DEAE Sepharose fast flow, Phenyl Sepharose CL-4B and Sephadex G-75 were purchased from GE Healthcare (Shanghai, China). Tris and sodium dihydrogen phosphate, dimethylsulfoxide (DMSO) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma Chemical Co. (St Louis, MO, United States). NO Griess reagent kit was obtained from Beyotime Corp (Shanghai, China). FITC anti-mouse CD86, FITC-dextran, and PE anti-mouse MHC II were obtained from Biolegend Corp (Shenzhen, Guangdong, China). DMEM medium, RPMI-1640 medium and fetal bovine serum (FBS) were purchased from Gibco Invitrogen Corp (San Diego, CA, USA). Murine TNF-α and IL-6 ELISA test kits were obtained from Excell Corp (Shanghai, China). ECL Western blotting detection kit was obtained from Tanon Crop (Shanghai, China). Anti-TLR2, anti-TLR4, anti-p-STAT3, anti-STAT3, anti-MAPKs, anti-p-MAPKs, anti-Akt, and anti-p-Akt primary antibodies were provided by Cell Signaling Technology (Beverly, MA, USA). All other reagents used in the experiments were of analytical grade.
RAW264.7 cell line and YAC-1 cell line were obtained from Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and were maintained in DMEM supplemented with 100 IU mL−1 penicillin, 100 μg/mL streptomycin and 10% FBS at 37 °C under humidified air with 5% CO2.
RAW264.7 cell line and YAC-1 cell line were obtained from Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and were maintained in DMEM supplemented with 100 IU mL−1 penicillin, 100 μg/mL streptomycin and 10% FBS at 37 °C under humidified air with 5% CO2.