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Jem 2100uhr microscope

Manufactured by JEOL
Sourced in Japan

The JEM-2100UHR is a high-resolution transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-quality imaging and analysis capabilities for a wide range of materials and applications. The JEM-2100UHR features a LaB6 electron source, advanced optics, and a high-resolution CCD camera to deliver exceptional image resolution and contrast. It is capable of operating in a variety of modes, including bright-field, dark-field, and high-resolution imaging, as well as selected area electron diffraction (SAED) and energy-dispersive X-ray spectroscopy (EDS) analysis.

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4 protocols using jem 2100uhr microscope

1

Characterization of MnPc@P Nanostructures

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Transmission electron microscopy (TEM) images were acquired on a JEM-2100UHR microscope (JEOL, Japan) to characterize the morphology. X-ray photoelectron spectroscopy (XPS, ESCALAB 250Xi, Japan) was applied to analyze the chemical compositions of MnPc@P. UV-vis-NIR spectrum was investigated by using an Infinite M200 PRO spectrophotometer. The hydrodynamic particle size and zeta potential of MnPc@P was monitored by dynamic light scattering (DLS, 15 mW laser, 676 nm incident beam; Brookhaven Instruments Corporation).
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2

Microscopic Characterization of Composite Biomaterials

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The distribution of vancomycin grains and additives in the PMMA matrix as well as the MFC shape and length were investigated with the help of scanning electron microscopy (SEM). The samples retrieved after the elution study and microhardness test were crashed in the mortar and covered by a 10–15 nm gold layer via magnetron sputtering using Quorum Technologies Q150R Plus sputter coater. The JEOL JIB 4501 multibeam system (Akishima, Tokyo) was used, and the SEM micrographs were acquired in BSE mode at an accelerating voltage of 10 kV.
To study the form and structure of the employed HNT, NCC and NFC transmission electron microscopy (TEM) was employed. The corresponding micrographs were acquired using the JEOL JEM 2100 UHR microscope in TEM brightfield mode at an accelerating voltage of 200 kV. To enhance the contrast of cellulose fibrils with low electron-scattering power, the negative staining (using a 2% solution of phosphotungstic acid, pH = 7.0) was applied.
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3

Multimodal Characterization of Nanoparticles

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To characterize the microstructure and morphology of nanoparticles, transmission electron microscopy (TEM) was performed on a JEM-2100UHR microscope (JEOL, Japan). X-ray photoelectron spectroscopy (XPS, ESCALAB 250Xi, Japan), X-ray diffraction (XRD, Bruker D8, Germany), and Fourier transform infrared spectrophotometer spectrum (FTIR, Nicolet6700-Contiuμm, Thermo, USA) were carried out to analyze the chemical compositions and crystal structures of HES@CuP. UV-vis-NIR spectrum of HES@CuP and HES@CuP-D was investigated by using an Infinite M200 PRO spectrophotometer. The zeta potential and corresponding hydrodynamic particle size of HES@CuP and HES@CuP-D were detected through dynamic light scattering (DLS, 90Plus PALS, Brookhaven, UK). The scheme illustration was drawn by Figdraw.
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4

Comprehensive Characterization of CaS Nanoparticles

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Transmission electron microscopy (TEM) images were acquired on a JEM-2100UHR microscope (JEOL, JEM-2100UHR, Tokyo, Japan). X-ray photoelectron spectroscopy (XPS, Thermo Fisher Scientific Inc., ESCALAB 250Xi, Asheville, NC, USA) and energy dispersive spectrometer (EDS) were applied to analyze the chemical constitutions of CaS, CaS@PP and I-CaS@PP. X-ray diffraction (XRD, Bruker, Bruker D8, Karlsruhe, Germany) was performed to analyze the crystal structure of CaS and I-CaS@PP. The hydrodynamic particle sizes and zeta potentials of NPs were monitored by dynamic light scattering (DLS, Brookhaven Instruments Corporation, NanoBrook 90 Plus PALS, New York, NY, USA). The loading amount of ICG in I-CaS@PP was determined based on the UV–Vis absorbance peak at 780 nm, and it was calculated according to the followed Eq. (3):
The loading amount of CaS in I-CaS@PP was determined by detecting Ca and S amounts in I-CaS@PP using ICP-MS (Thermo Fisher Scientific Inc., Thermo ICAP PRO, NC, USA).
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