Purified recombinant proteins GST‐SRL10, His‐CATB and total proteins from 20‐d‐old seedlings of WYJ7 were used for cell‐free degradation assay, and was carried out as previously described (Bello et al., 2019 (link); Lv et al., 2014 (link)). The dilution for the mouse anti‐GST antibody (TransGen Biotech) and the mouse anti‐His antibody (TransGen Biotech) was 1:5000. Reactions were terminated at indicated time points, and the protein abundance was visualized via immune detection against anti‐His and anti‐GST. Total proteins from NIL‐SRL10Hap1 and NIL‐SRL10Hap3 were used for thermal stability analysis of protein and incubated under 28 °C and 42 °C for 0, 30, 60 and 90 min respectively. The dilution for the rabbit anti‐SRL10 antibody was 1:10000.
Mouse anti gst antibody
Manufactured by Transgene
Sourced in China
The Mouse anti-GST antibody is a laboratory reagent used for the detection and purification of proteins fused with the Glutathione S-Transferase (GST) tag. This antibody specifically binds to the GST tag, allowing for the identification and isolation of GST-tagged proteins in various applications.
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Lab products found in correlation
2 protocols using mouse anti gst antibody
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Thermal Stability of SRL10 Protein
2
Antibodies and Concentration for Western Blot
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The following antibodies and working concentrations were used: Mouse anti-tubulin antibody (Cat#T6074, Sigma-Aldrich, St Louis, MO, USA, 1:3000 for Western blot), Rabbit anti-Bip antibody (Cat#AB310, Beyotime, Shanghai, China, 1:1000 for Western blot), Rabbit anti-Xbp1s antibody (Cat#AF8366, Beyotime, Shanghai, China, 1:1000 for Western blot), Rabbit anti-Chop antibody (Cat#T6074, Affinity Biosciences, Cincinnati, OH, USA, 1:1000 for Western blot), Mouse anti-SREBP antibody (produced in-house, 1:1000 for Western blot), Rabbit anti-hemocyanin antibody (produced in-house, 1:3000 for Western blot), Rabbit anti-V5 antibody (Cat#D191104, Sangon Biotech, Shanghai, China, 1:1000 for Western blot), Mouse anti-FLAG antibody (Cat#D110005, Sangon Biotech, Shanghai, China, 1:1000 for Western blot), Mouse anti-GST antibody (Cat#HT601-01, 1:1000, TransGen Biotech, Beijing, China, 1:1000 for Western blot), Horseradish peroxidase (HRP)-linked goat anti-rabbit (Cat#31460, Thermo Fisher Scientific, Waltham, MA, USA, 1:3000, for Western blot), and HRP-linked goat anti-mouse (Cat#A32723, Thermo Fisher Scientific, Waltham, MA, USA, 1:3000 for Western blot).
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