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Dichloromethane dcm

Manufactured by Daejung Chemicals

Dichloromethane (DCM) is a colorless, volatile liquid commonly used as a solvent in various laboratory applications. It has a low boiling point and is miscible with a wide range of organic solvents. Dichloromethane serves as a core functional solvent for extraction, purification, and cleaning processes in a laboratory setting.

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6 protocols using dichloromethane dcm

1

Solid-Phase Peptide Synthesis Protocol

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2-Chlorotrityl chloride
(CTC) (100–200 mesh, 1.34 mmol/g) resin, fritted polypropylene
tubes (Libra tube RT-20M, 20 mL), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium
hexafluorophosphate (HBTU), hydroxybenzotriazole (HOBt), and Fmoc-protected
amino acids were purchased from BeadTech Inc. (Seoul, Korea). N,N-Diisopropylethylamine (DIPEA), phenol,
thioanisole, and 1,2-ethanedithiol (EDT) were purchased from Aldrich
(St. Louis, MO, USA). Dichloromethane (DCM), N,N-dimethylformamide (DMF), methanol, piperidine, diethyl
ether, and trifluoroacetic acid (TFA) were obtained from Dae-Jung
Chemicals (Korea). Hydrogen peroxide solution (30%), sulfuric acid
(98%), DMSO, and CuCl2 were of analytical grade and used
as purchased.
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2

Electrochemical Sensing with Aromatic Monomers

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Aromatic monomers, such as anthracene (>99%),
pyrene (98%) and naphthalene (>98%; TCI, Tokyo, Japan), and glucose,
alizarin red S, dopamine, potassium ferricyanide(III), and Nafion
117 solution (∼5% in a mixture of lower aliphatic alcohols
and water) were obtained from Sigma-Aldrich and used without further
purifications. Other chemicals such as APBA (97%) and triethyl amine
(TEA, 99%) (from Alfa Aesar, Incheon, South Korea), BME (95%) cross-linker
(TCI), and ZnBr2 (98%) (Thermo Fisher Scientific, South
Korea) were also used as received. Potassium chloride, the PBS containing
disodium hydrogen phosphate (Na2HPO4) and sodium
dihydrogen phosphate (NaH2PO4), DCE, and dichloromethane
(DCM) were obtained from Daejung Chemical Co. (Korea), and DCE and
DCM were distilled before use.
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3

Biocompatible Hydrogel Formulation

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Injectable crosslinked hyaluronic acid-based hydrogel (HA gel) was obtained from CHA Meditech Co., Ltd. (Gyeonggi-do, Korea). Poly(D, L-lactide-co-glycolide) (PLGA, LA:GA = 50:50, MW 40,000 Da) was obtained from Evonik Ind. (Essen, Germany). Magnesium hydroxide (Mg(OH)2, MH), hyaluronidase, and phosphate-buffered saline (PBS, Gibco, Franklin Lakes, NJ) were purchased from Sigma-Aldrich (St. Louis, MO). Dichloromethane (DCM) was purchased from Daejung Chemical (Gyeonggi-do, Korea).
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4

Nanoparticle-Mediated Taxane Delivery

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The PS-PDLLA was purchased from PolySciTech, a division of Akina, Inc. (West Lafayette, IN, USA). Taxotere was obtained from Sanofi S.A. Docetaxel (DCT) was acquired from LC Laboratories (Woburn, MA, USA). Paclitaxel (PTX) was purchased from Samyang Genex Corporation (Daejeon, Korea). Tween 80 was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Phosphate-buffered saline (PBS) was purchased from Biosesang (Seongnam, Korea). Dichloromethane (DCM) and dimethyl sulfoxide (DMSO) were purchased from Daejung Chemicals & Metals Co., Ltd. (Seoul, Korea). Poly(vinyl alcohol) (PVA), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) solution, and sodium bicarbonate (NaHCO3) solution were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). RPMI 1640 media, penicillin, streptomycin, and fetal bovine serum (FBS) were purchased from Gibco Life Technologies, Inc. (Thermo Fisher Scientific, Waltham, MA, USA). All other reagents were of analytical grade and were acquired from commercial sources.
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5

PLGA-Based Nanoparticle Formulation

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Poly (lactic-co-glycolic acid) (PLGA; lactic acid:glycolic acid = 1:1, viscosity 0.55–0.75 DL/g, Part#: B6013-2P was purchased from Durect Corporation (AL, USA). Poly(vinyl alcohol) (PVA), Tris buffer (pH 8.5) and polydopamine (PD) were obtained from Sigma-Aldrich (PA, USA). Poly (ethylene glycol) (PEG, 4 arm-amine termini, HCL salt) was provided by JenKem, (TX, USA). Dichloromethane (DCM) and DNase-1 were purchased from Daejung (Seoul, South Korea) and Roche (Basel, Switzerland), respectively.
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6

Doxorubicin and Erlotinib Nanoparticle Formulation

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Doxorubicin hydrochloride salt (DOX) and erlotinib free base (ERL) were obtained from LC Laboratories (Woburn, MA, USA). Rhamnolipid (RL) was purchased from AGAE Technologies (Corvallis, OR, USA). Poly(vinyl alcohol) (PVA), poly(d,l-lactide-co-glycolide) (PLGA), pluronic F-127, Tween 80, and sodium chloride (NaCl) were purchased from Sigma-Aldrich (Logan, UT, USA). Dimethyl sulfoxide (DMSO) was purchased form Samchun (Pyeongtaek, Gyeonggi-do, Korea). Dichloromethane (DCM) and Triton X-100 were obtained from Daejung Chemical Co. (Siheung, Gyeonggi-do, Korea). Glycerin was purchased from Junsei Chemical Co., Ltd (Tokyo, Japan). DiIC18(5) solid (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt) (DiD) and Hoechst 33,342 were obtained from Invitrogen (Waltham, MA, USA). RPMI medium, Dulbecco’s phosphate-buffered saline (DPBS), and fetal bovine serum (FBS) were obtained from Biowest (Nuaille, France). Thiazolyl blue tetrazolium bromide (3-[4,5-dimethyl-thiazol-2-yl]2,5-diphenyltetrazolium bromide) (MTT), phosphate-buffered saline (PBS), 4% paraformaldehyde, and 10% formalin were obtained from Biosesang (Seongnam, Gyeonggi-do, Korea). Antibiotic-antimycotic solution and 0.05% Trypsin-EDTA were purchased from Gibco-BRL (Grand Island, NY, USA). Optimal cutting temperature (OCT) compound was purchased from Scigen Scientific, Inc. (Gardena, CA, USA).
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