The crystal structures and element purity of the samples were characterized using an X-ray diffractometer, XRD (Bruker AXS D8 Advance). The morphology evolution of each sample was investigated via a field emission scanning electron microscopy, FESEM (Zeiss Merlin Gemini 2) whereas the effective thickness of the samples were measured with a surface profilometer (Veeco Deetak M6). Additionally, the chemical composition and binding energy of the samples were examined by an X-ray photoelectron spectroscopy, (XPS Microprobe, PHI Quantera II), using Al Kα as a monochromatic radiation source at room temperature. The optical properties of the samples were studied by a UV-vis-NIR spectrometer (Perkin Elmer Lambda 900) and a steady-state photoluminescence (PL) spectrophotometer (Edinburgh Instruments FLS920) at an excitation wavelength of 300 and 472 nm, respectively. The time-resolved PL (TRPL) spectrum and exciton decay lifetime of x-FZNR/P3HT active layers were obtained using a 472.4 nm picosecond laser diode as the excitation source using the same PL set-up.
Merlin gemini 2
Manufactured by Zeiss
Sourced in Germany
The Merlin Gemini II is a high-performance scanning electron microscope (SEM) developed by Zeiss. It is designed to provide advanced imaging and analytical capabilities for a wide range of applications. The Merlin Gemini II utilizes a field emission gun (FEG) electron source and Zeiss' proprietary optics to deliver high-resolution images and precise surface analysis.
Automatically generated - may contain errors
Lab products found in correlation
Q150rs, by Quorum Technologies (10 mentions)
Autosamdri 931, by Tousimis (2 mentions)
Glutaraldehyde, by Polysciences (2 mentions)
Axs d8 advance, by Bruker (1 mentions)
Lambda 900, by PerkinElmer (1 mentions)
Fls920, by Edinburgh Instruments (1 mentions)
Jem 2100, by JEOL (1 mentions)
D8 advance, by Bruker (1 mentions)
Originpro, by OriginLab (1 mentions)
Tm3000, by Hitachi (1 mentions)
Smartsem, by Zeiss (1 mentions)
Imagej, by Adobe (1 mentions)
Eos 700d, by Canon (1 mentions)
Spring 5uv purification system, by Hydrolab (1 mentions)
Ols4000, by Olympus (1 mentions)
Quorum q150r s, by Quorum Technologies (1 mentions)
Tecnai osiris, by Thermo Fisher Scientific (1 mentions)
X pert x ray diffractometer, by Philips (1 mentions)
18 protocols using merlin gemini 2
1
Comprehensive Characterization of Nano-Structured Materials
2
Composite Microstructure Analysis by SEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To characterize the morphology, evaluate the state of adhesion/dispersion of the fibers in the matrix and the effects of water absorption on the microstructure of composites SEM observations of flexural fracture surfaces of specimens before aging and re-dried aged specimens, were performed using a Merlin Gemini 2 instrument (Zeiss) The fractures portions of the specimens were cut and sputter-coated with a fine layer of gold over the surface uniformly for examination.
3
Characterization of Ceramic Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The X-ray diffraction (XRD) (Bruker D8 Advance) was employed to investigate the information regarding phase and structure features of all samples. The XRD patterns of all samples were recorded within the range of 20° to 120° with a step size of 0.019° and a scan rate of 0.064°/min. Furthermore, High-resolution transmission electron microscopy (HRTEM) (JEOL-JEM-2100) was employed to study the particle size, particle morphology, lattice plane, etc. of the powder calcined at 1100 °C for 4 h. The microstructure features (i.e. grain size, grain morphology, etc.) were investigated through Field emission scanning electron microscopy (FESEM) of Zeiss (Merlin-Gemini II). The distributions of grain size were estimated through ImageJ software for all the samples. The Raman spectroscopic measurement of all sintered samples at 1600 °C was carried out using double pre-monochromator (1800 grooves/mm grating) of Raman spectrophotometer (T64000, Horiba Jobin Yvon Ltd. USA) with 514.5 nm radiation of Ar+ laser.
4
Nanofiber Morphology Analysis via SEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A scanning electron microscope (SEM, Merlin Gemini II, ZEISS, Germany) was used to analyze nanofiber and film morphology. Prior to imaging, samples were coated with a 10 nm gold layer using a rotary pump sputter coater (Q150RS, Quorum Technologies, UK). The SEM imaging was performed with an accelerating voltage of 2.5 kV and current of 110 pA at a working distance of 7 mm. ImageJ software (version 1.50i, National Institutes of Health, USA) was utilized to determine nanofiber diameters and calculate fiber (Ff) and pore (Pf) fractions from SEM macrographs. The sum of Ff and pore Pf should reach about 100%. The Ff supports the similar information about the meshes as the commonly used shade coefficient in FWC.5,13 (link) The average nanofiber diameter was calculated from 100 measurements presented in histograms prepared using OriginPro (2018b, OriginLab, USA). The fraction of fiber and pore analysis were performed using the particle function in ImageJ based on the images showed in Fig. 1c and d . The pore size was calculated from SEM binary images prepared using the Li thresholding method in ImageJ.45,46 (link) The threshold was set to 0–90 for Pf and pore size calculation. The Ff was calculated from inverted binary 2D images.
5
Characterization of Electrospun PCL Fibers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were coated with approximately 5 nm Au layer using rotary-pumped sputter coating (Q150RS, Quorum Technologies, Lewes, UK). SEM (Merlin Gemini II, Zeiss, Munich, Germany) was used for imaging, applying a current of 20 pA and voltage of 3 kV. Fiber diameters (Figure 1 ) and sample thickness (see Figure S1 in the Supplementary Materials ) were measured from SEM images using Fiji (Life-Line Version 2.0, Bethesda, MD, USA).
The mechanical properties of PCL fiber mats were measured using a tensile module with 1 N load cell (Kammrath Weiss GmbH, Dortmund, Germany). The tensile module is shown inFigure S2 in the Supplementary Material . The fiber mats were placed within the frames of a 2 mm × 1.7 mm area with cut sides. Mechanical tests were performed uniaxially with an extension speed of 50 μm·s−1. Maximum stress and strain were calculated from stress-strain curves using Origin Integrate Function.
The mechanical properties of PCL fiber mats were measured using a tensile module with 1 N load cell (Kammrath Weiss GmbH, Dortmund, Germany). The tensile module is shown in
6
Triboelectric Yarn Cross-Sectional Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SEM was conducted using a Hitachi TM3000
with an accelerating voltage of 15 kV and a working distance of 3
mm. The triboelectric yarn cross-sectional investigation was performed
using a Zeiss Merlin Gemini II. The sample was freeze-fractured in
liquid nitrogen and imaged with an accelerating voltage of 3 kV and
150 pA current at a working distance of 3–8 mm. The freeze-fractured
samples were gold-coated using a rotary-pump sputter coater (Q150RS,
Quorum Technologies) with a 10 nm layer prior to imaging. The remaining
samples were imaged uncoated. The fiber diameter measurements were
performed utilizing ImageJ v1.5 software.
with an accelerating voltage of 15 kV and a working distance of 3
mm. The triboelectric yarn cross-sectional investigation was performed
using a Zeiss Merlin Gemini II. The sample was freeze-fractured in
liquid nitrogen and imaged with an accelerating voltage of 3 kV and
150 pA current at a working distance of 3–8 mm. The freeze-fractured
samples were gold-coated using a rotary-pump sputter coater (Q150RS,
Quorum Technologies) with a 10 nm layer prior to imaging. The remaining
samples were imaged uncoated. The fiber diameter measurements were
performed utilizing ImageJ v1.5 software.
7
Fiber Morphology Characterization via SEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Small cuts from each sample were mounted on a specimen stub with carbon tape. Then, the pieces were coated with an 8 nm layer of gold using a rotary pump sputter coater (Q150RS, Quorum Technologies, Laughton, UK). The morphology of the samples was studied using SEM (Merlin Gemini II, Zeiss, Oberkochen, Germany) at 2 kV, 100 pA. The working distance was optimized at 8–9 mm, and a secondary electron detector was utilized. The average fiber diameters were measured from 100 fibers based on 4 SEM images per sample using the ImageJ software (v. 1.51j8, Bethesda, MD, USA).
8
Scanning Electron Microscopy of Cellular Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SEM of S.Tm inocula, HeLa cells, m‐ICcl2 cells, and mouse intestine samples was performed as described previously (Fattinger et al., 2020 (link)). Briefly, samples were fixed in 2.5% glutaraldehyde (Polyscience), washed in Krebs‐Ringer buffer or PBS, and treated with 1% OsO4 (Polyscience). HeLa and m‐ICcl2 cells were additionally incubated in 0.5% carbohydrazide and treated with 1% OsO4 for a second time. All samples were incubated in acetone before dehydration and critical‐point‐drying by liquid CO2 using an Autosamdri‐931 (Tousimis or Bal‐Tec CPD030). Samples were mounted on aluminum SEM stubs, sputter‐coated with 5 nm platinum/palladium (Safematic CCU‐010 or Bal‐Tec SCD500). Samples were explored using a Zeiss Merlin Gemini II ultra‐high resolution field emission scanning electron microscope (acceleration voltage 5 kV) and images were captured and analyzed with Zeiss SmartSEM and ImageJ. Where relevant, pseudo‐coloring was applied post‐processing in Adobe Illustrator.
9
Sputter Coating and SEM Analysis of Electrospun Fibers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Prior to imaging with SEM, all samples of electrospun fibers and films were sputter coated with 5 nm gold layer using rotary-pump sputter coater (Q150RS, Quorum Technologies, Laughton, UK). We used an accelerating voltage of 3 kV and a current of 150 pA at a working distance of 6 mm in SEM (Merlin Gemini II, Zeiss, Germany). The average Df was measured on 100 fibers using ImageJ [58 (link)] (v.1.51g) for all electrospun polymers with standard deviations, see histograms and micrographs in Figure 1 SEM micrographs of all spin-coated films are included in Supporting Information , see Figure S1 . From SEM micrographs of electrospun surfaces, uploaded to ImageJ, we calculated Ff. Prior, the images were made binary using DiameterJ plug-in for the best automatic threshold and next from the obtained images, pixels were counted using histogram plug-in. This ratio of white to black pixels indicated the Ff. The representative binary images for Ff analysis are presented in Figure S3 in the Supporting Information .
10
Evaluating Thrombogenic Behavior of CDI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The
slide method was used to calculate the clotting time by observing
the fibrin strand formation time in the capillary blood collected
aseptically. Whole blood without the sample served as control. Assays
for prothrombin time (PT), thrombin time (TT), and activated partial
thromboplastin time (aPTT or APTT) were performed to evaluate the
thrombogenic behavior of the CDI sample by keeping it in whole blood
collected in a citrate tube through central laboratory facilities.
Percentage hemolysis was quantified from a colorimetric assay after
incubating the CDI samples with blood at 37 °C for 3 h, with
gentle shaking every 30 min, followed by pelleting by centrifugation
at the rate of 3000g for 15 min. Hemolysis in distilled
water was taken as the control.38 (link) Whole
blood without the sample was taken as reference for the study. The
international normalized ratio (INR) was calculated for the sample.
Platelet adsorption test was performed for the CDI sample by incubating
the sample in platelet-rich plasma (PRP) diluted with PBS (1:1) for
15 min, followed by rinsing with PBS to eliminate the unadsorbed platelets.
Samples were prepared for SEM (Zeiss Merlin Gemini II) after paraformaldehyde
(3.7%) fixation. A glass coverslip was taken as control.
slide method was used to calculate the clotting time by observing
the fibrin strand formation time in the capillary blood collected
aseptically. Whole blood without the sample served as control. Assays
for prothrombin time (PT), thrombin time (TT), and activated partial
thromboplastin time (aPTT or APTT) were performed to evaluate the
thrombogenic behavior of the CDI sample by keeping it in whole blood
collected in a citrate tube through central laboratory facilities.
Percentage hemolysis was quantified from a colorimetric assay after
incubating the CDI samples with blood at 37 °C for 3 h, with
gentle shaking every 30 min, followed by pelleting by centrifugation
at the rate of 3000g for 15 min. Hemolysis in distilled
water was taken as the control.38 (link) Whole
blood without the sample was taken as reference for the study. The
international normalized ratio (INR) was calculated for the sample.
Platelet adsorption test was performed for the CDI sample by incubating
the sample in platelet-rich plasma (PRP) diluted with PBS (1:1) for
15 min, followed by rinsing with PBS to eliminate the unadsorbed platelets.
Samples were prepared for SEM (Zeiss Merlin Gemini II) after paraformaldehyde
(3.7%) fixation. A glass coverslip was taken as control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!