Ohpak sb 804 hq column

Manufactured by Resonac

Sourced in Japan

The OHpak SB-804 HQ column is a laboratory equipment designed for high-performance liquid chromatography (HPLC) applications. The core function of this column is to separate and analyze complex mixtures of chemical compounds. The column utilizes a silica-based stationary phase to facilitate the separation process. No further details or interpretations about the intended use or performance capabilities of this product are provided.

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Lab products found in correlation

Ri 1530, by Jasco (3 mentions) Pu 980, by Jasco (2 mentions) Av 600 spectrometer, by Bruker (1 mentions) 5c18 ms 2 column, by Nacalai Tesque (1 mentions) Pu 2089 pump, by Jasco (1 mentions) Microtof q 3 spectrometer, by Bruker (1 mentions) Ri 2031 refractive index detector, by Jasco (1 mentions) Fp 6500 fluorometer, by Jasco (1 mentions) Co 2065 column oven, by Jasco (1 mentions) Agilent 1260 infinity system, by Agilent Technologies (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Bolt lds sample buffer, by Thermo Fisher Scientific (1 mentions) Bolt mops sds running buffer, by Thermo Fisher Scientific (1 mentions) Human serum albumin (hsa), by Fujifilm (1 mentions) Bolt reducing agent, by Thermo Fisher Scientific (1 mentions) N n diethyl p phenylenediamine, by Fujifilm (1 mentions) Precision plus protein dual color standard, by Bio-Rad (1 mentions) Mgcl2, by Fujifilm (1 mentions) Fecl3, by Fujifilm (1 mentions) Uv 2077, by Jasco (1 mentions)

Spelling variants of this product

SB-804 HQ (4 citations) OHpak SB-804 HQ (3 citations)

7 protocols using ohpak sb 804 hq column

DEAE-PEG and Catalase Conjugation

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DEAE-PEG (1000 μg, 480
nmol) and catalase
(50 μg, 0.2 nmol) were mixed in 180 μL of H₂O. The resulting mixture was incubated at 37 °C for 24 h, followed
by freeze-drying. Then, 250 μL of PBS(−) was added to
the dried sample. GFC was carried out using a JASCO PU-980 pumping
system (Tokyo, Japan) at a flow rate of 1.0 mL/min with a Shodex OHpak
SB-804 HQ column (Showa Denko K. K., Tokyo, Japan). The aqueous solution
containing PBS(−) was used as the mobile phase. The resulting
samples in PBS(−) (100μL) were injected into the column.
The eluate was detected with a RI detector (RI-1530; Jasco).

Asayama S., Nagashima K, & Kawakami H. (2017). Facile Method of Protein PEGylation by a Mono-Ion Complex. ACS Omega, 2(5), 2382-2386.

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Analytical Characterization of Compounds

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The NMR spectra were recorded using Bruker BioSpin AV-300 and AV-600 spectrometers. The ESI-MS spectra were recorded using a Bruker Daltonics micrOTOF Q-III spectrometer (Bruker Daltonics, Billerica, MA, USA). The HPLC and GPC measurements were conducted using a system consisting of a JASCO PU-2089 pump and a JASCO CO-2065 column oven (JASCO Corporation, Tokyo, Japan). A JASCO UV-2075 ultraviolet detector and a JASCO RI-2031 refractive index detector were used for the HPLC and GPC analyses, respectively. A 5C18-MS-II column (ɸ4.6 × 250 mm, Nacalai Tesque, INC.) was used for the HPLC analysis. 5, 4, 3, and 10 % MeCN-containing water were used as the eluents at a flow rate of 1.0 mL/min at 30 °C to analyze the enzymatic reaction with 1a – d , respectively. A Shodex OHpak SB-804 HQ column (ɸ8.0 × 300 mm, Showa Denko K.K., Tokyo, Japan) was used for the GPC analysis of 3a – c using a phosphate buffer (20 mM, pH 7.0) as the eluent at a flow rate of 0.5 mL/min at 30 °C. Pullulan samples were used as standards. A Shodex KD-804 column (ɸ8.0 × 300 mm, Showa Denko K.K.) was used for the GPC analysis of 3d using N , N -dimethylformamide (DMF) containing 10 mM lithium bromide as the eluent at a flow rate of 0.5 mL/min at 50 °C. Poly(methylmethacrylate) samples were used as standards. The fluorescence intensity was recorded using a JASCO FP-6500 fluorometer for the lectin binding tests.

Tanaka T., Matsuura A., Aso Y, & Ohara H. (2020). Aqueous One-pot Synthesis of Glycopolymers by Glycosidase-catalyzed Glycomonomer Synthesis Using 4,6-Dimetoxy Triazinyl Glycoside Followed by Radical Polymerization. Journal of Applied Glycoscience, 67(4), 119-127.

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Molecular Weight Determination by HPGPC

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Molecular weight was determined by high-performance gel permeation chromatography (HPGPC) on an Agilent 1260 Infinity system (Agilent, Palo Alto, CA, USA) installed with a refractive index detector and a Shodex OHpak SB-804HQ column (8.0 mm × 300 mm, Showa Denko, Tokyo, Japan). The sample solution (2 mg/mL) was filtered (0.22 µm) and injected into the column. The mobile phase was a 0.02% NaN₃ solution with a flow rate of 0.5 mL/min, and the column temperature was 35 °C. Linear regression was calibrated with a dextran series standard (5, 12, 50, 410, and 670 kDa).

Qiu S., Huang L., Xia N., Teng J., Wei B., Lin X, & Khan M.R. (2022). Two Polysaccharides from Liupao Tea Exert Beneficial Effects in Simulated Digestion and Fermentation Model In Vitro. Foods, 11(19), 2958.

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Investigating HDL Levels with ELISA Assay

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Fe(NH₄)₂(SO₄)₂, FeCl₃, N,N′-diethyl-p-phenylenediamine, dextran sulfate sodium salt (molecular weight range, 36,000–50,000), MgCl₂, and human serum albumin were purchased from Wako (Tokyo, Japan). Tert-butyl hydroperoxide (70% in water) was purchased from TCI (Tokyo, Japan). NaCl was from Sigma-Aldrich (Tokyo, Japan). HDL Human ELISA (enzyme-linked immunosorbent assay) Kit (ab125961) came from Abcam (Cambridge, UK). A Bolt LDS sample Buffer, Bolt Reducing Agent, and Bolt MOPS SDS Running Buffer were purchased from the Life Technologies Corporation (Carlsbad, CA, USA). The Precision Plus Protein Dual Color Standards were from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). The OH pak SB-804HQ column was obtained from Showa Denko KK (Tokyo, Japan).

Ito F., Ito T., Suzuki C., Yahata T., Ikeda K, & Hamaoka K. (2017). The Application of a Modified d-ROMs Test for Measurement of Oxidative Stress and Oxidized High-Density Lipoprotein. International Journal of Molecular Sciences, 18(2), 454.

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Gel Filtration Chromatography Protocol

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GFC was performed by a JASCO PU-980 pumping system (Tokyo, Japan) with a Shodex OHpak SB-804 HQ column (Showa Denko K. K., Tokyo, Japan) at a flow rate of 1.0 mL/min. The mobile phase containing CH₃COOH (0.5 M) and NaNO₃ (0.2 M) was used. In the column, 100 μL of 1 mg/mL samples were injected. The eluate was detected with an RI detector (RI-1530; JASCO, Tokyo, Japan). Polyethylene glycol standards were used for calibration.

Endo A, & Asayama S. (2021). Hepatocyte-Specific Co-Delivery of Zinc Ions and Plasmid DNA by Lactosylated Poly(1-vinylimidazole) for Suppression of Insulin Receptor Internalization. Pharmaceutics, 13(12), 2084.

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Gel Filtration Chromatography of Protein Samples

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GFC was carried
out using a JASCO PU-980 pumping system (Tokyo, Japan) at the flow
rate of 1.0 mL/min with a Shodex OHpak SB-804 HQ column (Showa Denko
K. K., Tokyo, Japan). The aqueous solution containing PBS(-) was used
as a mobile phase. One hundred microliters of 1 mg/mL samples, which
were incubated at 37 °C for 2 weeks in (4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid) buffer at pH 7.4 or pH 5.0, were injected into the column. The
eluate was detected with a RI detector (RI-1530; Jasco) and a UV detector
(UV-2077; Jasco).

Kobayashi Y., Taneichi S., Kawakami H., Negishi Y, & Asayama S. (2019). Plasmid DNA Mono-Ion Complex for in Vivo Sustainable Gene Expression. ACS Omega, 4(7), 11464-11471.

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SCRP Molecular Weight Distribution

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The molecular weight distribution of the SCRP sample was determined on a Shimadzu LC-20AT HPLC system (Shimadzu Corp., Tokyo, Japan) equipped with a RID-10A refractive index detector (Shimadzu Corp., Tokyo, Japan) and a Shodex Ohpak SB-804HQ column (8.0 mm × 300 mm, 10 μm, Showa Denko, Tokyo, Japan) placed in a 30°C column oven (Sun et al., 2018) (link). The injection sample was 20 μL of the SCRP solution (10 mg/mL). The elution phase was ultrapure water at a flow rate of 0.8 mL/min. Seven dextran standards (Nanjing Dichun Biotechnology Co., Ltd., Nanjing, China) with different Zhang RC et al. mL and incubated at 37°C in 95% air and 5% CO 2 for 24 h. Then, 10 μL of SCRP solutions with different concentrations of 0 (Control), 50 (low concentration SCRP, LSCRP), 100 (middle concentration SCRP, MSCRP) and 200 µg/mL (high concentration SCRP, HSCRP) were added into 96-well plates and incubated for another 24 h. Cell morphology was observed on an inverted microscope (×200).

Zhang R., Liu R., Chen Y., Wu T., Sui W., & Zhang M. (2020). Physicochemical and biological activities of polysaccharides from the waste residue of sea cucumber peptide production. Quality Assurance and Safety of Crops & Foods, 12(4), 1-14.

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