Dcfh da fluorescence kit

Manufactured by Beyotime

Sourced in China

The DCFH-DA fluorescence kit is a laboratory tool used to measure the intracellular production of reactive oxygen species (ROS) in cells. It contains the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA), which can be oxidized by ROS to form the fluorescent compound 2',7'-dichlorofluorescein (DCF). The intensity of the DCF fluorescence is proportional to the level of intracellular ROS.

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Lab products found in correlation

Rhodamine phalloidin, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Abcam (1 mentions) Bcip nbt alp color development kit, by Beyotime (1 mentions) Alexa fluor 594 conjugated goat antirabbit igg secondary antibody, by Abcam (1 mentions)

Close spelling variants of this product

DCFH-DA (1253 citations) DCFH-DA kit (30 citations)

2 protocols using dcfh da fluorescence kit

Osteoblast Adhesion, Morphology, and Osteogenic Differentiation

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As described previously,^{44 (link)} osteoblast adhesion and morphology were assessed
by visualizing the intracellular actin filaments and nucleus following
staining with rhodamine phalloidin (Millipore Sigma, USA) and DAPI
(Millipore Sigma, USA), respectively. The osteogenic differentiation
potential was detected by ALP staining using BCIP/NBT ALP color development
kit (Beyotime Biotechnology, China) following incubation for 7 days
under osteogenic conditions. The ROS levels were determined by staining
the cells using a DCFH-DA fluorescence kit (Beyotime Biotechnology,
China). Immunofluorescence staining was carried out using primary
antibodies against NRF2 (1:200 dilution; Abcam, UK), an Alexa Fluor
594-conjugated goat antirabbit IgG secondary antibody (1:400; Abcam),
and DAPI for nuclear staining. All the above staining procedures were
carried out as per the manufacturers’ instructions, and the
images were visualized and captured using light (Olympus IX73, Olympus
Lifescience, Japan) or fluorescence (Leica, Germany) microscopes.

Zhang Y., Zhang J., Sun B., Ma L, & Ma Y. (2024). Catalpol Promotes Osseointegration of Titanium Implants under Conditions of Type 2 Diabetes via AKT/GSK3β/FYN Pathway-Mediated NRF2 Activation. ACS Omega, 9(5), 5761-5771.

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ROS Quantification in HBECs

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The intracellular ROS level was measured with a dichlorodihydro-fluorescein diacetate (DCFH-DA) fluorescence kit (Beyotime). HBECs were treated with NAC prior to 5% CSE stimulation and then loaded with DCFH-DA probes (10 μM) diluted in RPMI-1640 medium. Following incubation at 37°C for 20 mins, three washes with RPMI-1640 medium were performed. The generation of ROS was observed under a fluorescence microscope (Olympus) and quantified with the ImageJ software (version 1.46r).

Zhu M., Ye L., Zhu G., Zeng Y., Yang C., Cai H., Mo Y., Song X., Gao X., Peng W., Wang J, & Jin M. (2022). ROS-Responsive miR-150-5p Downregulation Contributes to Cigarette Smoke-Induced COPD via Targeting IRE1α. Oxidative Medicine and Cellular Longevity, 2022, 5695005.

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