F4 80 pe vio 770

Manufactured by Miltenyi Biotec

F4/80‐PE‐Vio 770 is a fluorescently-labeled antibody for the detection of the F4/80 antigen, which is expressed on mouse macrophages. It can be used in flow cytometry applications.

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F4/80 (13 citations) F4/80-PE (2 citations)

2 protocols using f4 80 pe vio 770

Flow Cytometry Characterization of Immune Cells

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MHC Class II‐VioBlue (Miltenyi Biotec, Cat.# 130‐112‐237, clone: REA813, 1:50), CD206 (mannose receptor)‐Brilliant Violet 711 (BioLegend, Cat.# 141727, clone: C068C2, 1:40), CD11b‐Vio Bright FITC (Miltenyi Biotec, Cat.# 130‐113‐805, clone: REA592, 1:50), CD45‐PerCP‐Vio 700 (Miltenyi Biotec, Cat.# 130‐110‐663/130‐110‐801, clone: REA737, 1:50), F4/80‐PE‐Vio 770 (Miltenyi Biotec, Cat.# 130‐118‐459, clone: REA126, 1:50), Gr1‐APC (Miltenyi Biotec, Cat.# 130‐112‐307, clone: REA810, 1:50), and LIVE/DEAD Fixable Yellow Dead Cell Stain (Thermo Fisher Scientific, Cat.# L34967, 1:1000).

Matos A.I., Peres C., Carreira B., Moura L.I., Acúrcio R.C., Vogel T., Wegener E., Ribeiro F., Afonso M.B., Santos F.M., Martínez‐Barriocanal Á., Arango D., Viana A.S., Góis P.M., Silva L.C., Rodrigues C.M., Graca L., Jordan R., Satchi‐Fainaro R, & Florindo H.F. (2023). Polyoxazoline‐Based Nanovaccine Synergizes with Tumor‐Associated Macrophage Targeting and Anti‐PD‐1 Immunotherapy against Solid Tumors. Advanced Science, 10(25), 2300299.

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Multicolor Flow Cytometry Analysis of Immune Cell Subsets

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Two different stains were performed with fluorochrome-conjugated antibodies. The first staining consisted of CD45R (B220) PE (Miltenyi Biotech, REA755); CD4 FITC (Miltenyi Biotech, GK1.5); CD8 PerCP-Vio 700 (Miltenyi Biotech, 53–6.7); CD69 APC-Vio 770 (Miltenyi Biotech, H1.2F3); CD44 APC (Miltenyi Biotech, IM7.8.1). The second staining included CD11b APC (Miltenyi Biotech, M1/70); CD103 PE (Miltenyi Biotech, REA789); F4/80 PE-Vio 770 (Miltenyi Biotech, REA126); MHCII APC-Vio 770 (Miltenyi Biotech, REA813). Fc receptors were blocked with Anti-mCD16/CD32 (BD). Only events that appeared single in forward-scatter width were analyzed. FACSCanto II and FACSDiva software (BD) were used for flow cytometry and data were analyzed using FlowJo software (TreeStar).

Peña-Cearra A., Pascual-Itoiz M.A., Lavín J.L., Fuertes M., Martín-Ruiz I., Castelo J., Palacios A., Barriales D., Fullaondo A., Aransay A.M., Rodríguez H., Anguita J, & Abecia L. (2022). Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis. Scientific Reports, 12, 9977.

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