Spectral-domain optical coherence tomography (SD-OCT) analysis was performed using a Spectralis SD-OCT (Heidelberg Engineering, Vista, CA, USA) imaging system coupled with a 25D lens for mouse ocular imaging (Heidelberg Engineering). Mice were anesthetized with a combination of ketamine (0.03 mg/g; IP) and xylazine (0.005 mg/g; IP) and placed on a heating pad to maintain body temperature. Pupils were dilated using a 1% tropicamide solution. The cornea was moisturized with a saline solution. Volume scans (49-line dense array, 15 A-scans per B-scan, 20° scan angle, 20° × 25° scan area) positioned directly over the optic nerve head were performed to quantify the retinal ganglion cell complex (RGCC) thickness (retinal nerve fiber layer + ganglion cell layer + inner plexiform layer), the outer nuclear layer thickness, and the total retinal thickness from the inner limiting membrane to the external limiting membrane. One single B-scan was analyzed by an individual masked to the treatment of the mouse in the superior retina, approximately 150 μm from the peripapillary region. All scans were analyzed by excluding blood vessels from the RGCC thickness calculation.
25d lens for mouse ocular imaging
Manufactured by Heidelberg Engineering
Sourced in United States
The 25D lens for mouse ocular imaging is an optical component designed for microscopy applications. It is intended to be used in conjunction with other equipment for the visualization and analysis of mouse eyes. The lens provides a specific level of magnification and focal length to facilitate the imaging and examination of small-scale ocular structures.
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4 protocols using 25d lens for mouse ocular imaging
1
Quantifying Retinal Morphology in Mice via SD-OCT
2
Retinal Ganglion Cell Complex Changes After Blast TBI
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Spectral-domain optical coherence tomography (SD-OCT) analysis was performed 2 months following blast TBI or sham procedure using a Spectralis SD-OCT (Heidelberg Engineering, Vista, CA, USA) imaging system coupled with a 25 D lens for mouse ocular imaging (Heidelberg Engineering). Anesthetized mice were placed on a temperature-controlled platform and pupils were dilated using a 1% tropicamide solution. The cornea was moisturized with a saline solution applied every 20 to 30 seconds during the procedure. Volume scans (49-line dense array) positioned directly over the optic nerve head were performed to quantify the RGC complex (inner plexiform layer + ganglion cell layer + retinal nerve fiber layer) thickness. Scans were analyzed by an individual masked to the treatment of the mouse in the superior and inferior central retina, approximately 150 μm from the peripapillary region. All scans were analyzed by excluding blood vessels from the thickness calculation. Baseline measurements were obtained 1 week prior to blast exposure, with follow-up analysis occurring 2 months post blast exposure.
3
Quantifying Retinal Ganglion Cell Changes in Diabetic Mice
4
Mouse Retinal Thickness Quantification
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A Spectralis SD-OCT (Heidelberg Engineering, Vista, CA) imaging system coupled with a 25D lens for mouse ocular imaging (Heidelberg Engineering, Vista, CA) was used. Mice anesthetized with a ketamine (55–60 mg/kg, IP) and xylazine (10–10.5 mg/kg, IP) cocktail were kept on a heating pad to maintain body temperature. Pupils were dilated with 1% tropicamide ophthalmic solution. The cornea was moisturized with balanced saline before and during recording, and after with 0.3% hypromellose. Volume scans with a pattern size of 20ox25o and 61B lines were recorded to quantify the thickness of the GCC (layers containing RGC bodies + axons + dendrites). Scans were analyzed by an individual blinded to the treatment of the mouse, in the superior retina (see Supplementary Fig. S3 online), and blood vessels were excluded from the calculation of GCC thickness.
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