As previously described, peripheral blood was collected in S-Monovette K3 EDTA blood collection tubes (Sarstedt) (Anft et al., 2020 (link); Paniskaki et al., 2022 (link)). Collected blood was prediluted in PBS/BSA (Gibco) at a 1:1 ratio and underlaid with 15 mL of Ficoll-Paque Plus (GE Healthcare). Tubes were centrifuged at 800 g for 20 min at room temperature. Isolated PBMCs were washed twice with PBS/BSA and stored at −80°C until use. The cryopreserved PBMCs were thawed by incubating cryovials 2–3 min at 37°C in bead bath, washed twice in 37°C RPMI 1640 media (Life Technologies) supplemented with 1% penicillin–streptomycin-glutamine (Sigma-Aldrich), and 10% fetal calf serum (PAN-Biotech) medium, and incubated overnight at 37°C.
S monovette k3 edta blood collection tubes
Manufactured by Sarstedt
Sourced in Germany
The S-Monovette K3 EDTA blood collection tubes are used for the collection and transportation of venous blood samples. These tubes contain the anticoagulant K3 EDTA, which prevents blood from clotting during the collection process.
Automatically generated - may contain errors
Lab products found in correlation
Pbs bsa, by Thermo Fisher Scientific (5 mentions)
Ficoll paque plus, by GE Healthcare (5 mentions)
Penicillin streptomycin glutamine (psg), by Merck Group (4 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by PAN Biotech (1 mentions)
5 protocols using s monovette k3 edta blood collection tubes
1
Isolation and Cryopreservation of PBMCs
2
Isolation and Cryopreservation of PBMCs
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As previously described (20 (link)), peripheral blood was collected in S-Monovette K3 EDTA blood collection tubes (Sarstedt). Collected blood was prediluted in PBS/BSA (Gibco) at a 1:1 ratio and underlaid with 15 mL of Ficoll-Paque Plus (GE Healthcare). Tubes were centrifuged at 800g for 20 min at room temperature. Isolated PBMCs were washed twice with PBS/BSA and stored at -80°C until use. The cryopreserved PBMCs were thawed by incubating cryovials 2-3 minutes at 37°C in bead bath, washed twice in 37°C RPMI 1640 media (Life Technologies) supplemented with 1% penicillin-streptomycin-glutamine (Sigma-Aldrich), and 10% fetal calf serum (PAN-Biotech) medium, and incubated overnight at 37°C.
3
Isolation and Cryopreservation of PBMCs
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As previously described, peripheral blood was collected in S-Monovette K3 EDTA blood collection tubes (Sarstedt) (22 (link)). Collected blood was prediluted in PBS/BSA (Gibco) at a 1:1 ratio and underlaid with 15 mL of Ficoll-Paque Plus (GE Healthcare). Tubes were centrifuged at 800g for 20 min at room temperature. Isolated PBMCs were washed twice with PBS/BSA and stored at -80°C until use. The cryopreserved PBMCs were thawed by incubating cryovials 2-3 minutes at 37°C in bead bath, washed twice in 37°C RPMI 1640 media (Life Technologies) supplemented with 1% penicillin-streptomycin-glutamine (Sigma-Aldrich), and 10% fetal calf serum (FCS) (PAN-Biotech) medium, and incubated overnight at 37°C.
4
Isolation of Peripheral Blood Mononuclear Cells
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Peripheral blood was collected in S-Monovette K3 EDTA blood collection tubes (Sarstedt). Collected blood was pre-diluted in PBS/BSA (Gibco) at a 1:1 ratio and underlaid with 15 mL of Ficoll-Paque Plus (GE Healthcare). Tubes were centrifuged at 800 × g for 20 min at room temperature. Isolated peripheral blood mononuclear cells (PBMCs) were washed twice with PBS/BSA and stored at −80°C until use as previously described.59 (link)
5
Isolation and Cryopreservation of PBMCs
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Peripheral blood was collected in S-Monovette K3 EDTA blood collection tubes (Sarstedt, Germany). Collected blood was prediluted in phosphate-buffered saline (PBS/BSA) (Gibco, USA) at a 1:1 ratio and underlaid with 15 mL of Ficoll-Paque Plus (GE Healthcare, Germany). Tubes were centrifuged at 800 g for 20 minutes at room temperature. Isolated PBMCs were washed twice with PBS/BSA and stored at -80°C. The cryopreserved PBMCs were thawed by incubating cryovials for 2 to 3 minutes at 37°C in a bead bath, washed twice in 37°C RPMI 1640 medium (Life Technologies, USA) supplemented with 1% penicillin-streptomycin-glutamine (Sigma–Aldrich, USA) and 10% fetal calf serum (PAN-Biotech, Germany), and incubated overnight at 37°C.
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