Propagation (TGYE) medium was obtained by dissolving tryptone (0.5%), dextrose (0.1%), and Yeast extract type-1 (0.25%) in distilled water. The pH of the medium was adjusted to 7.0 followed by sterilization at 121 °C for 40 min. Store it at room temperature. The chromogenic substrate was prepared in 1 mL organic solvent with addition of 3.5 mg indoxyl acetate (chromogenic substrate) (Sigma Aldrich, U.S.A; Hi-Media, Mumbai, India). Pesticide (Supleco, Sigma-Aldrich, U.S.A.) solution prepared by diluting standard stock solution in acetonitrile (Fisher Scientific, HPLC Grade, U.K.) and stored at 4 °C. Potassium Phosphate Buffer (Hi-Media, India) (pH 6.8) was prepared by dissolving dipotassium hydrogen phosphate (K2HPO4) (0.174 g) and potassium dihydrogen phosphate (KH2PO4) (0.136 g) in 100.0 mL of distilled water and stored at room temperature.
Potassium phosphate buffer
Manufactured by HiMedia
Sourced in India
Potassium Phosphate Buffer is a laboratory chemical used to maintain a specific pH level in scientific experiments and processes. It is a common buffer solution composed of potassium salts of phosphoric acid. The buffer can be used to maintain a stable pH environment for various biochemical and analytical applications.
Automatically generated - may contain errors
3 protocols using potassium phosphate buffer
1
Colorimetric Pesticide Detection Protocol
2
Nitrocefin-Based β-Lactam Antibiotic Detection in Milk
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The propagation medium was prepared by following the protocol in our recent work24 (link). The stock solution of the chromogenic substrate nitrocefin was prepared in 500 µL organic solvent with the addition of 5 mg nitrocefin (Sigma Aldrich, U.S.A; HiMedia, Mumbai, India). ß-lactam antibiotics (HiMedia) solution was prepared by diluting standard stock solution in milk and stored at − 20 °C. Potassium phosphate buffer (Hi-Media, India) (pH 6.8) was prepared by dissolving di-potassium hydrogen phosphate (K2HPO4) (0.174 g) and potassium di-hydrogen phosphate (KH2PO4) (0.136 g) in 100.0 mL of distilled water and stored at room temperature. Raw milk samples were collected from the dairy farms near by Karnal and pasteurized milk samples were collected from the local market of Karnal, stored under refrigerated conditions, without further treatment before test.
3
Preparation of Propagation and Chromogenic Substrates for Pesticide Analysis
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Propagation (TGYE) medium was obtained by dissolving tryptone (0.5%), dextrose (0.1%), and Yeast extract type-1 (0.25%) in distilled water. The pH of the medium was adjusted to 7.0 followed by sterilization at 121 o C for 40 min. Store it at room temperature. The chromogenic substrate was prepared in 1 mL organic solvent with addition of 3.5 mg indoxyl acetate (chromogenic substrate) (Sigma Aldrich, U.S.A; Hi-Media, Mumbai, India). Pesticide (Supleco, Sigma-Aldrich, U.S.A.) solution prepared by diluting standard stock solution in acetonitrile (Fisher Scienti c, HPLC Grade, U.K.) and stored at 4°C. Potassium Phosphate Buffer (Hi-Media, India) (pH 6.8) was prepared by dissolving dipotassium hydrogen phosphate (K 2 HPO 4 ) (0.174g) and potassium dihydrogen phosphate (KH 2 PO 4 ) (0.136g) in 100.0 mL of distilled water and stored at room temperature.
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