransGen Biotech. MnTBAP was from Santa Cruz. Salmonella is a gift from R.V. Bruggen. Anti-human cleaved IL-1β (2021, 1:1000 dilution) and anti-human caspase-1(2225, 1:1000 dilution) antibodies were purchased from Cell Signalling. Anti-human pro-IL-1β (60136-1-lg, 1:1000 dilution) anti-CLIC1(14545-1-AP, 1:500 dilution), anti-GAPDH (10494-1-AP, 1:1000 dilution), anti-VDAC1 (66345-1-lg, 1:1000 dilution), and anti-ATP1A1 (55187-1-AP, 1:1000 dilution) antibodies were from Proteintech. Anti-CLIC4 (SC-135739, 1:1000 dilution), anti-NEK7 (SC-50756, 1:500 dilution), and anti-ASC (rabbit, SC-22514, 1:400 dilution) antibodies were from Santa Cruz. Anti-ASC (mouse, 04–147, 1:1000 dilution) antibody was from Merck Millipore. Anti-CLIC5 (ACL-025, 1:250 dilution) antibody was from Alomone labs. Anti-mouse IL-1β (AF-401-NA, 1:1000 dilution) antibody was from R&D. Anti-mouse caspase-1 (p20) (AG-20B-0042, 1:1000 dilution) and anti-NLRP3 (AG-20B-0014, 1:1000 dilution) antibodies were from Adipogen. Anti-β-actin (P30002, 1:1000 dilution) antibody was from Abmart.

Tang T., Lang X., Xu C., Wang X., Gong T., Yang Y., Cui J., Bai L., Wang J., Jiang W, & Zhou R. (2017). CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. Nature Communications, 8, 202.

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NLRP3 Inflammasome Activation Assay

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Apilimod (No. S6414) was bought from Selleck. Nigericin, MSU, and poly (A/T) were purchased from Sigma-Aldrich. Ultrapure LPS, MitoSOX, MitoTracker, DAPI, Lysotracker Green, MitoTracker Deep Red, MitoTracker Green were acquired from Invitrogen. MnTBAP was bought from Santa Cruz Biotechnology. The C3 toxin was a gift from Dr. Tengchuan Jin (University of Science and Technology of China, Hefei, China). CY-09 was synthesized from Dr. Xianming Deng (Xiamen University, Xiamen, China). MCC950 was bought from Selleck. Fluo-4, AM(HY-101896), ML-SI3 were purchased from MedChemExpress. BAPTA-AM(A1076) was bought from Sigma-Aldrich. Protein G agarose (16–266) was bought from Millipore. Anti-Flag (F2555) antibodies were from Sigma-Aldrich. The anti-mouse NLRP3 (AG-20B-0014) and the anti-mouse caspase-1 (AG-20B-0042) antibodies was from AdipoGen. The anti-mouse IL-1β antibody (AF-401-NA) was obtained from R&D Systems. The anti-β-actin antibody (66009-1-Ig) was from Proteintech Group. The anti-mouse ASC antibody (67824S) was from Cell Signaling Technology. The anti-mouse NEK7 antibody (ab133514) was from Abcam.

Hou Y., He H., Ma M, & Zhou R. (2023). Apilimod activates the NLRP3 inflammasome through lysosome-mediated mitochondrial damage. Frontiers in Immunology, 14, 1128700.

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NLRP3 Inflammasome Activation Assay

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ATP, Nigericin, MSU, and poly (A/T) were purchased from Sigma-Aldrich. Ultrapure LPS, Lipofextamine 2000, Pam3CSK4, MQAE, MitoSOX, MitoTracker, DAPI were acquired from Invitrogen. The Salmonella strain was a gift from Dr. Cai Zhang (Shandong University, Shandong, China). The C3 toxin was a gift from Dr. Tengchuan Jin (University of Science and Technology of China, Hefei, China). CY-09 was synthesized from Dr. Xianming Deng (Xiamen University, Xiamen, China). Protein G agarose (16-266) and anti-Flag (A2220) beads were bought from Millipore and Sigma-Aldrich, respectively. Anti-Flag (F2555) and anti-VSV (V4888) antibodies were from Sigma-Aldrich. The anti-mouse NLRP3 (AG-20B-0014) and the anti-mouse caspase-1 (AG-20B-0042) antibodies was from AdipoGen. The anti-mouse IL-1β antibody (AF-401-NA) was obtained from R&D Systems. The anti-β-actin antibody (66009-1-Ig) was from Proteintech Group. The anti-mouse ASC antibody (67824S) was from Cell Signaling Technology. The anti-mouse NEK7 antibody (ab133514) was from Abcam.

Lin H., Yang M., Li C., Lin B., Deng X., He H, & Zhou R. (2022). An RRx-001 Analogue With Potent Anti-NLRP3 Inflammasome Activity but Without High-Energy Nitro Functional Groups. Frontiers in Pharmacology, 13, 822833.

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NLRP3 Inflammasome Activation and IL-1β Regulation

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ATP, MSU, poly A:T, nigericin, and glucose were from Sigma. The human recombinant insulin was from Nova Nordisk. One Touch^® Ultra^® Blood glucose Test System was from Roche. Oridonin (s2335) was bought from Selleck. MitoSOX, ultrapure LPS, Pam3CSK4 and MitoTracker were from Invitrogen. Protein G agarose and streptavidin-coated beads were, respectively, supplied by Millipore and Pierce Biochemicals. Anti-Flag (F2555) and anti-VSV (V4888) were bought from Sigma. Anti-NLRP3 (AG-20B-0014) and anti-mouse caspase-1 (p20) (AG-20B-0042) antibodies were from Adipogen. Anti-NEK7 (SC-50756) and anti-ASC (sc-22514-R) antibodies were obtained from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17) (AF-401-NA) was from R&D Systems. A standard high-fat diet (D12492, 60% kcal fat) was from Research Diet Company.

He H., Jiang H., Chen Y., Ye J., Wang A., Wang C., Liu Q., Liang G., Deng X., Jiang W, & Zhou R. (2018). Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity. Nature Communications, 9, 2550.

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