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4 protocols using hplc grade water

1

Synthesis of Copper-Alginate Composite

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Sodium alginate, calcium chloride, benzene, copper sulfate, nitric acid, ethanol, and HPLC-grade water were purchased from Samchun Pure Chemical Co., Ltd. (Gyeonggi-do, Republic of Korea). Sodium citrate was purchased from Deajung Chemical & Metals Co., Ltd. (Gyeonggi-do, Republic of Korea). Wood powder from pitch pine (Pinus rigida) was obtained from G-Biotech (Seoul, Republic of Korea). All the other chemicals used in this study were of analytical grade and used without further purification.
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2

Antioxidant and Antimicrobial Evaluation

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Vanillyl alcohol (4-hydroxy-3-methoxybenzyl alcohol, VA), glyceryl trioctanoate (tricaprylin, TCN), glyceryl tributyrate (tributyrin, TBN), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,6-di-tert-butyl-4-methylphenol (BHT), Menhaden oil (MO), 1-butanol, and bacterial propidium iodide (PI) solution were purchased from Sigma-Aldrich Co. (USA). Acetone and toluene were purchased from Junsei Co. (Japan), while methanol was purchased from Merck Co. (Germany). methanol and HPLC grade water were purchased from Samchun Chemicals Co.(Korea), and methacrylate-divinylbenzene (MA-DVB) resin was purchased from GenoFocus (Korea). LB broth was purchased from Becton, Dickinson and Co. (USA).
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3

Profiling Ginsenoside Compounds

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HPLC-grade water (J.T. Baker, Center Valley, PA, USA) and acetonitrile (Samchun, Seoul, South Korea) were used as HPLC eluents. Milli-Q water from Millipore (Darmstadt, Germany) was also used as a solvent, except in HPLC measurements. Ethanol, used as an extraction solvent, was special grade (Samchun, Seoul, South Korea), whereas ethanol used for antioxidant activity measurements was HPLC grade (Fischer Scientific, Pittsburgh, PA, USA). HPLC-grade methanol (J.T. Baker, Center Valley, PA, USA) was used to check the antioxidant activity. Standard ginsenosides, Rg1, Re, Rf, Rb1, Rc, Rb2, Rd, and 20(S)-Rg3 were purchased from ChromaDex (Irvine, CA, USA). Rk1 and Rg5 were obtained from Ambo Institute (Daejoen, South Korea). 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid; ABTS), Folin–Ciocalteu reagent, 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox), gallic acid, and acrylamide were purchased from Sigma–Aldrich (St. Louis, MO, USA).
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4

Antioxidant Activity Evaluation Protocol

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Vanillyl alcohol (4-hydroxy-3-methoxybenzyl alcohol, VA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,6-di-tert-butyl-4-methylphenol (BHT), 2,2'-azino-bis (3-ethylbenzothiazoline-6sulphonic acid) (ABTS), Menhaden oil (MO), bacterial propidium iodide (PI) solution, and 1butanol were purchased from Sigma-Aldrich Co. (USA). Toluene, acetone, and potassium persulfate were purchased from Junsei Co. (Japan), while methanol was purchased from Merck Co. (Germany). Linseed oil, methanol and HPLC grade water were purchased from Samchun Chemicals Co. (Korea). LB broth was purchased from Becton, Dickinson and Co.
(USA). Candida antartica lipase (CalB) immobilized on acrylic resin was purchased from Sigma-Aldrich Co. (USA).
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