Doxorubicin hydrochloride salt (DOX) and erlotinib free base (ERL) were obtained from LC Laboratories (Woburn, MA, USA). Rhamnolipid (RL) was purchased from AGAE Technologies (Corvallis, OR, USA). Poly(vinyl alcohol) (PVA), poly(
d,
l-lactide-
co-glycolide) (PLGA), pluronic F-127,
Tween 80, and sodium chloride (NaCl) were purchased from Sigma-Aldrich (Logan, UT, USA).
Dimethyl sulfoxide (DMSO) was purchased form Samchun (Pyeongtaek, Gyeonggi-do, Korea).
Dichloromethane (DCM) and
Triton X-100 were obtained from Daejung Chemical Co. (Siheung, Gyeonggi-do, Korea).
Glycerin was purchased from Junsei Chemical Co., Ltd (Tokyo, Japan). DiIC
18(5) solid (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt) (DiD) and
Hoechst 33,342 were obtained from Invitrogen (Waltham, MA, USA).
RPMI medium,
Dulbecco’s phosphate-buffered saline (DPBS), and
fetal bovine serum (FBS) were obtained from Biowest (Nuaille, France). Thiazolyl blue tetrazolium bromide (3-[4,5-dimethyl-thiazol-2-yl]2,5-diphenyltetrazolium bromide) (MTT), phosphate-buffered saline (PBS), 4% paraformaldehyde, and 10% formalin were obtained from Biosesang (Seongnam, Gyeonggi-do, Korea).
Antibiotic-antimycotic solution and
0.05% Trypsin-EDTA were purchased from Gibco-BRL (Grand Island, NY, USA). Optimal cutting temperature (OCT) compound was purchased from Scigen Scientific, Inc. (Gardena, CA, USA).
Lee Y., Lee D., Park E., Jang S.Y., Cheon S.Y., Han S, & Koo H. (2021). Rhamnolipid-coated W/O/W double emulsion nanoparticles for efficient delivery of doxorubicin/erlotinib and combination chemotherapy. Journal of Nanobiotechnology, 19, 411.