Ferret interferon gamma elispot kit

Manufactured by Mabtech

Sourced in Sweden

The Ferret interferon-gamma ELISpot kit is a laboratory tool designed to detect and quantify the production of interferon-gamma by ferret cells. The kit utilizes the enzyme-linked immunospot (ELISpot) assay technique to facilitate the analysis of cellular immune responses.

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Lab products found in correlation

Pre coated plates, by Mabtech (2 mentions)

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ELISpot kit (56 citations)

2 protocols using ferret interferon gamma elispot kit

Influenza Virus-Specific T Cell Responses

Cited 1 time

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Lung MNC were assessed for responses to A/California/07/2009 (H1N1) and A/Perth/16/2009 (H3N2)^{18 (link)}. Both viruses were used at a multiplicity of infections of 0.08. Following overnight stimulation, IFN-γ expressing cells were detected using the Ferret interferon-gamma ELISpot kit with pre-coated plates (Mabtech, Nacka, Sweden). Results from duplicate tests were averaged. Data were analysed by subtracting the mean number of spots in the control wells (cells and allantoic fluid) from the mean counts of spots in wells with cells and antigen.

Marriott A.C., Gooch K.E., Brown P.J., Ryan K.A., Jones N.J., Merredew N., Wiblin N., Dibben O., Bright H., Hallis B., Whittaker C.J, & Carroll M.W. (2021). Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine. NPJ Vaccines, 6, 43.

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PBMC and Lung MNC Interferon-gamma ELISpot

Cited 1 time

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PBMC and lung MNC were assayed as described previously^{19 (link)}. In brief, PBMC and lung MNC were assessed for responses to A/California/07/2009 (H1N1) and A/Perth/16/2009 (H3N2). Both viruses were used at an MOI of 0.08. Following overnight stimulation, IFN-γ expressing cells were detected using the Mabtech Ferret interferon-gamma ELISpot kit with pre-coated plates (Mabtech, Nacka, Sweden). Results from duplicate tests were averaged. Data were analysed by subtracting the mean number of spots in the control wells (cells and allantoic fluid) from the mean counts of spots in wells with cells and antigen.

Gooch K.E., Marriott A.C., Ryan K.A., Yeates P., Slack G.S., Brown P.J., Fothergill R., Whittaker C.J, & Carroll M.W. (2019). Heterosubtypic cross-protection correlates with cross-reactive interferon-gamma-secreting lymphocytes in the ferret model of influenza. Scientific Reports, 9, 2617.

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