Human il 1ra

Manufactured by Thermo Fisher Scientific

Sourced in United States

Human IL-1Ra is a recombinant protein that functions as an interleukin-1 receptor antagonist. It competitively binds to the interleukin-1 receptor, thereby inhibiting the proinflammatory effects of interleukin-1.

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Lab products found in correlation

Human il 1α, by Thermo Fisher Scientific (2 mentions) Mouse caspase 1, by R&D Systems (1 mentions) Human or murine il 1β elisa kits, by R&D Systems (1 mentions) Mouse il 1β, by R&D Systems (1 mentions) Nigericin sodium salt, by Merck Group (1 mentions) Lps from escherichia coli strain 0111 b4, by Merck Group (1 mentions) Lactacystin, by Santa Cruz Biotechnology (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Poly 1 c, by Merck Group (1 mentions) Human il 6, by R&D Systems (1 mentions) Hek blue il 1β cells, by InvivoGen (1 mentions) Hkb il1b, by InvivoGen (1 mentions) Human il 1β, by Thermo Fisher Scientific (1 mentions) Quanti blue assay, by InvivoGen (1 mentions) G csf, by R&D Systems (1 mentions) Il 1ra, by InvivoGen (1 mentions) Mrc 5 lung fibroblasts, by American Type Culture Collection (1 mentions) Murine il 4, by Thermo Fisher Scientific (1 mentions) Mouse igg1, by R&D Systems (1 mentions)

Close spelling variants of this product

Human IL-1RA ELISA Development Kit Recombinant human IL-1Ra IL-1RA

4 protocols using human il 1ra

Inflammatory Cytokine Quantification

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All chemicals used in the present study were purchased from Sigma (St. Louis, MO), unless otherwise noted. Ultrapure LPS, ATP, Caspase-1 inhibitor Ac-YVAD-cmk were purchased from Invivogen (San Diego, CA). Murine or human IL-1β, IL-1α and IL-18 from PeproTech (Rocky Hill, NJ); mouse IL1Ra from Novus Biotech, and human IL-1Ra from PeproTech. Human or murine IL-1β ELISA kits were from R&D system (Minneapolis, MN) or eBioscience (San Diego, California); Antibodies to human IL-1β, human caspase 1, mouse IL-1β and mouse caspase 1 were from R&D Systems or Santa Cruz Biotechnology (Santa Cruz, CA).

Guo B., Fu S., Zhang J., Liu B, & Li Z. (2016). Targeting inflammasome/IL-1 pathways for cancer immunotherapy. Scientific Reports, 6, 36107.

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Inflammasome Activation in Human Cells

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Human IL-1α, IL-1β, and IFNγ were purchased from Peprotech and used at 10 ng/ml unless indicated otherwise. IL-1α and IL-1β were used interchangeably with the same results. Human IL-1ra was purchased from Peprotech and was used at 1 µg/ml. Poly IC was purchased from Sigma and used at 10 µg/ml. LPS from Escherichia coli strain 0111:B4 was purchased from Sigma and was used at 100 ng/ml. Cells were treated for 6 h for Q-PCR and 24 h for ELISA, unless indicated otherwise. Cell treatment with inflammasome activators was performed following the published protocols [41] (link)–[43] (link). ATP (adenosine 5′-triphosphate disodium salt) was purchased from Sigma and was used at 5 mM. ATP was added to cultures 30 min before cell harvest. Nigericin sodium salt was purchased from Sigma and was used at 20 µM. Nigericin was added to culture 1 h before cell harvest. Lactacystin was purchased from Santa Cruz Biotechnology and was added to culture 10 min prior to cell stimulation.

Tarassishin L., Casper D, & Lee S.C. (2014). Aberrant Expression of Interleukin-1β and Inflammasome Activation in Human Malignant Gliomas. PLoS ONE, 9(7), e103432.

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Evaluating IL-1RA and Antibody Therapy in Cell-Based Assays

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40pg/uL human IL-1RA (PeproTech, US) was pre-incubated with 5ng/uL antibody (G4–21 or isotype control. IL-1RA and antibody were applied to HEK-Blue IL-1β cells (InvivoGen, US) for 2 hours and stimulated with 2pg/uL human IL-1β (PeproTech, US) for 36 hours at 37°C. Alternatively, cells were treated with 8–10 nM IL-1RA and commercial monoclonal (mAbs) or polyclonal antibodies (pAbs) at 40–2000nM, or with patient plasma at 1:10 and 1:20 dilutions, and subsequently stimulated with 0.1–1 nM IL-1β for 24 hours. Cells were treated with antibodies in the presence of IL-1α and IL-1RA, IL-1RA alone, or media, and supernatants assayed using the QUANTI-Blue assay (InvivoGen, hkb-il1b, US).
Human A549 lung epithelial cells and MRC-5 lung fibroblasts (ATCC, US) were treated with 10nM IL-1RA and patient plasma and stimulated with 0.5nM human IL-1α (PeproTech, US) for 24 hours at 37°C. RNA was isolated, cDNA prepared, and samples analyzed using TaqMan FAM-conjugated primer sets. Supernatants were collected and assayed for human IL-6 (R&D, US), IL-8 (R&D, US), and G-CSF (R&D, US) via ELISA.

Jarrell J.A., Baker M.C., Perugino C.A., Liu H., Bloom M.S., Maehara T., Wong H.H., Lanz T., Adamska J.Z., Kongpachith S., Sokolove J., Stone J.H., Pillai S.S, & Robinson W.H. (2021). Neutralizing Anti-Interleukin-1 Receptor-Antagonist Autoantibodies Induce Inflammatory and Fibrotic Mediators in IgG4-Related Disease. The Journal of allergy and clinical immunology, 149(1), 358-368.

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Antibody and Cytokine Assay Protocol

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Antibodies used for Western blotting, immunohistochemistry, immunofluorescence and fluorescent cell sorting are described in detail in Supplemental Table 1. The neutralizing antibody for mouse IL-13 (mabg-mil13) was from InvivoGen (San Diego, CA), and its matching isotype antibody (mouse IgG1) was from R&D Systems. Recombinant murine EGF, murine CCL2, human IL-1ra, murine IL-1β, murine IL-4, murine IL-10 and murine IL-13 were purchased from PeproTech (Rocky Hill, NJ). Hoechst 33342 was from Invitrogen. DAPI was from Sigma-Aldrich, and Matrigel was from BD Biosciences.

Liou G.Y., Bastea L., Fleming A., Döppler H., Edenfield B.H., Dawson D.W., Zhang L., Bardeesy N, & Storz P. (2017). Presence of Interleukin-13 at pancreatic ADM/PanIN lesions alters macrophage populations and mediates pancreatic tumorigenesis. Cell reports, 19(7), 1322-1333.

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