The cultured cells were labeled with the TUNEL Bright Red Apoptosis Detection Kit System (A113-01/02/03, Vazyme Biotech, China). Specimens were viewed on a CX31-32RFL confocal microscope. The cells undergoing apoptosis and the total number of cells were measured (Image J software). The apoptotic ratio was apoptotic cells/total cells 100% [28 (link)].
Tunel bright red apoptosis detection kit system
Manufactured by Vazyme
Sourced in United States
The TUNEL Bright Red Apoptosis Detection Kit System is a laboratory tool designed to detect and visualize apoptosis, a form of programmed cell death, in biological samples. The kit employs the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) method to label DNA strand breaks, which is a hallmark of apoptosis. The bright red fluorescent dye used in the kit enables the visualization of apoptotic cells under a fluorescence microscope.
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3 protocols using tunel bright red apoptosis detection kit system
1
TUNEL Apoptosis Detection in Cells
2
Apoptosis Detection via TUNEL Assay
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Sections of brain and cultured cells were labeled with the TUNEL Bright Red Apoptosis Detection Kit System (Vazyme Biotech, US). Specimens were viewed on a CX31-32RFL confocal microscope. Cells undergoing apoptosis and the total number of cells were measured (Image J software). The apoptotic ratio was : apoptotic cells / total cells × 100%.
3
Apoptosis Detection in Brain Cells
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Sections of the brain and cultured cells were labeled with the TUNEL Bright Red Apoptosis Detection Kit System (Vazyme Biotech, USA) in accordance with the instructions of the TUNEL kit. The sections were observed by Laser Scanning Confocal Microscope (CX31-32RFL, Olympus, Tokyo, Japan). The apoptotic cells were counted by Image J software (ver. 1.61). The apoptotic ratio was calculated as apoptotic cells / total cells × 100%.
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