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Acc antibody 3662

Manufactured by Cell Signaling Technology
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The ACC antibody (#3662) from Cell Signaling Technology is a primary antibody that recognizes acetyl-CoA carboxylase (ACC), an enzyme involved in fatty acid synthesis and metabolism. This antibody can be used for applications such as western blotting and immunohistochemistry.

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Lab products found in correlation

α tubulin sc 23948, by Santa Cruz Biotechnology (2 mentions) Mk 2206, by Active Motif (2 mentions) Polyclonal sin1 antibody a300 910a, by Fortis Life Sciences (2 mentions) Oil red o, by Merck Group (2 mentions) Chir99021, by LC Laboratories (2 mentions) β actin, by Merck Group (1 mentions)

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ACC antibody (2 citations)

3 protocols using acc antibody 3662

1

Investigating mTOR and GSK3 Signaling

Cited 1 time
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All mTOR kinase inhibitors, the GSK3 inhibitor SB216763, the proteasome inhibitor MG132, the protein synthesis inhibitor cycloheximide (CHX) and the antibodies against GSK3α/β, phospho-GSK3α/β (Ser21/9), phospho-Akt (Ser473), Akt, rictor and raptor were the same as described previously 22 (link). The GSK3 inhibitor CHIR99021 was purchased from LC laboratories (Woburn, MA). BKM120 was supplied by Novartis Pharmaceuticals Corporation (East Hanover, NJ). API-1 (NSC177233) was obtained from the National Cancer Institute (Bethesda, MD). MK2206 was purchased from Active Biochem (Maplewood, NJ). Perifosine was supplied by Keryx Biopharmaceuticals, Inc (New York, NY). Oil Red O was purchased from Sigma Chemical Co. (St. Louis, MO). SREBP1 (sc-13551), FASN (sc-55580) and α-tubulin (sc-23948) antibodies were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). ACC antibody (#3662) was purchased from Cell Signaling Technology, Inc. (Beverly, MA). Polyclonal Sin1 antibody (A300-910A) was purchased from Bethyl Laboratories, Inc. (Montgomery, TX).
Li S., Oh Y.T., Yue P., Khuri F.R, & Sun S.Y. (2015). Inhibition of mTOR complex 2 induces GSK3/FBXW7-dependent degradation of sterol regulatory element-binding protein 1 (SREBP1) and suppresses lipogenesis in cancer cells. Oncogene, 35(5), 642-650.
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2

Investigating mTOR and GSK3 Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All mTOR kinase inhibitors, the GSK3 inhibitor SB216763, the proteasome inhibitor MG132, the protein synthesis inhibitor cycloheximide (CHX) and the antibodies against GSK3α/β, phospho-GSK3α/β (Ser21/9), phospho-Akt (Ser473), Akt, rictor and raptor were the same as described previously 22 (link). The GSK3 inhibitor CHIR99021 was purchased from LC laboratories (Woburn, MA). BKM120 was supplied by Novartis Pharmaceuticals Corporation (East Hanover, NJ). API-1 (NSC177233) was obtained from the National Cancer Institute (Bethesda, MD). MK2206 was purchased from Active Biochem (Maplewood, NJ). Perifosine was supplied by Keryx Biopharmaceuticals, Inc (New York, NY). Oil Red O was purchased from Sigma Chemical Co. (St. Louis, MO). SREBP1 (sc-13551), FASN (sc-55580) and α-tubulin (sc-23948) antibodies were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). ACC antibody (#3662) was purchased from Cell Signaling Technology, Inc. (Beverly, MA). Polyclonal Sin1 antibody (A300-910A) was purchased from Bethyl Laboratories, Inc. (Montgomery, TX).
Li S., Oh Y.T., Yue P., Khuri F.R, & Sun S.Y. (2015). Inhibition of mTOR complex 2 induces GSK3/FBXW7-dependent degradation of sterol regulatory element-binding protein 1 (SREBP1) and suppresses lipogenesis in cancer cells. Oncogene, 35(5), 642-650.
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3

Tissue-Specific Protein Expression Analysis

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Whole-cell lysates of liver, IWAT, and BAT were prepared by homogenizing tissue in buffer (150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 10 mM Tris, 1% Triton X-100, 0.5% NP-40), and protein was quantitated by Lowry assay. Fifty microgram of whole-cell extracts was used for liver SCD1, 35 μg for liver FAS, 30 μg for liver ACC and IWAT UCP1, and 3 μg for BAT UCP1. Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes. Expression of liver SCD1, FAS, ACC, and IWAT UCP1 were detected and standardized to β-actin (Sigma Millipore, St. Louis, MO; A-5441). The FASN (sc-48357) and SCD-1 (sc-14720; E-15) antibodies were from Santa Cruz Biotechnology (Dallas, TX), whereas the ACC antibody (#3662) was from Cell Signaling (Danvers, MA). PDC-E2 (sc-271534) was used as loading control for BAT UCP1 as described previously (Wanders et al., 2015 (link)).
Fang H., Stone K.P., Forney L.A., Sims L.C., Gutierrez G.C., Ghosh S, & Gettys T.W. (2021). Implementation of dietary methionine restriction using casein after selective, oxidative deletion of methionine. iScience, 24(5), 102470.
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