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Cd197 ccr7 clone g043h7 pe

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CD197/CCR7 (clone G043H7) PE is a monoclonal antibody that binds to the CCR7 (C-C chemokine receptor type 7) cell surface receptor. CCR7 is expressed on various immune cell types and plays a role in lymphocyte trafficking and homing.

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Lab products found in correlation

Cd45ra apc clone hi100, by BioLegend (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

CD197 (CCR7; G043H7; CD197 (clone G043H7; CCR7 (clone G043H7, CCR7 (G043H7, Clone G043H7 CCR7-PE CD197

2 protocols using cd197 ccr7 clone g043h7 pe

1

T Cell Subset Identification by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extracellular staining and acquisition were previously described.17 (link) The following antibodies were used: CD197/CCR7 (clone G043H7) PE (353203, BioLegend, San Diego, CA, USA), CD45RA (clone HI100) APC (304111, BioLegend, San Diego, CA, USA), and LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (L34966, Invitrogen, Carlsbad, CA, USA). The following gating strategy was used to assess different T cell subsets: Naïve Phenotype (CCR7+CD45RA+), Central Memory /TCM (CCR7+CD45RA−), Effector Memory Cells re-expressing CD45RA/TEMRA (CCR7−CD45RA+) and Effector Memory/TEM (CCR7−CD45RA−).
Cox M.J., Roman C.M., Tapper E.E., Siegler E.L., Chappell D., Durrant C., Ahmed O., Sinha S., Mwangi R., Scott N.S., Hefazi M., Schick K.J., Horvei P., Ruff M.W., Can I., Adada M., Bezerra E., Fonkoua L.A., Parikh S.A., Kay N.E., Sakemura R, & Kenderian S.S. (2022). GM-CSF disruption in CART cells modulates T cell activation and enhances CART cell anti-tumor activity. Leukemia, 36(6), 1635-1645.
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2

T Cell Subset Identification by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extracellular staining and acquisition were previously described.17 (link) The following antibodies were used: CD197/CCR7 (clone G043H7) PE (353203, BioLegend, San Diego, CA, USA), CD45RA (clone HI100) APC (304111, BioLegend, San Diego, CA, USA), and LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (L34966, Invitrogen, Carlsbad, CA, USA). The following gating strategy was used to assess different T cell subsets: Naïve Phenotype (CCR7+CD45RA+), Central Memory /TCM (CCR7+CD45RA−), Effector Memory Cells re-expressing CD45RA/TEMRA (CCR7−CD45RA+) and Effector Memory/TEM (CCR7−CD45RA−).
Cox M.J., Roman C.M., Tapper E.E., Siegler E.L., Chappell D., Durrant C., Ahmed O., Sinha S., Mwangi R., Scott N.S., Hefazi M., Schick K.J., Horvei P., Ruff M.W., Can I., Adada M., Bezerra E., Fonkoua L.A., Parikh S.A., Kay N.E., Sakemura R, & Kenderian S.S. (2022). GM-CSF disruption in CART cells modulates T cell activation and enhances CART cell anti-tumor activity. Leukemia, 36(6), 1635-1645.
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