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Laser capture palm system

Manufactured by Zeiss
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The Laser Capture PALM system is a versatile tool for isolating specific cells or subcellular structures from complex samples. The system utilizes a laser-based microdissection technique to precisely extract the desired target material for further analysis.

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Lab products found in correlation

Membraneslide 1.0 pen slides, by Zeiss (2 mentions) Picopure dna extraction kit, by Arcturus (2 mentions) Qiaamp dna micro kit, by Qiagen (1 mentions) Palm membrane slides, by Zeiss (1 mentions) Lightcycler 480 real time pcr system, by Roche (1 mentions) Mirneasy kit, by Qiagen (1 mentions)

4 protocols using laser capture palm system

1

Laser Capture Microdissection for DNA Extraction

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MembraneSlide 1.0 PEN slides (Zeiss) were exposed to UV light for 30 min before mounting 8 μm sections. These slides were baked at 37°C for 30 min, then dewaxed for 5 min and rehydrated through graded alcohols to water for 3–5 min each. The slides were then briefly dipped in methyl green, washed in water and dried at 37°C for 1 h. Laser capture microdissection was performed with the laser capture PALM system (Zeiss). DNA was extracted using the PicoPure DNA extraction kit (Arcturus).
Davis H., Irshad S., Bansal M., Rafferty H., Boitsova T., Bardella C., Jaeger E., Lewis A., Freeman-Mills L., Giner F.C., Rodenas-Cuadrado P., Mallappa S., Clark S., Thomas H., Jeffery R., Poulsom R., Rodriguez-Justo M., Novelli M., Chetty R., Silver A., Sansom O.J., Greten F.R., Wang L.M., East J.E., Tomlinson I, & Leedham S.J. (2014). Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside of the crypt base stem cell niche. Nature medicine, 21(1), 62-70.
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2

Laser Capture Microdissection for DNA Extraction

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MembraneSlide 1.0 PEN slides (Zeiss) were exposed to UV light for 30 min before mounting 8 μm sections. These slides were baked at 37°C for 30 min, then dewaxed for 5 min and rehydrated through graded alcohols to water for 3–5 min each. The slides were then briefly dipped in methyl green, washed in water and dried at 37°C for 1 h. Laser capture microdissection was performed with the laser capture PALM system (Zeiss). DNA was extracted using the PicoPure DNA extraction kit (Arcturus).
Davis H., Irshad S., Bansal M., Rafferty H., Boitsova T., Bardella C., Jaeger E., Lewis A., Freeman-Mills L., Giner F.C., Rodenas-Cuadrado P., Mallappa S., Clark S., Thomas H., Jeffery R., Poulsom R., Rodriguez-Justo M., Novelli M., Chetty R., Silver A., Sansom O.J., Greten F.R., Wang L.M., East J.E., Tomlinson I, & Leedham S.J. (2014). Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside of the crypt base stem cell niche. Nature medicine, 21(1), 62-70.
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3

Laser-Capture Microdissection of Mammary Tissue

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Frozen sections (16 μm thick) were cut onto PALM membrane slides (Zeiss) and air‐dried at room temperature for 1 h and then subjected to enzymatic CCO staining as described above. Single cells or larger areas of interest from mammary ducts and terminal duct lobular units (TDLUs) were then microdissected on a PALM laser capture system (Zeiss) at a uniform laser power and cutting width into PALM‐specific 0.5 ml tubes. Stromal tissue was used as a control from each section. DNA was extracted using QIAamp DNA Micro kits (Qiagen, Hilden, Germany) according to the manufacturer's protocol.
Cereser B., Jansen M., Austin E., Elia G., McFarlane T., van Deurzen C.H., Sieuwerts A.M., Daidone M.G., Tadrous P.J., Wright N.A., Jones L, & McDonald S.A. (2017). Analysis of clonal expansions through the normal and premalignant human breast epithelium reveals the presence of luminal stem cells. The Journal of Pathology, 244(1), 61-70.
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4

MicroRNA profiling of mouse limbal and corneal basal cells

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Eyes from wild-type mice (Balb/c) were embedded in optimal cutting temperature (OCT) compound and stored at −80 °C until sectioning. Basal cells from limbal and corneal epithelia from 5 μm frozen sections were isolated and captured using a PALM laser capture system (Zeiss) as previously described[39 (link)]. Total cellular RNA from LCM-captured cells was isolated and purified by miRNeasy kit (Qiagen). microRNA profiling was performed using Exiqon's miRCURY LNA Universal RT miRNA PCR Rodent panel I and II by Exiqon. Briefly, 5 ul RNA was reverse transcribed in 40μl reactions using the miRCURY LNA™ Universal RT microRNA PCR, Polyadenylation and cDNA synthesis kit (Exiqon). cDNA was diluted 100 × and assayed in 10 ul PCR reactions according to the protocol for miRCURY LNA™ Universal RT microRNA PCR; each microRNA was assayed once by qPCR on the microRNA Ready-to-Use PCR, Rodent panel I and panel II. Negative controls excluding template from the reverse transcription reaction was performed and profiled like the samples. The amplification was performed in a LightCycler® 480 Real-Time PCR System (Roche) in 384 well plates. The amplification curves were analyzed using the Roche LC software, both for determination of Cp (by the 2nd derivative method) and for melting curve analysis.
Peng H., Park J.K., Katsnelson J., Kaplan N., Yang W., Getsios S, & Lavker R.M. (2015). microRNA-103/107 family regulates multiple epithelial stem cell characteristics. Stem cells (Dayton, Ohio), 33(5), 1642-1656.
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