Cx3cr1 gfp b6.129p cx3cr1tm1litt j mice

Manufactured by Jackson ImmunoResearch

CX3CR1-GFP (B6.129P-Cx3cr1tm1Litt/J) mice are a genetically modified mouse strain that expresses green fluorescent protein (GFP) under the control of the Cx3cr1 gene promoter. The Cx3cr1 gene encodes the CX3C chemokine receptor 1, which is involved in the migration and function of certain immune cells. This mouse strain allows for the visualization and study of the CX3CR1-expressing cells, such as monocytes, macrophages, and microglia, in various biological processes and disease models.

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C57bl 6 mice, by Jackson ImmunoResearch (2 mentions)

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CX3CR1-GFP (49 citations) CX3CR1-GFP mice (37 citations) B6.129P-Cx3cr1tm1Litt/J (21 citations) CX3CR1−/− mice (5 citations) B6.129P-Cx3cr1tm1Litt/J mice (4 citations)

2 protocols using cx3cr1 gfp b6.129p cx3cr1tm1litt j mice

Histology, PCR, and Behavioral Assays in C57BL/6 Mice

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For histology, real-time polymerase chain reaction (PCR), and behavioral assays, 6-wk-old, male C57BL/6 mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA). Additionally, male CX3CR1-GFP (B6.129P-Cx3cr1tm1Litt/J) mice were also obtained from Jackson Laboratory to analyze glial activation in the NAc. All animals were housed on a 12-h light–dark cycle with unlimited access to water and standard calorie chow. After arriving at the University Laboratory Animal Research (ULAR) facility, animals were group housed for the first 48 h, then separated and housed individually for 24 h prior to the induction of CCI injury. The Institutional Animal Care and Use Committee at Temple University (Philadelphia, PA, USA) approved all procedures involving the use of vertebrate animals.

Merkel S.F., Andrews A.M., Lutton E.M., Razmpour R., Cannella L.A, & Ramirez S.H. (2017). Dexamethasone Attenuates the Enhanced Rewarding Effects of Cocaine Following Experimental Traumatic Brain Injury. Cell Transplantation, 26(7), 1178-1192.

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Traumatic Brain Injury in C57BL/6 Mice

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For histology, real-time PCR, and behavioral assays, six-week old, male C57BL/6 mice were obtained from Jackson Laboratory (Bar Harbor, ME). Additionally, male CX3CR1-GFP (B6.129P-Cx3cr1tm1Litt/J) mice were also obtained from Jackson Laboratory to analyze glial activation in the nucleus accumbens. All animals were housed on a 12-hour light-dark cycle with unlimited access to water and standard calorie chow. After arriving at the University Laboratory Animal Research (ULAR) facility, animals were group housed for the first 48 hours, then separated and housed individually for 24 hours prior to the induction of controlled cortical impact injury. The Institutional Animal Care and Use Committee (IACUC) at Temple University (Philadelphia, PA) approved all procedures involving the use of vertebrate animals.

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