Lapatinib ditosylate

Antibodies against AnxA6 as well as secondary anti-mouse, anti-goat, and anti-rabbit horseradish peroxidase-conjugated antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody against HIF-1α was purchased from BD Biosciences (Franklin Lakes, NJ, USA). Antibodies against HIF-2α and EGFR, were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibody against β-actin (ACTB) was purchased from Sigma Aldrich (St. Louis, MO, USA). Glucose uptake assay kit (Fluorometric; ab136956) and ATP Assay Kit (Colorimetric/Fluorometric; ab83355) were purchased from Abcam (Cambridge, UK). A set of EGFR-TKIs including lapatinib-ditosylate, erlotinib, gefitinib, and canertinib were purchased from BioVision Inc. (Milpitas, CA, USA). The AR antagonists bicalutamide and enzalutamide were purchased from Sigma Aldrich (St. Louis, MO, USA). Except otherwise indicated, all other reagents were purchased from Sigma Aldrich and/or Thermo Fisher Scientific (Waltham, MA, USA).

Antibodies and reagents were purchased as follows: mAb-2E9 (Abcam ab8465, RRID: AB_2096462), Anti-EGFR Affibody® Molecule (Abcam ab95116; RRID: AB_11156238), Anti-EGFR (D38B1, Cell Signaling Technology (CST) 4267; RRID: AB_2246311), Anti-Phospho-Akt (Ser473, D9E, CST 4060; RRID: AB_2315049), Anti-beta-Actin Monoclonal Antibody, HRP Conjugated (13E5 CST 5125; RRID: AB_1903890), Anti-Phospho-EGF Receptor (Tyr1068, D7A5 CST 3777; RRID: AB_2096270), Anti-EGFR (R & D Systems, AF231; RRID: AB_355220), Anti-EGFR (phospho Y992, EM-12, Abcam ab81440; RRID: AB_1658463), Anti-mouse IgG-HRP (Jackson ImmunoResearch 715-035-150; RRID: AB_2340770), Anti-rabbit IgG-HRP (Jackson ImmunoResearch 711-035-152; RRID: AB_10015282), Anti goat IgG-HRP (Jackson ImmunoResearch 705-035-147; RRID: AB_2313587), Anti-Rabbit-HRP (Dako P044801, RRID: AB_2617138), Recombinant Murine EGF (Peprotech, 315-09), Recombinant Murine IL-3 (Peprotech, 213-13), Erlotinib Hydrochloride (Biovision, 1558-100), Lapatinib Ditosylate (Biovision, 1642-25), 4% Paraformaldehyde (EM Grade, Electron Microscopy Service (EMS), 157-4), 25% Glutaraldehyde (Grade I, Sigma G5882), FluoSpheres™ (carboxylate modified, 0.1 μm, infrared (715/755), Invitrogen F8799).

Antibodies against AnxA6, as well as secondary anti-mouse, anti-goat, and anti-rabbit horseradish peroxidase-conjugated antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The antibodies against COXIV, Cytc. C, Pyruvate dehydrogenase (PDH), and Superoxide dismustase (SOD1) were purchased from Cell Signaling Technology (Beverly, MA, USA). Oil Red-O, Rotenone, 2-Deoxy-D-Glucose, Etomoxir, and antibody against β-actin (ACTB) were purchased from Sigma Aldrich (St. Louis, MO, USA). A set of EGFR-TKIs including lapatinib-ditosylate, erlotinib, gefitinib, and canertinib were purchased from BioVision (Milpitas, CA, USA). The Mito Stress Test Assay, Palmitate FAO Assay, and the culture media were purchased from Agilent Technologies (Santa Clara, CA, USA). Except where otherwise indicated, all the other reagents were purchased from Sigma Aldrich and/or Cell Signaling Technology.