Nf κb inhibitor sc75741

Bladder cancer SW780 and T24 cells were cultured in DMEM medium containing 10% (v/v) fetal bovine serum and 1% penicillin-streptomycin (Life technology, United States) at 37°C in 5% CO₂ humidified incubator. MTT and Doxrubicine (DOX) were obtained from Sigma, USA. Transwell plates were from Corning Incorporated, United States. Annexin V-FITC kit was purchased from BD Pharmingen, United States. NF-κB, NF-κB p65 (p-NF-κB), MDM2, Phospho-MDM2 (p-MDM2), p53, p21, Bcl-2, and PARP were purchased from Cell signaling technology, United States. Alanine transaminase (ALT), Creatine kinase (CK), and aspartate transaminase (AST) kits were obtained from Stanbio, United States. NF-κB inhibitor (SC75741) was purchased from Selleck, United States.

Phorbol-12-myristate-13-acetate (PMA), gelatin, Triton X-100, Coomassie brilliant blue R-250 was purchased from Sigma-Aldrich, MMP-9 inhibitor (SB-3CT) and NF-κB inhibitor (sc-75741) were purchased from Selleck. Recombinant human MMP-9 protein and Recombinant mice MMP-9 protein were purchased from R&D systems. Recombinant human pro-MMP-9 protein were purchased from ProSpec. Commercialized DENV2-NS1 protein were purchased from Native Antigen. Trizol reagent was purchased from Ambion. Lipofectamine 2000 reagent was purchased from Invitrogen. Human MMP-9 ELISA kit was purchased from BD Biosciences. Membrane maker Dil (D8700) was purchased from Solarbio. NF-κB Pathway Sampler Kit (#9936T), Tight Junction Antibody Sampler Kit (#8683T), Cadherin-Catenin Antibody Sampler Kit (#9961T), and Antibodies against TIMP-1 (8946S) were purchased from Cell Signaling Technology. Antibody against DENV-NS3 (GTX124252) were purchased from Genetex. Antibodies against DENV2-NS1 were purchased from Arigo biolaboratories (SQab1501). Antibodies against Flag (F3165) and HA (H6908) were purchased from Sigma. Anti-β-actin antibody (66009) were purchased from Proteintech. Rabbit IgG and Mouse IgG were purchased from Invitrogen. Anti-Mouse IgG Dylight 649, Anti-Mouse IgG Dylight 488, Anti-Rabbit IgG Dylight 649, and Anti-Rabbit IgG FITC were purchased from Abbkine.