Proliferation of HUVECs was evaluated by a CCK-8 Kit (Liankebio, China) in conformity to the instruction of the manufacturer. In brief, HUVECs were digested with trypsin, and 5 × 103 cells were plated into each well of a 96-well plate (BD Falcon). After being cultured in a complete medium for 24 hours, the cells were then cultured in hAEC-CM for another 72 hours. Complete medium was treated as a positive control medium, and serum-free high-glucose DMEM was used as a negative control medium.
Cck8 kit
Manufactured by Liankebio
Sourced in China
The CCK8 kit is a colorimetric assay for determining cell viability and cytotoxicity. It uses a water-soluble tetrazolium salt to measure the number of viable cells.
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Lab products found in correlation
6 protocols using cck8 kit
1
Proliferation of HUVECs in hAEC-CM
2
Cell Proliferation Monitoring by CCK-8 Assay
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Cell proliferation was monitored with the cell counting kit-8 (CCK-8) kit (Lianke Bio, China). Cells were harvested after treatment at the exponential proliferation phase and plated onto 96-well plates, followed by 1-, 2-, and 3-days incubation at 37 ℃. CCK-8 solution was added to each well and allowed to incubate for 10 min. The absorbance of each well was measured at 450 nm.
3
Evaluating Ovarian Cancer Cell Proliferation
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CCK8 kit (Lianke Bio, China) and EDU assay kit (RIBOBIO, China) were used to determine cell proliferation of ovarian cancer cell. CCK8 assay: 105 cells were plated in 96-well plate after transfection with siRNA or NC. The cells were transferred into an incubator for 24, 48, and 72 h. Finally, CCK8 working reagent was added to the wells and incubated for 2 h. Culture solution was read at 450 nm absorbance.
4
Cell Viability Assay Protocol
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Twenty‐four hours after transfection, cells were cultured in 96‐well plates (1 × 104 cells/well). Desired drugs and compounds were added to the wells separately immediately after cells were seeded and cultured with the cells for 24 hr. Cell viability was measured by fluorescence chemistry using a CCK8 kit (Lianke Bio, Beijing, China) with a spectrophotometer (Multiskan MK3; Thermo Fisher Scientific, Rockford, IL) at an optical density (OD) of 450 nm.
Each experimental condition was repeated three times with up to five multiple wells each time. Data are presented as the mean ± standard error, and statistical significance was calculated by Student’s t test in Excel. P < 0.05 was considered significantly different.
Each experimental condition was repeated three times with up to five multiple wells each time. Data are presented as the mean ± standard error, and statistical significance was calculated by Student’s t test in Excel. P < 0.05 was considered significantly different.
5
Cell Viability Assay Protocol
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For the viability assays, cells were seeded in 96-well plates at 1×104 cells per well. For short-term treatments, selected drugs, compounds, and extracts were added to the wells immediately afterwards and cultured with the cells for 24 h. For long-term treatments, fresh medium with reagents were added every 24 h. The cell viability was measured by fluorescence chemistry with a spectrophotometer (Thermo, Multiscan MK3, USA) using the CCK8 kit (Lianke Bio, China) at OD 405 nm. Cell viability was calculated from the OD values in comparison with the blank. The inhibition rate was calculated by the following formula: inhibition rate (%)=100*(ODctrl–ODtreated)/ODctrl. Each treatment condition was repeated 4 times with at least 3 repeated wells each time.
6
Cell Viability Assessment via CCK8 Assay
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Cell viability was detected by the CCK8 kit (Lianke Bio, China). Briefly, the cells were digested after 24 h transfection to prepare cell suspension. Then, cell suspension was plated in 96-well plates, and the number of cells in each well was about 1 × 103. The 96-well plates were incubated under standard conditions, and cell viability was examined every 24 h. A total of 10 µL CCK8 was added into each well at the indicated time points and incubated for 1.5 h at 37°C. Finally, the optical density (OD) value at 450 nm was detected, and the proliferation curve was drawn.
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