Dulbecco s modified eagle minimal essential medium

Manufactured by Thermo Fisher Scientific

Dulbecco's modified Eagle minimal essential medium (DMEM) is a cell culture medium formulation that supports the growth and maintenance of various cell types. It provides essential nutrients, vitamins, and amino acids required for cell proliferation. The medium is commonly used in research and biopharmaceutical applications.

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Lab products found in correlation

H9 wa09, by WiCell (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Accutase, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Sk n as neuroblastoma cells, by American Type Culture Collection (1 mentions)

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2 protocols using dulbecco s modified eagle minimal essential medium

Maintaining Human Embryonic Stem Cells and Mouse Fetal Liver Cells

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H9 (WA09; WiCell) human ES cells were maintained using Pluripro fully defined media and matrix (Cell Guidance Systems). Approximately 50 million cells (at passage 56) were harvested with Accutase (Life Technologies), suspended in Pluripro media and directly processed for fixation.
Mouse foetal livers were dissected from C57BL/6 mouse embryos at day 14.5 (E14.5) of development. Foetal liver cells were suspended in DMEM (Dulbecco's modified Eagle minimal essential medium; Life Technologies) supplemented with 10 % foetal bovine serum, filtered through a cell strainer (70 μm) and directly fixed by addition of formaldehyde.

Nagano T., Várnai C., Schoenfelder S., Javierre B.M., Wingett S.W, & Fraser P. (2015). Comparison of Hi-C results using in-solution versus in-nucleus ligation. Genome Biology, 16(1), 175.

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Culturing SK-N-AS Neuroblastoma Cells

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Human, SK‐N‐AS neuroblastoma cells were obtained from American Type Culture Collection (Rockville, MD). They were cultured in Dulbecco's modified Eagle minimal essential medium (Life Technologies, Grand Island, NY) containing 10% fetal bovine serum (product number A316040; Gibco, Gaithersburg, MD) and 1% nonessential amino acids (Invitrogen) at 37°C under 5% CO₂.

Kuzyk C.L., Anderson C.C, & Roede J.R. (2020). Simvastatin Induces Delayed Apoptosis Through Disruption of Glycolysis and Mitochondrial Impairment in Neuroblastoma Cells. Clinical and Translational Science, 13(3), 563-572.

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