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Hexadecyltrimethylammonium bromide ctab

Manufactured by Thermo Fisher Scientific
Sourced in Belgium, Spain

Hexadecyltrimethylammonium bromide (CTAB) is a cationic surfactant commonly used in molecular biology and biochemistry applications. It is a white crystalline solid with a chemical formula of CH3(CH2)15N(CH3)3Br. CTAB is primarily used for its ability to disrupt cell membranes and facilitate the extraction and purification of nucleic acids, such as DNA and RNA, from biological samples.

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4 protocols using hexadecyltrimethylammonium bromide ctab

1

Synthesis of Nanomaterials for Environmental Applications

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The following materials were used in the experiments: ethanol (EtOH, 99.7+%, Reanal, Budapest, Hungary), 2-propanol (99+%, Acros Organics, Geel, Belgium), purified distilled water (filtered with Millipore Simplicity 185 system to reach 18.2 MΩ cm), tetraethyl orthosilicate (TEOS, 99%, Merck, Budapest, Hungary), titanium (IV) butoxide (98%, Merck, Budapest, Hungary), titanium (IV) isopropoxide (98+%, Acros Organics, Geel, Belgium), hydrochloric acid (HCl, 37%, Reanal, Budapest, Hungary), nitric acid (HNO3, 65%, Lach-Ner, Bratislava, Slovakia), ammonia aqueous solution (NH3, 25%, Lach-Ner, Bratislava, Slovakia), acetylacetone (99+%, Acros Organics, Geel, Belgium), hexadecyltrimethylammonium bromide (CTAB, 99+%, Acros Organics, Geel, Belgium), Pluronic P123 (P123, average mol wt 40000, Sigma Aldrich, Budapest, Hungary), AgNO3 (99.8%, Lach-Ner, Bratislava, Slovakia), rhodamine 6G (R6G, 95%, Sigma Aldrich, Budapest, Hungary), methylene blue (MB, 95+%, Reanal, Budapest, Hungary) and methyl orange (MO, 95%, Acros Organics, Geel, Belgium) dyes. All materials were analytical grade and used without further purification.
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2

Synthesis of Multilayered Nanomaterials

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Poly(diallyldimethylammonium chloride) (PDDA, Mw < 100,000 (very low molecular weight), 35 wt.% in H2O, positively charged non-biodegradable PEE, ref. 522376), poly-L-lysine-fluorescein isothiocyanate (PLL-FITC) (Mw ~15,000–30,000) labeled, positively charged biodegradable PEE ref. 3543), poly-(sodium 4 styrenesulfonate) (PSS, Mw ~70,000, negatively charged non-biodegradable PEE, ref. 243051), and tetraethyl orthosilicate (TEOS, 98%, ref. 131903) were all purchased from Sigma-Aldrich (Madrid, Spain) and hexadecyltrimethylammonium bromide (CTAB, 99%, ref. 227160100) was purchased from ACROS Organics (The Hague, Nederlands). Multiwalled carbon nanotubes 9.5 nm in diameter and 1.5 μm in length, 95% purity, synthesized using Catalytic Chemical Vapor Deposition process, were purchased from Nanocyl (Sambreville, Belgium) as a powder, and Milli-Q water was prepared in a three-stage Millipore Milli-Q plus 185 purification system with a resistivity higher than 18.2 MΩ cm, pH 5.5.
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3

Synthesis of Metal Oxide Nanoparticles

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Precursor salts of Ni(NO3)2•6H2O (99%), Mg(NO3)2•6H2O (99 + %), Al(NO3)3•9H2O (99 + %), ZrO(NO3)2•H2O (99.5%), Co(NO3)2•6H2O (99 + %), Ga(NO3)3•H2O (99.99%), Ce(NO3)3•6H2O (99.5%), and Cr(NO3)3•9H2O (99%) were purchased from Acros Organics; In(NO3)3•H2O (99.99%), Nd(NO3)3•6H2O (99.9%), Er(NO3)3•5H2O (99.9%), and Pt(NO3)4 solution (Pt 15 w/w) were purchased from Alfa Asear; Fe(NO3)3•9H2O was purchased from Fisher Chemical; Zn(NO3)2•6H2O (98%) was purchased from Sigma Aldrich; Pd(NO3)2•H2O (99.8%, Pd 39% min) and IrCl3•3H2O (Ir 53–56%) were purchased from Thermo Scientific. NaOH pellets were purchased from Sigma Aldrich. Hexadecyltrimethylammonium bromide (CTAB, 99 + %) and HCl (37%) were purchased from Acros Organics. Ethanol (200 proof) was obtained from Decon Labs, Inc.
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4

Functional Bioactive Surfaces Synthesis

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All solvents for synthesis were purchased from VWR and used as received. Water (18.2 MΩ∙cm) was collected from an Arium (Sartorius) laboratory-grade water purification system. Gadolinium(III) nitrate hexahydrate (Gd(NO3)3·6H2O), sodium tungstate (Na2WO4) and potassium chloride (KCl) were purchased from VWR. Hexadecyl-trimethylammonium bromide (CTAB, 99 + %) was purchased from ACROS ORGANICS. 2-methoxy(polyethyleneoxy)propyltrimethoxysilane (90%, 6–9 PEG units) was purchased from ABCR Chemie. Triethylamine (TEA, > 99.5%) and hydrochloric acid (HCl, 32 wt. % in H2O) were purchased from Merck. Sulfo-cyanine3 (Cy3) NHS ester (95%) was purchased from Lumiprobe. For substrate preparation and printing, Toluene, chloroform, ethanol, dimethyl sulfoxide (DMSO), and glycerol were purchased from Sigma-Aldrich (Germany). Tamra amine, 5-isomer (NH2-Tamra) was bought from Lumiprobe GmbH (Germany). Thiol-PEG-Biotin, MW 2000 (SH-Biotin) and Fluorescein-PEG-Thiol (SH-FITC) were obtained from Nanocs Company (USA). Biotin-dPEG7-NH2 (NH2-biotin), streptavidin Cyanine-3 (SA-Cy3), 3-methacryloxypropyltrimethoxysilane (MPS-silane), bovine serum albumin (BSA), and phosphate-buffered saline (PBS) were obtained from Sigma-Aldrich (Germany). A 10X PBS solution contains NaCl: 1.37 M, KCl: 27 mM, Na2HPO4: 100 mM, KH2PO4: 18 mM.
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