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Matchmaker gal4 two hybrid

Manufactured by Takara Bio
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The Matchmaker GAL4 Two-Hybrid System is a tool for studying protein-protein interactions. It utilizes the GAL4 transcription factor from Saccharomyces cerevisiae to detect and analyze interactions between two proteins of interest.

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Lab products found in correlation

Yeast strain ah109, by Takara Bio (2 mentions) Yeast transformation kit, by Takara Bio (2 mentions)

Close spelling variants of this product

Matchmaker GAL4 Two‐Hybrid System 3 kit Matchmaker GAL4 Yeast Two-Hybrid System 3 Matchmaker GAL4 Two-Hybrid System Matchmaker GAL4 Two‐Hybrid System 2 Matchmaker™ GAL4 Two-Hybrid System 3 & Libraries Matchmaker GAL4 Two-Hybrid System 3 & Libraries User Manual Matchmaker GAL4 yeast two-hybrid system Matchmaker GAL4-based two-hybrid system 3 Matchmaker GAL4 Two-Hybrid System 3 Matchmaker GAL4-based yeast two-hybrid system

2 protocols using matchmaker gal4 two hybrid

1

Yeast Two-Hybrid DLP1 and VPS35 Interaction

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Yeast two hybrid experiments were performed using the Matchmaker GAL4 Two-Hybrid (Clontech) System 3 with vectors pGBKT7 and pGADT7 containing the binding domain and activation domain respectively. Yeast strain AH109 (Clontech) was transformed with empty pGBKT7 and pGADT7 vectors or pGBKT7 and pGADT7 vectors encoding DLP1 or VPS35 using Yeast Transformation Kit (Clontech). After transformation, yeast cells were plated on SD/-Leu/-Trp plates followed by streaked onto high-stringency plates (SD/-Leu/-Trp/-His/-Ade). β-Galactosidase activity in different yeast clones was measured by Yeast beta-Galactosidase Assay Kit (Piercenet) following Manufacturer’s instructions.
Wang W., Wang X., Fujioka H., Hoppel C., Whone A.L., Caldwell M.A., Cullen P.J., Liu J, & Zhu X. (2015). Parkinson’s disease-associated mutant VPS35 causes mitochondrial dysfunction by recycling DLP1 complexes. Nature medicine, 22(1), 54-63.
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2

Yeast Two-Hybrid DLP1 and VPS35 Interaction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Yeast two hybrid experiments were performed using the Matchmaker GAL4 Two-Hybrid (Clontech) System 3 with vectors pGBKT7 and pGADT7 containing the binding domain and activation domain respectively. Yeast strain AH109 (Clontech) was transformed with empty pGBKT7 and pGADT7 vectors or pGBKT7 and pGADT7 vectors encoding DLP1 or VPS35 using Yeast Transformation Kit (Clontech). After transformation, yeast cells were plated on SD/-Leu/-Trp plates followed by streaked onto high-stringency plates (SD/-Leu/-Trp/-His/-Ade). β-Galactosidase activity in different yeast clones was measured by Yeast beta-Galactosidase Assay Kit (Piercenet) following Manufacturer’s instructions.
Wang W., Wang X., Fujioka H., Hoppel C., Whone A.L., Caldwell M.A., Cullen P.J., Liu J, & Zhu X. (2015). Parkinson’s disease-associated mutant VPS35 causes mitochondrial dysfunction by recycling DLP1 complexes. Nature medicine, 22(1), 54-63.
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