Pe labeled anti mouse cd8a clone 53 6.7 rat igg2aκ

Manufactured by BioLegend

Sourced in United States

PE-labeled anti-mouse CD8a (clone 53–6.7) rat IgG2aκ is a primary antibody that binds to the CD8a protein expressed on the surface of mouse cytotoxic T cells. The antibody is conjugated with the fluorescent dye Phycoerythrin (PE) which can be detected using flow cytometry.

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Lab products found in correlation

Streptavidin fitc, by BD (2 mentions) Pe cy5 labeled anti mouse cd11c clone n418 hamster igg, by BioLegend (2 mentions) Fitc labeled anti mouse cd3ε clone 145 2c11 hamster igg, by BioLegend (2 mentions) Fitc labeled anti mouse cd25 clone pc61 rat igg1λ, by BioLegend (2 mentions) Pe cy5 labeled anti mouse cd4 clone gk1.5 rat igg2bκ, by BioLegend (2 mentions) Pe labeled anti mouse nk1.1 clone pk136 mouse igg2aκ, by BD (2 mentions) Biotin fasl clone mfl3 armenian hamster igg2, by BD (2 mentions) Annexin 5 fitc, by BD (2 mentions)

Close spelling variants of this product

Anti-CD8a (clone 53–6.7; Anti-mouse CD8a (clone 53-6.7, CD8a (clone 53-6.7, CD8a-PE (53-6.7, PE mouse anti-rat CD8a Anti-CD8a (53-6.7, PE-labeled anti-CD8a CD8a (53-6.7, PE-anti-rat CD8a PE anti-mouse CD8a

2 protocols using pe labeled anti mouse cd8a clone 53 6.7 rat igg2aκ

Flow Cytometry Analysis of Immune Cells

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Antibodies used in the flow cytometry analysis were as follows: fluorescein isothiocyanate (FITC)-labeled anti-mouse Ly-6G/Ly-6C (Gr-1; clone RB6-8C5) rat IgG2bκ, phycoerythrin (PE)-labeled anti-mouse F4/80 (clone CIA3-1) rat IgG2bκ, PE/Cy5-labeled anti-mouse CD11c (clone N418) hamster IgG, FITC-labeled anti-mouse CD3ε (clone 145–2C11) hamster IgG, PE/Cy5-labeled anti-mouse CD45R/B220 (clone RA3-6B2) rat IgG2aκ, FITC-labeled anti-mouse CD25 (clone PC61) rat IgG1λ, PE-labeled anti-mouse CD8a (clone 53–6.7) rat IgG2aκ, and PE/Cy5-labeled anti-mouse CD4 (clone GK1.5) rat IgG2bκ were from BioLegend, Inc. (San Diego, CA, USA). PE-labeled anti-mouse NK1.1 (clone PK136) mouse IgG2aκ, biotin-FasL (clone MFL3) Armenian hamster IgG2, FITC-streptavidin and FITC-annexin V were from BD Pharmingen (San Diego, CA, USA). Monocytes/macrophages were defined as SSC^hiF4/80^hiGr-1lo population, granulocytes as SSC^hiGr-1^hi cells and dendritic cells as SSC^loF4/80-cells. B cells, T cells, NK cells, NKT cells were defined as SSC^lo CD45R+, SSC^loCD3ε+NK1.1-, SSC^loCD3ε+NK1.1+, and SSC^loCD3ε+NK1.1+, respectively. CD4 T cells (CD4+CD8-), CD8 T cells (CD4-CD8+), and regulatory T cells (CD4+CD8-CD25+) were also counted. Values represent the percentage of the non-parenchymal cells in each organ.

Uchida A., Yasuma T., Takeshita A., Toda M., Okano Y., Nishihama K., D’Alessandro-Gabazza C.N., Fridman D’Alessandro V., Inoue C., Takagi T., Mukaiyama H., Takagi N., Shimizu K., Yano Y, & Gabazza E.C. (2021). Oral Limonite Supplement Ameliorates Glucose Intolerance in Diabetic and Obese Mice. Journal of Inflammation Research, 14, 3089-3105.

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Multicolor Flow Cytometry Analysis of Immune Cells

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The percentage of spleen cells was evaluated by flow cytometry (BD Biosciences, Oxford, UK) using the following antibodies: fluorescein isothiocyanate (FITC)-labeled anti-mouse Ly-6G/Ly-6C (Gr-1; clone RB6-8C5) rat IgG2bκ, phycoerythrin (PE)-labeled anti-mouse F4/80 (clone CIA3-1) rat IgG2bκ, PE/Cy5-labeled anti-mouse CD11c (clone N418) hamster IgG, FITC-labeled anti-mouse CD3ε (clone 145-2C11) hamster IgG, PE/Cy5-labeled anti-mouse CD45R/B220 (clone RA3-6B2) rat IgG2aκ, FITC-labeled anti-mouse CD25 (clone PC61) rat IgG1λ, PE-labeled anti-mouse CD8a (clone 53-6.7) rat IgG2aκ, and PE/Cy5-labeled anti-mouse CD4 (clone GK1.5) rat IgG2bκ, which were from BioLegend, Inc. (San Diego, CA). PE-labeled anti-mouse NK1.1 (clone PK136) mouse IgG2aκ, biotin-FasL (clone MFL3) Armenian hamster IgG2, FITC-streptavidin, and FITC-annexin V were from BD Pharmingen. Monocytes/macrophages were defined as SSC^hiF4/80^hiGr-1lo population, granulocytes as SSC^hiGr-1^hi cells, and dendritic cells as SSC^loF4/80-cells. B cells, T cells, NK cells, and NKT cells were defined as SSC^lo CD45R+, SSC^loCD3ε+NK1.1-, SSC^loCD3ε+NK1.1+, and SSC^loCD3ε+NK1.1+, respectively. CD4 T cells (CD4+CD8-), CD8 T cells (CD4-CD8+), and regulatory T cells (CD4+CD8-CD25+) were also counted. Values represent the percentage of the non-parenchymal cells in each organ.

Yasuma T., Toda M., Abdel-Hamid A.M., D’Alessandro-Gabazza C., Kobayashi T., Nishihama K., D’Alessandro V.F., Pereira G.V., Mackie R.I., Gabazza E.C, & Cann I. (2021). Degradation Products of Complex Arabinoxylans by Bacteroides intestinalis Enhance the Host Immune Response. Microorganisms, 9(6), 1126.

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