Cells were stained with anti-CD31-PE, anti-CD33-FITC, anti-CD34-PE, anti-CD45-FTIC, anti-CD13-PE, anti-CD44-PE, anti-CD45-FITC, anti-CD71-PE, anti-CD90-PE, anti-CD95-APC, anti-HLA-ABC-FITC, anti-HLA-DR-FITC (all from BD Bioscience), anti-CD31-APC (eBioscience), anti-CD13-PE (Biolegend) and anti-CD105-FITC (R&D Systems). The stained cells were analyzed with a FACSCalibur flow cytometer (Becton Dickinson).
Anti cd105 fitc
Manufactured by R&D Systems
Sourced in United States
Anti-CD105-FITC is a fluorescently labeled antibody that recognizes the CD105 (endoglin) cell surface antigen. CD105 is a transmembrane glycoprotein that is expressed on endothelial cells and involved in angiogenesis. The FITC label allows for the detection of CD105-positive cells using flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd45 fitc, by BD (2 mentions)
Anti cd90 pe, by BD (2 mentions)
Anti cd34 pe, by BD (2 mentions)
Anti hla abc fitc, by BD (2 mentions)
Anti hla dr fitc, by BD (2 mentions)
Anti cd13 pe, by BioLegend (2 mentions)
Facscalibur flow cytometer, by BD (2 mentions)
Anti cd31 apc, by Thermo Fisher Scientific (1 mentions)
Anti cd44 pe, by BD (1 mentions)
Anti cd31 pe, by BD (1 mentions)
Anti cd13 pe, by BD (1 mentions)
Anti cd71 pe, by BD (1 mentions)
Anti cd95 apc, by BD (1 mentions)
Anti hlag, by Abcam (1 mentions)
Anti cd90 fitc, by BD (1 mentions)
Anti cd73 fitc, by BioLegend (1 mentions)
Facscanto 2, by BD (1 mentions)
Anti cd90 pe, by R&D Systems (1 mentions)
Nh media, by Miltenyi Biotec (1 mentions)
Anti cd73 pe, by BD (1 mentions)
4 protocols using anti cd105 fitc
1
Multiparametric Flow Cytometry Immunophenotyping
2
Phenotyping Cell-Surface Antigens in BM-MSCs
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To phenotype cell-surface antigens, BM-MSCsPRL−1 (passage no. 3) were incubated with each of the following monoclonal antibodies: anti-CD34-PE, anti-CD90-PE, anti-HLA-ABC-FITC, anti-HLA-DR-FITC (BD Bioscience, San Jose, CA, USA), anti-CD13-PE (BioLegend, San Diego, CA, USA), anti-CD105-FITC (R&D Systems, Abingdon, UK), and anti-HLAG (Abcam). The phenotype of each cell line was analyzed using a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA, USA).
3
Immunophenotyping of Mesenchymal Stem Cells
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The expression of MSC surface markers were assessed by flow cytometry analysis. Cells were trypsinized into a single cell suspension and incubated with the fluorochrome-conjugated antibodies against human antigens, including anti-CD34-PE, anti-CD45-FITC, anti-CD90-FITC (BD Biosciences), anti-CD73-FITC (Bio Legend) and anti-CD105-FITC (R&D systems) for 15 mins in the dark at RT. After incubation, cells were washed with 1X PBS, centrifuged at 1500 rpm for 5 minutes and suspended in 1X PBS for flow cytometry analysis. Samples were acquired on a FACS canto II flow cytometer (BD Biosciences) and data were analyzed using FlowJo software (Tree Star).
4
Phenotyping and Multilineage Differentiation of ASCs
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ASCs were defined as proposed by the ISCT committee. Briefly, their phenotype was determined by flow cytometry (MACSQuant, Miltenyi Biotec, Germany) using the following monoclonal antibodies: anti-CD45-FITC and anti-HLA-DR-PE (Exalpha Biologicals, Maynard, MA), anti-CD73-PE were purchased from (BD Biosciences, San Diego, CA), anti CD14-PE, anti-CD19-PE, anti-CD105-FITC, and anti-CD90-PE were purchased from (R&D systems, Minneapolis, MN). The trilineage capacity of ASCs was confirmed by inducing their differentiation into adipogenic, osteogenic and chondrogenic lineages using appropriate culture conditions (NH media; Miltenyi Biotec).
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