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Plcγ1 antibody

Manufactured by Cell Signaling Technology
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Sourced in United States

The PLCγ1 Antibody is a reagent used for the detection and analysis of the phospholipase C gamma 1 (PLCγ1) protein in various biological samples. PLCγ1 is an enzyme that plays a key role in cellular signal transduction pathways. This antibody can be utilized in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of PLCγ1 in different cell types and tissues.

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Lab products found in correlation

Phospho stat5, by Cell Signaling Technology (1 mentions) Jak1 antibody, by Cell Signaling Technology (1 mentions) β actin antibody, by Cell Signaling Technology (1 mentions) Phospho stat6, by Cell Signaling Technology (1 mentions) Pkcζ antibody, by Cell Signaling Technology (1 mentions) Phospho stat2, by Cell Signaling Technology (1 mentions) Ras antibody, by Cell Signaling Technology (1 mentions) Mmp 9 antibody, by Cell Signaling Technology (1 mentions) Phospho jak2, by Cell Signaling Technology (1 mentions) Phospho stat1, by Cell Signaling Technology (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions) Image lab software, by Bio-Rad (1 mentions) α tubulin antibody, by Merck Group (1 mentions) Erk1 2 antibody, by Cell Signaling Technology (1 mentions) 0.22 μm pvdf membranes, by GE Healthcare (1 mentions) Vegfr2 antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

PLCγ1 (37 citations) Rabbit anti-phospho-PLCγ1 (Tyr783) antibody (2 citations)

2 protocols using plcγ1 antibody

1

Protein Kinase Signaling Pathway Analysis

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The following antibodies were purchased from Cell Signaling (Danvers, MA, USA): JAK1 Antibody (#3332, 130kDa), Phospho-JAK2 (Tyr1007/1008) Antibody (#3771, 125kDa), β-Actin Antibody (#4967, 45kDa), Phospho-STAT1 (Tyr701) (58D6) Rabbit mAb (#9167, 84,91kDa), Phospho-STAT2 (Tyr690) Antibody (#4441, 113kDa), Phospho-STAT3 (Tyr705) Antibody (#9131, 79,86kDa), Phospho-STAT5 (Tyr694) Antibody (#9351, 90kDa), Phospho-STAT6 (Tyr641) Antibody (#9361, 110kDa), Ras Antibody (#3965, 21kDa), p44/42-MAPK (Erk1/2) Antibody (#9102, 42,44kDa), GSK-3β (27C10) Rabbit mAb (#9315, 46kDa), MMP-9 Antibody (#3852, 84,92kDa), Phospho-PKCα/β II (Thr638/641) Antibody (#9375, 80,82kDa); PKCα Antibody (#2056, 80kDa); Phospho-PKC (pan) (βII Ser660) Antibody (#9371, 78 a 85kDa); Phospho-PKD/PKCμ (Ser744/748) Antibody (#2054, 115kDa); PKD/PKCμ Antibody (#2052, 115kDa); PKCδ Antibody (#2058, 78kDa); Phospho-PKCδ/θ (Ser643/676) Antibody (#9376, 78kDa), PKCζ Antibody (#9372, 78kDa); PLCγ1 Antibody (#2822, 155kDa); anti-mouse, anti-rabbit and anti-goat IgGs antibodies. From Abcam (Cambridge, MA, USA): anti-PKC antibody (ab59363) was purchased. PepChip1-Kinomics™ slides were obtained from Pepscan Presto BV (Lelystad, the Netherlands).
WP1066 (#573097; C17H14BrN3O) and Tofacitinib (#PZ0017; C16H20N6O · C6H8O7) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Nascimento A.S., Peres L.L., Fari A.V., Milani R., Silva R.A., da Costa Fernandes C.J., Peppelenbosch M.P., Ferreira-Halder C.V, & Zambuzzi W.F. (2017). Phosphoproteome profiling reveals critical role of JAK-STAT signaling in maintaining chemoresistance in breast cancer. Oncotarget, 8(70), 114756-114768.
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2

VEGF-Induced Signaling Pathway Analysis

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Confluent HDMECs were stimulated by 50 ng/ml VEGF-A for indicated lengths of time and lysed in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1% NP-40, 0.1% sodium deoxycholate, 1 mM EDTA, all from Sigma-Aldrich, USA) supplemented with protease and phosphatase inhibitors (Mini Complete/PhosphoStop, Roche Diagnostics, Germany). Protein contents were determined using the BCA Protein Assay Kit (Pierce, USA). Protein extracts were separated on 7.5% stain-free precast gels (Bio-Rad, USA) and transferred to 0.22 μm PVDF membranes (GE Healthcare, USA). Blots were probed with primary antibodies, including phospho-VEGFR2 Y1175 antibody (rabbit, 1:1000), VEGFR2 antibody (rabbit, 1:1000), EphrinB2 antibody (rabbit, 1:500), phospho-PLCγ1 Y783 antibody (rabbit, 1:1000), PLCγ1 antibody (rabbit, 1:1000), phospho-Akt Thr308/473 antibody (rabbit, 1:1000), Akt1/2 antibody (rabbit, 1:1000), phospho-p44/42 MAPK Thr202/Tyr204 antibody (rabbit, 1:2000) and Erk1/2 antibody (rabbit, 1:2000; all from Cell Signaling Technology, USA), and α-tubulin antibody (mouse,1:2000, Sigma-Aldrich, USA), and subsequently with secondary anti-mouse/ -rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad, USA) and normalization to total protein.
Xiong Y., Scerbo M.J., Seelig A., Volta F., O'Brien N., Dicker A., Padula D., Lickert H., Gerdes J.M, & Berggren P.O. (2020). Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling. eLife, 9, e56914.
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