Adiponectin elisa kit

Manufactured by R&D Systems

Sourced in United States

The Adiponectin ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of Adiponectin in human serum, plasma, and cell culture supernatants.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Leptin elisa kit, by R&D Systems (1 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Rosiglitazone, by Cayman Chemical (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Nile red, by Thermo Fisher Scientific (1 mentions) Barnstead nanopure system, by Thermo Fisher Scientific (1 mentions) Methylisobutylxanthine ibmx, by Merck Group (1 mentions) Polyvinyl alcohol pva mw 13 000 23 000, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem with 4.5 g l glucose and l glutamine, by Corning (1 mentions) Dichloromethane dcm, by Merck Group (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Mk 5000rq, by Muromachi Kikai (1 mentions) Total cholesterol kit, by Fujifilm (1 mentions) Free fatty acid determination kit, by Fujifilm (1 mentions) Triglyceride determination kit, by Fujifilm (1 mentions) Leptin, by Thermo Fisher Scientific (1 mentions) Anti pparγ, by Santa Cruz Biotechnology (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Anti c ebpα, by Santa Cruz Biotechnology (1 mentions)

6 protocols using adiponectin elisa kit

Quantifying Serum Leptin and Adiponectin

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Serum levels of leptin and adiponectin were analysed using leptin ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA) and adiponectin ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA), according to the manufacturer’s protocols.

Lee J.H., Moon J.M., Kim Y.H., Lee B., Choi S.Y., Song B.J., Kim D.K, & Lee Y.M. (2019). Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice. Nutrients, 11(2), 269.

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PLGA Microparticles for Adipogenic Differentiation

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75:25 poly (D,L-lactide-co-glycolide) (PLG) with a lauryl ester end group and an inherent viscosity of 0.79 dL/g was purchased from Evonik (Birmingham, AL). Rosiglitazone was purchased from Cayman Chemical (Ann Arbor, MI). Dichloromethane (DCM) and polyvinyl alcohol (PVA, Mw 13,000–23,000, 87–89% hydrolyzed) were purchased from Sigma (St. Louis, MO). Ethyl acetate was purchased from Macron Fine Chemicals (Center Valley, PA). 200 proof ethanol was purchased from Decon Laboratories (King of Prussia, PA). Dimethyl sulfoxide (DMSO) was purchased from Fisher (Hampton, NH). Ultrapure water was obtained from a Thermo Scientific Barnstead Nanopure system. Dulbecco’s Modified Eagle’s Medium (DMEM) with 4.5 g/L glucose and L-glutamine was purchased from Corning Cellgro (Corning, NY). Trypsin with 0.25% EDTA, Fetal Bovine Serum (FBS) and Pen/Strep were purchased from Fisher (Hampton, NH). Super Calf Serum was purchased from GemCell (Sacramento, CA). Insulin (bovine), dexamethasone, and methylisobutylxanthine (IBMX) were purchased from Sigma (St. Louis, MO). 10% Neutral Buffered Formalin was purchased from Sigma (St. Louis, MO). Nile Red was purchased from Molecular Probes (Eugene, OR). Adiponectin ELISA kit was purchased from R&D systems (Minneapolis, MN).

Spetz M.R., Isely C, & Gower R.M. (2021). Effect of fabrication parameters on morphology and drug loading of polymer particles for rosiglitazone delivery. Journal of drug delivery science and technology, 65(1-2), 10.1016/j.jddst.2021.102672.

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Metabolic Characterization of Mouse Glucose and Insulin Homeostasis

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Body weight and food intake were measured every week. Whole-body O₂ consumption and CO₂ production were monitored using an O₂/CO₂ metabolism measuring system for small animals (MK-5000RQ, Muromachi Kikai). In GTT experiments, 17-week-old mice were injected intraperitoneally with glucose (1 g per kg body weight) after a 4-h fast. In ITT experiments, 19-week-old mice were injected intraperitoneally with insulin (0.75 U per kg body weight) after a 4-h fast. Blood glucose levels were measured at the indicated time points using Glutest Neo alpha (Sanwa Kagaku). Unless otherwise specified, blood samples were collected under 4-h-fasted conditions. The following kits were used to determine metabolic parameters: Triglyceride Determination Kit (Wako), Total Cholesterol Kit (Wako), Free Fatty Acid Determination Kit (Wako), Insulin ELISA kit (Morinaga), Leptin ELISA kit (Morinaga) and Adiponectin ELISA kit (R&D Systems).

Yamamuro T., Kawabata T., Fukuhara A., Saita S., Nakamura S., Takeshita H., Fujiwara M., Enokidani Y., Yoshida G., Tabata K., Hamasaki M., Kuma A., Yamamoto K., Shimomura I, & Yoshimori T. (2020). Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nature Communications, 11, 4150.

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Inflammatory Signaling Modulation Protocol

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Benzamidine, bovine serum albumin (BSA), EDTA, HEPES, leupeptin, phenylmethylsulfonyl fluoride (PMSF), soybean trypsin inhibitor, and trypsin were obtained from Sigma-Aldrich (St. Louis, MO, USA). TLR4 antagonist TAK-242 (TAK) was obtained from the Cayman Chemical Company (Ann Arbor, MI, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were acquired from GIBCO (Carlsbad, CA, USA). Ready-To-Glow pNF-κB-secreted luciferase reporter system was obtained from Clontech (Mountain View, CA, USA). Antibodies anti-C/EBPα and anti-PPARγ were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody anti-actin was purchased from Abcam (Cambridge, UK). Enzyme-linked immunosorbent assay (ELISA) kits (IL-6, IL-1β, Leptin, and TNFα) were purchased from Peprotech (Rocky Hill, NJ, USA). The adiponectin ELISA kit was purchased from R&D systems (Minneapolis, MN, USA). CM-H₂DCFDA (DCF) was from Life Technologies (Carlsbad, CA, USA). The enhanced chemiluminescence (ECL) and BCA protein assay kit were purchased from Pierce Biotechnology (Waltham, MA, USA).

Renovato-Martins M., Moreira-Nunes C., Atella G.C., Barja-Fidalgo C, & de Moraes J.A. (2020). Obese Adipose Tissue Secretion Induces Inflammation in Preadipocytes: Role of Toll-Like Receptor-4. Nutrients, 12(9), 2828.

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Serum Biomarker Quantification in Mice

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Serum insulin, leptin, and adiponectin levels were measured with ELISAs for mouse insulin (ALPCO Diagnostics, Salem, NH, USA), adiponectin ELISA Kit (R&D Systems, Oxon, UK), and leptin (AdipoGen, Seoul, Korea) according to the manufacturer's instructions.

Cai W., Yu L., Zhang Y., Feng L., Kong S., Tan H., Xu H, & Huang C. (2016). Extracts of Coreopsis tinctoria Nutt. Flower Exhibit Antidiabetic Effects via the Inhibition of α-Glucosidase Activity. Journal of Diabetes Research, 2016, 2340276.

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Quantifying Growth Factors in Bioreactor

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To determine the amount of growth factors in bioreactor supernatant before implantation, culture medium after the 1 week culture period was analyzed using VEGF ELISA kit (#DY293B, R&D Systems), IGF-1 ELISA kit (#DY291, R&D Systems), Adiponectin ELISA kit (#DY1065, R&D Systems), and Thrombospondin ELISA kit (#ab193716, Abcam). The concentration of growth factors was normalized to the total amount of protein measured by the BCA quantification kit.

Guerrero J., Dasen B., Frismantiene A., Pigeot S., Ismail T., Schaefer D.J., Philippova M., Resink T.J., Martin I, & Scherberich A. (2022). T-cadherin Expressing Cells in the Stromal Vascular Fraction of Human Adipose Tissue: Role in Osteogenesis and Angiogenesis. Stem Cells Translational Medicine, 11(2), 213-229.

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