Log In Sign up
The largest database of trusted experimental protocols

Mab452

Manufactured by R&D Systems
Visit Supplier
Sourced in United States

MAB452 is a Mouse Monoclonal Antibody produced by R&D Systems. It is intended for use in Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blot applications.

Automatically generated - may contain errors

Lab products found in correlation

Mab453, by R&D Systems (1 mentions) Cycloheximide (chx), by Abcam (1 mentions) Hy 13982, by MedChemExpress (1 mentions) Sb225002, by MedChemExpress (1 mentions) Mg132, by Selleck Chemicals (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Sds page gel, by Bio-Rad (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Phosphatase inhibitor cocktail, by Cell Signaling Technology (1 mentions) Chemodoc xrs, by Bio-Rad (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Cytoselect 96 well cell migration assay kit, by Cell Biolabs (1 mentions) Be0185, by BioXCell (1 mentions)

4 protocols using mab452

1

Bone Marrow Labeling by Microinjection

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow tagging by microinjection were performed as previously described29 (link). Microinjection of the tibia marrow was performed sequentially under 0.3% pentobarbital anesthesia, using a 1 ml syringe mounted with a 5 mm-outer-diameter veterinary needle. For each procedure aseptic technique was used. For the tibia marrow injection, we injected at the chosen injection site of tibia plateau just below the knee and the bone wall was perforated with a veterinary needle. The red cell tracker or CXCL1/2 antibodies (Clone # 48415, R&D Systems, MAB453, 0.2 μg/ml or 5 ng/mice once; Clone # 40605, R&D Systems, MAB452, 2 μg/ml or 40 ng/mice once) was injected in the marrow cavity over 30 s. Animals were sacrificed 12 h after injection.
Yu Y., Wang R.R., Miao N.J., Tang J.J., Zhang Y.W., Lu X.R., Yan P.Y., Wang J, & Jia X.M. (2022). PD-L1 negatively regulates antifungal immunity by inhibiting neutrophil release from bone marrow. Nature Communications, 13, 6857.
+ Open protocol
+ Expand
2

Investigating Immunotherapy Responses in Prostate Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
The chemicals used in vitro and in vivo assays are as follows: SB225002 (0.1 μM for the in vitro assays and 2 mg/kg for the mouse treatment, MedChemExpress; HY-16711), JSH-23 (5 μM for the in vitro assays and 2 mg/kg for the mouse treatment, MedChemExpress; HY-13982); RS504393 (0.2 μM for the in vitro assays, MedChemExpress; HY-15418); MG132 (Selleck Chemical; S2619s); cycloheximide (BioVision; 1041-1 G). SB225002 and JSH-23 in DMSO were diluted in corn oil for in vivo administration through intraperitoneal injection every other day or gavage daily, respectively. For immunotherapy, antibody intraperitoneal injection was started when subcutaneous tumor volume reached ~100 mm3 or PtenPC−/−; Arid1aPC−/− mice were 2.5-month-old. The following antibodies were injected alone or in combination: anti-mouse PD1 (BioXCell; Clone: RMP1-14, BE0146); anti-mouse CTLA4 (BioXCell; Clone: 9H10, BE0131); anti-mouse Ly6G (BioXCell; clone: 1A8, BE0075-1); and their respective isotype IgG controls. PD1/CTLA4 antibody were simultaneously administrated to the mice. Treatment was administered twice a week through intraperitoneal injections at a dosage of 200 μg/injection/antibody, and subcutaneous tumor volume was monitored every 4 days. CXCL2 was depleted from conditioned media by incubation with mouse antibody against Mip2/Cxcl2 (R&D Systems; MAB452).
Li N., Liu Q., Han Y., Pei S., Cheng B., Xu J., Miao X., Pan Q., Wang H., Guo J., Wang X., Zhang G., Lian Y., Zhang W., Zang Y., Tan M., Li Q., Wang X., Xiao Y., Hu G., Jiang J., Huang H, & Qin J. (2022). ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression. Nature Communications, 13, 7281.
+ Open protocol
+ Expand
3

Western Blot Analysis of CXCL2 and β-actin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were collected and lysed by RIPA Buffer (Thermo Scientific, USA) supplemented with Complete Protease Inhibitor Cocktail (Roche, Switzerland) and Phosphatase Inhibitor Cocktail (Cell Signaling Technology, USA). The lysates were separated on SDS-PAGE gel (BioRad Laboratories) and transferred to PVDF membranes (Millipore, USA). Membranes were blocked in 5% BSA for 1 h at room temperature and incubated with primary antibodies at 4 °C overnight. Secondary antibodies were incubated for 2 h at room temperature, and the immunoblots were visualized on a BioRad ChemoDoc XRS + imaging system. Antibodies against CXCL2 (MAB452) was purchased from R&D Systems (USA) and β-actin (#4970) from Cell Signaling Technology. Primary antibodies are listed in Supporting Information Table S1.
Chan Y.T., Tan H.Y., Lu Y., Zhang C., Cheng C.S., Wu J., Wang N, & Feng Y. (2023). Pancreatic melatonin enhances anti-tumor immunity in pancreatic adenocarcinoma through regulating tumor-associated neutrophils infiltration and NETosis. Acta Pharmaceutica Sinica. B, 13(4), 1554-1567.
+ Open protocol
+ Expand
4

Monocyte Chemotaxis Towards Aged Fat Transplants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monocytes were isolated from the bone marrow of young mice by immunomagnetic negative selection (StemCell, Cat#19861). Boyden chambers, specifically Cell Biolabs CytoSelect™ 96‐well Cell Migration Assay Kit (5 μm pore size, Cat# CBA‐105‐5) was used to investigate the chemotaxis of monocytes toward the culture media of young or aged fat transplants. The assay was performed according to the manufacturer's instructions. Briefly, 100 μl of 0.5–1.0 × 105 cells/ml bone marrow derived monocytes were loaded in the membrane chamber in serum free media, and 150 μl of tissue culture media from young or aged fat transplants was added to the wells of the feeder tray as chemoattractant. By the end of the incubation, the migrated cells were lysed in 4× Lysis Buffer/CyQuant® GR dye solution, and fluorescence was read with a fluorescence plate reader at 480 nm/520 nm. Anti‐CCL2 (100 μg/ml, Bio X Cell, Cat# BE0185), anti‐TNFα (25 μg/ml, Bio X Cell, Cat# BE0058), and/or anti‐CXCL2 (50 μg/ml, R&D Systems, Cat# MAB452) or isotype control were used to block the corresponding cytokine(s) in the culture media. Serum‐free medium was used as negative control for the culture media.
Song J., Farris D., Ariza P., Moorjani S., Varghese M., Blin M., Chen J., Tyrrell D., Zhang M., Singer K., Salmon M, & Goldstein D.R. (2023). Age‐associated adipose tissue inflammation promotes monocyte chemotaxis and enhances atherosclerosis. Aging Cell, 22(2), e13783.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!