The standards of 8-iso-PGF2α and its deuterated analogue (8-iso-PGF2α-d4) were purchased from Cayman Chemicals (Ann Arbor, MI, USA); LC-MS-grade methanol (MeOH) was delivered by VWR Chemicals BDH (Avantor, USA); 98% formic acid, ethyl ethanoate, hexane, methylene chloride, and sodium phosphate monobasic were obtained from Sigma-Aldrich (St. Louis, MI, USA). Stock solutions of 8-iso-PGF2α (1.0 mg/L), of 8-iso-PGF2α-d4 (1.0 mg/L) and working internal standard solution of 8iso-PGF2α-d4 (10 mg/L) were prepared in 50% MeOH. From separately prepared stocks, working solutions of 8-iso-PGF2α were prepared in 50% MeOH with concentrations of 10, 16, 20, 50, 100, 200 and 500 µg/L for the preparation of the calibration curve (CC) samples, and with concentrations of 10, 25, 250, and 400 µg/L for the preparation of the lower limit of quantification (LLOQ) and quality control (QC) samples at three levels.
Lc ms grade methanol meoh
Manufactured by Avantor
Sourced in United States
Avantor's LC-MS grade methanol (MeOH) is a high-purity solvent designed for use in liquid chromatography-mass spectrometry (LC-MS) applications. It meets the stringent requirements for analytical and research-grade use.
Automatically generated - may contain errors
Lab products found in correlation
Hexane, by Merck Group (1 mentions)
Methylene chloride, by Merck Group (1 mentions)
8 iso pgf2α, by Cayman Chemical (1 mentions)
Sodium phosphate monobasic, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
8 iso pgf2α d4, by Cayman Chemical (1 mentions)
Methyl tertiary butyl ether, by Merck Group (1 mentions)
Sulfuric acid, by Avantor (1 mentions)
Sodium bicarbonate nahco3, by Merck Group (1 mentions)
Sodium sulfate na2so4, by Merck Group (1 mentions)
Apigenin, by Merck Group (1 mentions)
Chlorbromuron, by LGC (1 mentions)
Oasis prime hlb cartridge, by Waters Corporation (1 mentions)
Formic acid, by Avantor (1 mentions)
Acetic acid, by Avantor (1 mentions)
Sodium chloride (nacl), by ITW Reagents (1 mentions)
Ultrapure water system, by Sartorius (1 mentions)
4 mbp, by Merck Group (1 mentions)
Sepra c18 e, by Phenomenex (1 mentions)
Quercetin, by Merck Group (1 mentions)
5 protocols using lc ms grade methanol meoh
1
Quantification of Urinary 8-iso-PGF2α
2
Quantification of Halogenated Disinfection Byproducts
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LC-MS grade methanol (MeOH) and sulfuric acid 95–97% Merck were acquired from VWR (Spånga, Sweden). Methyl tertiary butyl ether (MTBE) 98%, sodium sulfate (Na2SO4), and sodium bicarbonate (NaHCO3) were acquired from Sigma-Aldrich (Stockholm, Sweden) and ethanol 96% from Solveco (Rosersberg, Sweden). The selected compounds including their class, chemical name, and abbreviation are presented in Table 1 .
Summary of selected compounds along with their class, compound name, and abbreviation
| Compound class | Compound name | Abbreviation |
|---|---|---|
| Trihalomethanes (THMs) | Tribromomethane (bromoform) | TBM |
| Trichloromethane (chloroform) | TCM | |
| Bromodichloromethane | BDCM | |
| Dibromochloromethane | DBCM | |
| Haloacetonitriles (HANs) | Bromochloroacetonitrile | BCAN |
| Dibromoacetonitrile | DBAN | |
| Dichloroacetonitrile | DCAN | |
| Trichloroacetonitrile | TCAN | |
| Haloketones (HKs) | 1,1-Dichloro-2-propanone | DCP |
| 1,1,1-Trichloro-2-propanone | TCP | |
| Halonitromethanes (HNMs) | Trichloronitromethane (chloropicrin) | TCNM |
| Haloacetic acids (HAAs) | Monochloroacetic acid | MCAA |
| Monobromoacetic acid | MBAA | |
| Dichloroacetic acid | DCAA | |
| Dibromoacetic acid | DBAA | |
| Trichloroacetic acid | TCAA | |
| Bromochloroacetic acid | BCAA | |
| Bromodichloroacetic acid | BDCAA | |
| Chlorodibromoacetic acid | CDBAA | |
| Tribromoacetic acid | TBAA |
3
Comprehensive Analytical Standards for LC-MS
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LC-MS grade methanol (MeOH) and water (H2O; LC-MS grade) were obtained from VWR, Darmstadt, Germany. Acetonitrile (LC-MS grade) was purchased from Riedel-de-Haen (Honeywell, Seelze, Germany). 6-Amino-1,3-dimethyl-5-(formylamino)uracil, chlortoluron, famotidine, vidarabine, etilefrine, and 2,4-diamino-6-(hydroxymethyl)pteridine hydrochloride, and apigenin, monuron, chloridazon, carbetamide, metobromuron, sotalol, metconazol, vitexin, flavone (2-phenylchromone), DL-Ala-DL-Ala, nicotinic acid, nicotinamide, galangin, and acacetin were obtained from Merk, Darmstadt, Germany. Chlorbromuron was obtained from Dr. Ehrenstorfer, Augsburg, Germany. apigenin-6-C-arabinoside-8-C-glucoside, apigenin-6,8-di-C-glucoside, apiin, orientin, kaempferol, peonidin, norwogonin, luteolin-3,7-O-di-glucoside, 6-methoxy flavone, 4-methoxy cinnamic acid, naringenin-7-O-glucoside, isovitexin, tricin, quercetin-3-O-glucoside, saponarin, myricetin, 5-hydroxy-6-methoxy flavone, chrysoeriol, isovitexin, robinetin, and umbelliferone were kindly provided by Center of Life and Food Science Weihenstephan, Biotechnology of Natural Products, Technical University of Munich, Germany.
4
Extraction and Quantification of Benzophenone Compounds
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The chemicals were of analytical grade and had certificates of analysis. PBZ was obtained from Alfa Aesar (Lancashire, UK); BP-1 and BP-3 were acquired from AccuStandard (New Haven, CT, USA); BP, 2-OHBP, 4-OHBP, M2BB, and 4-MBP were obtained from Sigma-Aldrich (St. Louis, MO, USA), and BP-2 and BP-8 were supplied by Tokyo Chemical Industry (Tokyo, Japan). SIL-IS, BP-d5, and BP-3-d5 were purchased from Sigma-Aldrich (Burlington, MA, USA); BP-1-d5, 4-MBP-d3, BP-8-d3, diOHBP-13C6, and 4-OHBP-d4 were obtained from Toronto Research (North York, Toronto, Canada). All standards and SIL-IS had purities of >97%. Anhydrous magnesium sulfate (≥99%, MgSO4), LC-grade ACN, formic acid (≥88%), acetic acid (≥99.7%), and LC–MS-grade methanol (MeOH) were purchased from J.T. Baker (Phillipsburg, NJ, USA). Sodium chloride (>99%, NaCl) was obtained from PanReac (Castellar del Vallès, Barcelona, Spain). The bulk sorbent, Sepra C18-E and PSA, and Strata C18-T SPE cartridges (1 mL, 100 mg) were obtained from Phenomenex (Torrance, CA, USA) and Sigma-Aldrich. FaPEx-cer was obtained from Silicycle (Quebec, QC, Canada). Oasis PRiME HLB cartridges (1 mL, 30 mg) were obtained from Waters (Milford, MA, USA). The Milli-Q water of the study was produced by a Sartorius Ultrapure water system to reach a resistivity of 18.2 MΩ cm (Savska, Zaprešić, Croatia).
5
Analytical Characterization of Bioactive Compounds
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Analytical grade chemicals and reagents were used in this analysis including Quercetin (purity ≥ 95%), trans-resveratrol (purity ≥ 98%), fenofibric acid (FFA, purity ≥ 98%), and β-glucuronidase from Helix pomatia (Type H-1) (partially purified) purchased from Sigma-Aldrich (St. Louis, MO, USA). LCMS grade methanol (MeOH) and acetonitrile (ACN) purchased from JT Baker (Radnor, PA, USA) were used. Ethyl acetate from Merck Life Sciences. Pvt. Ltd. (Mumbai, India), and formic acid and ammonium acetate from Sisco Research Laboratories (SRL) Pvt. Ltd. (Mumbai, India).
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