Water, acetonitrile, and trifluoroacetic acid were purchased from Fisher Scientific (Pittsburgh, PA), formic acid from EMD chemicals (Gibbstown, NJ), and modified trypsin and ProteaseMAX from Promega (Madison, WI). A Peptide CapTrap (0.5-mm ID × 2-mm that according to the manufacture produces C8-like retention and referred to hereafter as a C8 trap) and the 0.2-mm ID × 50-mm C18 AQ (3 μm) column were purchased from Bruker-Michrom (Auburn, CA). The PGC column was a Hypercarb® 0.18-mm ID × 100 mm (5 μm) from ThermoFisher (West Palm Beach, FL). LC-MS instrumentation included a Shimadzu Prominence HPLC (Columbia, MD), a ThermoFisher Surveyor HPLC pump and a ThermoFisher LTQ MS.
Surveyor hplc pump
Manufactured by Thermo Fisher Scientific
The Surveyor HPLC pump is a high-performance liquid chromatography pump designed for precision and reliability in analytical applications. It delivers stable and consistent flow rates to ensure accurate and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
Modified trypsin, by Promega (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Prominence hplc, by Thermo Fisher Scientific (1 mentions)
Proteasemax, by Promega (1 mentions)
Ltq velos orbitrap elite, by Thermo Fisher Scientific (1 mentions)
2 protocols using surveyor hplc pump
1
Reversed-Phase C18 and PGC LC-MS
2
Protein Identification via Mass Spectrometry
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Protein bands of interest were cut from Coomassie R250-stained polyacrylamide gels. In gel digestion of proteins was performed according to [25 (link)] with minor modifications. Mass spectrometry analyses were performed on a Thermo Scientific™ LTQ-Orbitrap Velos Elite mass spectrometer combined with a Surveyor HPLC pump (Thermo Fisher Scientific). Peaks Studio (version 7.5; BSI, Waterloo, ON, Canada) was used to analyze the mass spectra against the staphylococcal database. For specific identification, proteins were accepted if they could be established at a Peaks protein probability score (−10 lgP) better than 20 with a minimum of two unique peptides per protein. For the full description of used mass spectrometry methods please see the Supplementary section.
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