The largest database of trusted experimental protocols

2 protocols using arabinocytosine

1

Molecular Mechanisms of Carnosic Acid

Check if the same lab product or an alternative is used in the 5 most similar protocols
Arabinocytosine and Doxercalciferol were purchased from Sigma-Aldrich (St. Louis, MO). Carnosic acid (CA) was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY). The following antibodies: Crk-L (sc-319), VDR (sc-1008), C/EBP beta (sc-150), and p27Kip1 (sc-528) were obtained from Santa Cruz Biotechnology (Dallas, TX). Phospho-Ser139-H2AX (#9718), phospho-Chk1 (#2348), and HRP-linked anti-rabbit (#7074) antibodies were purchased from Cell Signaling Technologies (Danvers, MA).
+ Open protocol
+ Expand
2

Caspase Inhibitors Enhance AraC Cytotoxicity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Arabinocytosine and Doxercalciferol (1α-hydroxyvitamin D2; 1-D2) were purchased from Sigma-Aldrich (St. Louis, MO). Carnosic acid (CA) was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY). The antibodies used for Western blots were: Bim (sc-8625), VDR (sc-1008), C/EBP beta (sc-150) and Crk-L (sc-319) were obtained from Santa Cruz Biotechnology (Dallas, TX). Phospho-Ser139-H2AX (#9718), phospho-Ser345-Chk1 (#2348), caspase 3, caspase 9 and HRP-linked anti-rabbit (#7074) antibodies were from Cell Signaling Technologies (Danvers, MA). The siRNA transfection reagents were from Santa Cruz Biotechnology (Dallas, TX), and Caspase 3 inhibitor (Z-DEVD-FMK) from Santa Cruz Biotechnology. Specific caspase 1 inhibitor (Z-WEHD-FMK) and negative control for caspase inhibitors (Z-FA-FMK) were from Abcam (Cambridge, MA). AML cells were pretreated with 100 nM AraC for 72 h, then incubated with 10 μM of caspase inhibitors for 1 h before adding other agents.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!