Prime 95b emccd camera

Manufactured by Yokogawa

The Prime 95B EMCCD camera is a high-performance imaging device designed for low-light applications. It features a large sensor size, high quantum efficiency, and on-chip electron-multiplying gain to provide exceptional sensitivity and signal-to-noise ratio. The camera is capable of capturing images and video at a range of frame rates and resolutions, making it suitable for a variety of scientific and industrial applications.

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Lab products found in correlation

Csu x1 confocal scanner unit, by Yokogawa (2 mentions) Csu x1, by Yokogawa (2 mentions) Axiocam mrm camera, by Zeiss (1 mentions) Axioplan 2 microscope, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) 1.2 na water objective, by Nikon (1 mentions) Lambda 10 b smart shutter, by Sutter Instruments (1 mentions) Intensilight c hgfi 130 w mercury lamp, by Sutter Instruments (1 mentions)

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Prime 95B (3 citations)

4 protocols using prime 95b emccd camera

Imaging Dissected Gonads in C. elegans

Cited 1 time

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Antibody staining of dissected gonads was as previously described (Starich et al., 2014 (link)). DIC and fluorescent images were acquired on a Zeiss (Thornwood, NY) motorized Axioplan 2 microscope with either a 40x Plan-Neofluar (numerical aperture 1.3), a 63x Plan-Apochromatic (numerical aperture 1.4), or 100x PlanApochromatic (N.A. 1.4) objective lens using an AxioCam MRm camera and AxioVision software (Zeiss).
For fasn-1(av138[fasn-1::gfp]) imaging data, animals were immobilized on 7% agarose pads with 0.05 μm polystyrene beads and imaged using a spinning disk confocal system with a Nikon 60 × 1.2 NA water objective, a Photometrics Prime 95B EMCCD camera, and a Yokogawa CSU-X1 confocal scanner unit. Images were acquired and analyzed by Nikon’s NIS imaging software and ImageJ/FIJI Bio-formats plugin (National Institutes of Health) (Linkert et al., 2010 (link); Schindelin et al., 2012 (link)). The acquisition of GFP and DIC images was performed with 1 μm z-step size and 15–20 z planes.

Starich T.A., Bai X, & Greenstein D. (2020). Gap junctions deliver malonyl-CoA from soma to germline to support embryogenesis in Caenorhabditis elegans. eLife, 9, e58619.

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Spinning Disk Confocal Imaging Protocol

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All imaging was carried out on a spinning disk confocal system that uses a Nikon 60×1.2 NA water or oil objectives, a Photometrics Prime 95B EMCCD camera and a Yokogawa CSU-X1 confocal scanner unit. Nikon's NIS imaging software were applied to capture the images. The image data were processed using ImageJ/FIJI Bio-formats plug-in (National Institutes of Health) (Schindelin et al., 2012 (link); Linkert et al., 2010 (link)).

Bai X., Smith H.E., Romero L.O., Bell B., Vásquez V, & Golden A. (2024). A mutation in F-actin polymerization factor suppresses the distal arthrogryposis type 5 PIEZO2 pathogenic variant in Caenorhabditis elegans. Development (Cambridge, England), 151(4), dev202214.

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Live Imaging of Anesthetized Samples

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All live imaging and DAPI staining slides were imaged by a spinning disk confocal microscope system, including an inverted Nikon Eclipse microscope, a Photometrics Prime 95B EMCCD camera, and a Yokogawa CSU-X1 confocal scanner unit. Images were acquired by Nikon's NIS imaging software using a Nikon 60 × 1.2 NA water objective. For DIC imaging, the tested animals were immobilized on 7% agar pads with an anesthetic (0.01% levamisole in M9 buffer). DIC image acquisition was performed using a Nikon 60 × 1.2 NA water objective with 1-μm z-step size; 15–20 planes were captured.

Bai X, & Golden A. (2023). Transmembrane protein 120A (TMEM-120A/TACAN) coordinates with PIEZO channel during Caenorhabditis elegans reproductive regulation. G3: Genes|Genomes|Genetics, 14(1), jkad251.

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Live Imaging of Calcium Dynamics

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Live imaging was performed on a spinning disk confocal system that uses a Nikon 60 × 1.2 NA water objective, a Photometrics Prime 95B EMCCD camera, and a Yokogawa CSU-X1 confocal scanner unit. Images were acquired and analyzed by Nikon’s NIS imaging software and ImageJ/FIJI Bio-formats plugin (National Institutes of Health) (Linkert et al., 2010 (link); Schindelin et al., 2012 (link)). GCaMP3 images were also acquired by a 60×/1.40 NA oil-immersion objective on a Nikon Eclipse 80i microscope equipped with a SPOT RT39M5 sCMOS camera (Diagnostic Instruments, Sterling Heights, MI, USA) with a 0.63x wide field adapter, controlled by SPOT Advanced imaging software (v. 5.0) with Peripheral Devices and Quantitative Imaging modules. Images were acquired at 2448 × 2048 pixels, using the full camera chip, and saved as 8-bit TIFF files. Fluorescence excitation was provided by a Nikon Intensilight C-HGFI 130 W mercury lamp and shuttered with a Lambda 10-B SmartShutter (Sutter Instruments, Novato, CA), also controlled through the SPOT software. Single-channel GCaMP time-lapse movies were acquired using a GFP filter set (470/40 × 495 lpxr 525/50 m) (Chroma Technologies, Bellows Falls, VT) at 1 frame per second, with an exposure time of 40–60 ms, gain of 8, and neutral density of 16.

Bai X., Bouffard J., Lord A., Brugman K., Sternberg P.W., Cram E.J, & Golden A. (2020). Caenorhabditis elegans PIEZO channel coordinates multiple reproductive tissues to govern ovulation. eLife, 9, e53603.

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