Gas chromatography-mass spectrometry analysis was performed with a Thermo Fisher TRACE GC ULTRA (Thermo Fisher Scientific, Bangalore) coupled with DSQ II Mass Spectrometer instrument (Thermo Fisher Scientific, Bangalore) using a TR 50MS column (30 m × 0.25 mm ID × 0.25 μm, film thickness); carrier gas, helium; temperature programming, 5 min delay for solvent, at 50°C temperature, hold time 5.0 min, rising at 4°C/min to 250°C and finally held isothermally for 5 min. The injector temperature was 230°C and carrier flow was constant at 1 mL/min, in split mode (1:50) with injector volume 1 μL. The ion source temperature was set at 220°C, transfer line temperature was 300°C, and the ionization of the sample components was performed in electron ionization mode at an ionization voltage of 70eV. Mass range was used from m/z 50 to 650 amu.
Thermo fisher trace gc ultra
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The Thermo Fisher TRACE GC ULTRA is a gas chromatograph designed for analytical applications. It features a high-performance, dual-stage vacuum system and is capable of handling a wide range of sample types. The TRACE GC ULTRA provides precise control of chromatographic parameters for accurate and reproducible results.
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Lab products found in correlation
2 protocols using thermo fisher trace gc ultra
1
GC-MS Analysis of Organic Compounds
2
Quantitative GC-MS Analysis of Ethylene
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Samples for gas chromatography measurements were taken from the shake flask headspace during the gas flow phase of the RAMOS measurement cycle. Gas sampling bags (0.3 L, PVDF, Chemware, Raleigh, North Carolina, USA) were attached to the gas outlet of a shake flask and flushed with gas from the flask headspace for 5 min to allow for saturation of the bag material with ET. Next, the gas bags were emptied by pulling vacuum with a syringe and then refilled for 10 min. The sample volume taken from the shake flask was approximately 110 mL. The gas chromatograph (GC) was a Thermo Fisher Trace GC Ultra (Thermo Scientific, Schwerte, Germany) using a PoraPlotQ column (30 m × 0.32 mm ID, Agilent Technologies, Santa Clara, California) and helium as carrier gas. As detector a Thermo Fisher ISQ mass spectrometer (MS) was used (Thermo Scientific, Schwerte, Germany). Calibration gas containing 1 ppm ET in air and a gas mixture (using aforementioned calibration gas) containing 0.1 ppm ET in air served as standards for GC-MS measurements. Every sample taken from a shake flask was analyzed three times.
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