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2 protocols using dipotassium hydrogen phosphate

1

Analytical Reagents and Standards

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Water, methanol, and acetonitrile were purchased from Fisher Scientific (Pittsburgh, PA, USA). Dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, diethylene glycol, and sodium carbonate were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Trichloroacetic acid was purchased from Merck Millipore Co. (Darmstadt, Germany). Methoxyamine hydrochloride, pyridine, N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA), potassium persulfate, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), Folin-Ciocalteu’s phenol, soluble starch, potassium sodium tartrate tetrahydrate, 3,5-dinitrosalicylic acid, sodium hydroxide, acetic acid, sodium acetate, and the standards 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), gallic acid, naringin, maltose, tyrosine, and p-nitro-phenol were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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2

Urs-based Electrochemical Biosensor Development

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Urs (EC 3.5.1.5, from jack beans, 15–50 U/mg; Sigma-Aldrich Co., St Louis, MO, USA) was dissolved in phosphate-buffered saline (PBS) (pH 7.4) for immobilization onto the electrode matrix. Graphite powder and aniline was purchased from Sigma-Aldrich Co. N-hydroxysuccinimide (NHS), and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) were procured from Sigma-Aldrich Co. Dipotassium hydrogen phosphate and potassium dihydrogen phosphate were purchased from JUNSEI (Kyoto, Japan). All the other reagents were of analytical grade.
Indium tin oxide (ITO) glass (<20 Ohm/sqr) was used as a base electrode. The ITO glass was cut into a rectangle of 5 mm width and 20 mm length. The working area of the ITO glass for the electrochemical tests was controlled to 0.25 cm2 using a masking tape.
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