For the transwell migration assays, 10×104 cells were plated in the top chamber with a non-coated membrane (24-well insert; 8 mm pore size; BD Biosciences). For the invasion assays, 2×105 cells were plated in the top chamber with a Matrigel-coated membrane (24-well insert; 8 mm pore size; BD Biosciences). For both assays, the cells were plated in a serum-free medium, and a medium supplemented with 10% serum was used as a chemoattractant in the lower chamber. The cells were incubated for 16 h at 37°C and 5% CO2 in a tissue culture incubator. After 16 h, the non-migrated/non-invading cells were removed from the upper sides of the transwell membrane filter inserts using cotton-tipped swabs. The migrated/invaded cells on the lower sides of the inserts were stained with Giemsa, and the cells were counted.
Matrigel coated membrane 24 well insert 8 mm pore size
Manufactured by BD
Matrigel-coated membrane (24-well insert; 8 mm pore size) is a laboratory equipment used for cell culture applications. It consists of a 24-well insert with a membrane that has been coated with Matrigel, a basement membrane extract. The membrane has a pore size of 8 mm.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using matrigel coated membrane 24 well insert 8 mm pore size
1
Transwell Migration and Invasion Assays
2
Transwell Assay for Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the Transwell migration assays, 1×104 cells were plated in the top chamber with a non-coated membrane (24-well insert; 8 μm pore size; BD Biosciences). For the invasion assays, 2×105 cells were plated in the top chamber with a Matrigel-coated membrane (24-well insert; 8 mm pore size; BD Biosciences). For both assays, the cells were plated in a serum-free medium, and medium supplemented with 10% serum was used as a chemoattractant in the lower chamber. The cells were incubated for 16 h at 37°C and 5% CO2 in a tissue culture incubator. After 16 h, the non-migrated/non-invading cells were removed from the upper sides of the Transwell membrane filter inserts using cotton-tipped swabs. The migrated/invaded cells on the lower sides of the inserts were stained with Giemsa, and the cells were counted.
3
Transwell Migration and Invasion Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the Transwell migration assays, 1×104 cells were plated in the top chamber with a non-coated membrane (24-well insert; 8-mm pore size; BD Biosciences, Franklin Lakes, NJ, USA). For the invasion assays, 2×105 cells were plated in the top chamber with a Matrigel-coated membrane (24-well insert; 8 mm pore size; BD Biosciences). For the two assays, the cells were plated in a serum-free medium, and medium supplemented with 10% serum was used as a chemoattractant in the lower chamber. The cells were incubated for 16 h at 37°C and 5% CO2 in a tissue culture incubator. After 16 h, the non-migrated/non-invading cells were removed from the upper sides of the Transwell membrane filter inserts using cotton-tipped swabs. The migrated/invaded cells on the lower sides of the inserts were stained with Giemsa, and the cells were counted.
4
Transwell Assays for Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In the transwell migration assays and the invasion assays, the cells were first placed in serum-free medium, and the medium supplemented with 10% serum was placed in the lower chamber as a chemoattractant. The former was seeded with 1 × 104 cells in an upper chamber with a non-coated membrane (24-well insert; 8 mm pore size; BD Biosciences), and the latter seeded with 2 × 105 cells with a Matrigel-coated membrane (24-well insert; 8 mm pore size; BD Biosciences). The cells were incubated in a tissue culture incubator at 37 °C and 5% CO 2 for 16 h, after which the ummigrated/non-invasive cells on the upper sides of the Transwell membrane filter insert were gently wiped off with a cotton swab. On the underside of the insert, cells were stained with crystal violet and counted.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!