The original IsoFlux™ CTC enrichment protocol was modified to enable the replacement of IsoFlux™ beads with CELLection™ Dynabeads (Invitrogen). CELLection™ Dynabeads® coated with a human anti-mouse IgG (Invitrogen) were utilized for enrichment of the cells. Dynabeads were incubated with EPCAM (Ber-EP4, Abcam) antibody (0.02 µg antibody/µl bead suspension) at room temperature. After incubation, washed the beads with PBS with 0.1% bovine serum albumin (BSA) twice and stored the beads at 4 °C.
Cellection dynabeads
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CELLection Dynabeads are a versatile magnetic bead technology that can be used for the isolation, separation, and enrichment of a variety of cell types. The beads are coated with specific antibodies or ligands that bind to target cells, allowing for their efficient capture and isolation from complex samples.
Automatically generated - may contain errors
4 protocols using cellection dynabeads
1
Enrichment of Circulating Tumor Cells using CELLection Dynabeads
2
CTC Enrichment with Cleavable DNA Linker
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We modified the CTC enrichment protocol provided by IsoFluxTM, which makes replacement of IsoFluxTM beads with CELLection™ Dynabeads (Invitrogen, Waltham, MA, USA) possible. Antibodies are attached to the Dynabeads via a DNA linker. The DNA linker provides a cleavable site to remove the beads from the cells after the enrichment process, which would otherwise not be feasible for the original beads provided by IsoFlux. Further molecular analyses could be performed using separated, ready-for-use cancer cells following immediate access to high-quality, viable cells in a high-density format. CELLection™ Dynabeads® coated with human anti-mouse IgG (Invitrogen) were used for cell isolation. Dynabeads were incubated with EPCAM antibody (Ber-EP4, Abcam, Cambridge, UK; 0.02 µg antibody/µL bead suspension) at room temperature. The beads were stored at 4 °C after being washed with phosphate-buffered saline (PBS) with 0.1% bovine serum albumin (BSA) twice.
3
Antibody-Coated Dynabead Enrichment for CTC Isolation
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The original IsoFlux CTC enrichment protocol was modified to enable the replacement of IsoFlux beads with CELLection Dynabeads (Invitrogen). Antibodies are attached to the surface of the Dynabeads via a DNA linker, which provides a cleavable site to release and remove the beads from the cells after isolation. This process is not possible with the IsoFlux beads. CELLection Dynabeads coated with human antimouse immunoglobulin (Ig) G (Invitrogen) was utilized for cell enrichment. Dynabeads were incubated with each antibody (0.02 µg antibody/ µL bead suspension) (EpCAM [Ber-EP4], Cambridge, United Kingdom) at room temperature. After incubation, the beads were washed twice with phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) and stored at 4°C.
4
Isolation and Culture of Murine Retinal Ganglion Cells
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