P34 arc arpc2

The following primary antibodies were used: Trio (DeGeer et al., 2013 (link)), Western blot (WB), 1/1000; immunofluorescence (IF), 1/100; MgcRacGAP (Abcam, Paris, France; WB, 1/250; IF, 1/2000); α-tubulin (DM1alpha; Sigma-Aldrich, St. Louis, MO; WB, 1/400; IF, 1/200), fluorescein isothiocyanate–conjugated α-tubulin (DM1alpha; Sigma-Aldrich; IF, 1/500), Rac1 (BD Biosciences, Franklin Lakes, NJ; WB, 1/2000), rhodamine-phalloidin (Sigma-Aldrich; IF, 1/10,000), BCR (Cell Signaling, Danvers, MA; WB, 1/1000), β-Pix (gift from N. Morin; CRBM–CNRS, Montpellier, France; WB, 1/5000), GFP (Life Technologies; WB, 1/5000), p34-Arc/ARPC2 (Millipore, Molsheim, France; WB 1/5000), Phospho Histone H3 (Millipore; WB 1/5000), Ect2 (Santa Cruz C-20, Santa Cruz, CA; WB 1/1000), and DAPI (Cell Signaling; IF, 1/10 000). Secondary antibodies include, for IF, Alexa Fluor 488, 568, or 647–conjugated anti-rabbit or anti-mouse secondary antibodies (Molecular Probes, Carlsbad, CA; 1/1500) or 555–conjugated anti-goat (Jackson Immunoresearch, West Grove, PA; 1/500), and for WB, anti-mouse and anti-rabbit immunoglobulin G (IgG), horseradish peroxidase (HRP)–linked antibody (Cell Signaling; 1/2000) and anti-goat IgG, HRP-linked antibody (Jackson ImmunoResearch; 1/2000).