Immunohistochemistry was performed on paraffin tissue sections (4μm) using a microwave-based antigen retrieval technique37 . The antibody used in this study including sheep polyclonal antibody to BrdU (Abcam, Cambridge, UK), rabbit polyclonal antibody to PCNA or p-Smad2/3 (Santa Cruz).
Sheep polyclonal antibody to brdu
Manufactured by Abcam
Sourced in United Kingdom
Sheep polyclonal antibody to BrdU is a laboratory reagent used for the detection of bromodeoxyuridine (BrdU), a thymidine analog incorporated into DNA during cell proliferation. This antibody is produced in sheep and recognizes BrdU in a non-specific manner.
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2 protocols using sheep polyclonal antibody to brdu
1
Immunohistochemistry of Cell Proliferation
2
Histological Evaluation of Kidney Injury
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The kidney samples were fixed in 4% paraformaldehyde, stained with periodic acid-Schiff staining, and observed by light microscopy. The percentage of tubular necrosis was scored from at least 10 randomly chosen fields by microscopy (×200) as previously reported [23 (link)].
IHC was performed on 5-mm kidney sections by incubating with sheep polyclonal antibody to BrdU (Abcam, Cambridge, UK), rabbit polyclonal antibody to proliferating cell nuclear antigen (PCNA), Ki67 or p-Smad2/3 (Abcam), followed by incubation with the relevant peroxidase-conjugated secondary antibodies. The quantification of positive tubular regions was performed by examining at least 10 fields (×200) for each mouse.
IHC was performed on 5-mm kidney sections by incubating with sheep polyclonal antibody to BrdU (Abcam, Cambridge, UK), rabbit polyclonal antibody to proliferating cell nuclear antigen (PCNA), Ki67 or p-Smad2/3 (Abcam), followed by incubation with the relevant peroxidase-conjugated secondary antibodies. The quantification of positive tubular regions was performed by examining at least 10 fields (×200) for each mouse.
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