Anti tubulin antibody 2128 s

Anti-Flag (20543-1-AP; 1:1,000), anti-HA (51064-2-AP; 1:1,000), anti-MYC (16286-1-AP; 1:2,000) and anti-USP49 (18066-1-AP; 1:1,000) antibodies were purchased from Proteintech. Anti-FBXO45 antibody (orb156851; 1:1,000) was purchased from Biorbyt Company. Anti-tubulin antibody (2128 S; 1:1,000) was purchased from Cell Signaling Technology. Peroxidase-conjugated anti-mouse secondary antibody (70-GAM007, 1:5,000) and peroxidase-conjugated anti-rabbit secondary antibody (70-GAR0072; 1:5,000) were purchased from MultiSciences Company. Immunoprecipitation kit-HA tag immunomagnetic beads (TB100028, 50 μl for IP) and immunoprecipitation kit-DYKDDDDK (Flag) tag immunomagnetic beads (TB101274, 50 μl for IP) were purchased from Sino Biological Company.

Anti-FBXO31 antibody (#86137; 1:1000) antibody was purchased from Abcam. Anti-AuroraA antibody (#14475; 1:1,000), anti-Slug antibody (#9585; 1:1000), anti-C-myc antibody (#5605; 1:1000), anti-p-p44/42 MAPK (Erk1/2) (#4370; 1:1000) and anti-tubulin antibody (2128 S; 1:2000) antibodies were all obtained from Cell Signaling Technology. Anti-SIRT2 (66410-1-Ig; 1:1500), anti-METTL3 (15073-1-AP; 1:1500), anti-METTL14 (26158-1-Ap; 1:1500), Anti-Flag tag (20543-1-AP; 1:1500), anti-HA tag (51064-2-AP; 1:1000), Anti-β-actin (20536-1-AP; 1:2000) and anti-His tag (66005-1-Ig; 1:1,000) antibodies were all bought from Proteintech. Peroxidase-conjugated anti-mouse secondary antibody (70-GAM007, 1:5000) and peroxidase-conjugated anti-rabbit secondary antibody (70-GAR0072; 1:5,000) were purchased from MultiSciences Company.