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Potassium chloride (kcl)

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Potassium Chloride (KCl) is a common inorganic salt used in various laboratory applications. It is a white, crystalline solid that is highly soluble in water. KCl serves as an electrolyte and is commonly used to maintain proper ionic balance in cell culture media and other biological solutions.

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Lab products found in correlation

Sodium hydroxide (naoh), by BD (3 mentions) Sodium chloride (nacl), by BD (2 mentions) Acetylcholine, by BD (1 mentions) Norepinephrine, by BD (1 mentions) Fluvastatin, by Novartis (1 mentions) Acetylcholine iodide, by Merck Group (1 mentions) Potassium dihydrogen phosphate, by BD (1 mentions) Magnesium chloride, by BD (1 mentions) Calcium chloride, by BD (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Ethylene diamine tetraacetic acid (edta), by BD (1 mentions) Sulfuric acid, by BD (1 mentions) Acetonitrile, by BD (1 mentions) Chloroform, by BD (1 mentions) Potassium hydroxide, by BD (1 mentions) Xylene, by BD (1 mentions) Ferric chloride, by BD (1 mentions) Aflatoxin b1, by Merck Group (1 mentions) Congo red, by Merck Group (1 mentions) Crystal violet, by Merck Group (1 mentions)

7 protocols using potassium chloride (kcl)

1

Pharmacological Evaluation of Cardiovascular Agents

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Analytical-grade chemicals were used for the experiments. Acetylcholine (ACh) [15 (link)], Norepinephrine (NE), and Potassium chloride (KCL) were purchased from BDH, Poole, England. Atorvastatin raw material was purchased from Polyfine Pharmaceutical Private Ltd., Peshawar, Pakistan. Fluvastatin of Novartis Pharma was purchased from the local market of Peshawar. Amlodipine raw material was obtained from Feroz-sons laboratories Pvt Ltd., Nowshera, Pakistan.
Less soluble raw materials in Krebs solution were suspended in 0.01% carboxy methyl cellulose (CM) using distilled water. To rule out any possible effects of CMC, a negative control in distilled water was conducted. Freshly prepared solutions and suspensions were used on the same day of the experiments.
Ali N., Ali W., Ullah A., Ahmad S., Alsaiari A.A., Almehmadi M., Abdulaziz O., Allahyani M, & Aljuaid A. (2023). Atorvastatin and Fluvastatin Potentiate Blood Pressure Lowering Effect of Amlodipine through Vasorelaxant Phenomenon. Medicina, 59(6), 1023.
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2

Optimized Chemical Reagents for Biomolecular Analysis

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Adenosine triphosphate and acetylcholine iodide were obtained from Sigma Chemical Company. Chlorpyrifos was purchased from Nantong Jinling Agricultural Chemical Co., LTD. China. Sodium barbital, potassium dihydrogen phosphate, sodium chloride, Tris, EDTA, magnesium chloride, potassium chloride, calcium chloride, ammonium molybdate, aminonaphthol sulfonic acid (ANSA) were either obtained from BDH Ltd. Poole, England or Scharlab S.L. Spain. All reagents and chemicals used were of analytical grade.
Ajilore B.S., Alli A.A, & Oluwadairo T.O. (2018). Effects of magnesium chloride on in vitro cholinesterase and ATPase poisoning by organophosphate (chlorpyrifos). Pharmacology Research & Perspectives, 6(3), e00401.
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3

Aflatoxin Standards Characterization

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Aflatoxin B1, B2, G1 and G2 standards (crystalline powder) were purchased from Sigma–Aldrich (St. Louis–MO, USA). Pre–coated TLC plates of silica gel 60 (layer thickness 0.25 mm, 20 x 20 cm) on glass or aluminum, without fluorescent indicator were purchased from E. Merck (Darmstadt, Germany). Analytical grade acetone, acetonitrile, benzene, chloroform, cupric carbonate, ferric chloride, potassium chloride, potassium hydroxide, sodium hydroxide, sodium sulfate, sulfuric acid, xylene and other solvents procured from BDH (Poole, England).
ASGHAR M.A., IQBAL J., AHMED A, & KHAN M.A. (2014). Occurrence of Aflatoxins Contamination in Brown Rice from Pakistan. Iranian Journal of Public Health, 43(3), 291-299.
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4

Hydrogel Synthesis and Characterization

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3-Acrylamidopropyltrimethyl ammonium chloride (APTMACl, Sigma Aldrich, 75 wt%) and 2-acrylamido-2-methylpropanesulphonic acid (AAMPSA, 98%, Alfa Aesar) were used as cationic monomer and anionic monomer, respectively. N,N′-Methylenebisacrylamide (MBA, 99%, Sigma Aldrich) was used as a cross-linker, and ammonium persulfate (APS 98%, Sigma Aldrich) was used as an initiator. These chemicals were directly used for synthesizing hydrogels. Congo red (CR, Sigma Aldrich) and crystal violet (CV, Sigma Aldrich) as sources of organic dyes were used as-received. Milli-Q distilled water was used throughout the experimental work. NaNO3 (Merck), NaOH (BDH), HCl (Sigma), and KCl (BDH) were used as reagents during the research work.
Rehman T.U., Shah L.A., Khan M., Irfan M, & Khattak N.S. (2019). Zwitterionic superabsorbent polymer hydrogels for efficient and selective removal of organic dyes. RSC Advances, 9(32), 18565-18577.
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5

Synthesis of Calcium Phosphate Composites

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Analytical reagent grade anhydrous calcium nitrate (Ca(NO3)2, urea (NH2)2CO, sodium dihydrogen phosphate dihydrate (NaH2PO4.2H2O), NaOH, NaCl, KCl, CaCl2.2H2O, and Na2S.9H2O were purchased from BDH and Scharlau. Benzoyl peroxide (C14H10O4), Methyl-methacrylate-MMA (C5H8O2) and polymethyl-methacrylate-PMMA (C5H8O2)n were purchased from Scharlau. All the stock solutions were filtered through a micropore membrane to remove the insoluble impurities.
Akhtar K., Pervez C., Zubair N, & Khalid H. (2021). Calcium hydroxyapatite nanoparticles as a reinforcement filler in dental resin nanocomposite. Journal of Materials Science. Materials in Medicine, 32(10), 129.
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6

Colorimetric RT-LAMP Assay for SARS-CoV-2 Detection

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RT-LAMP reaction was performed in a total volume of 20 μL containing the following components: 8 U Bst 2.0 (NEB), 7.5 U RTx (NEB) and 1 × colorimetric buffer mix [1.6 μM FIP/BIP primers, 0.4 μM LF/LB primers, 0.2 μM F3/B3 primers Gene N-A24 (link), 10 mM (NH4)2SO4 (Merck), 50 mM KCl (BDH), 8 mM MgSO4 (BDH), 0.1% Tween 20, 0.2 mM Phenol Red (Sigma), 1.4 mM each dNTP (NZYTech)]. For the in-house-made assay, we used the same colorimetric buffer mix, 0.5 μL of MashUP RT (6.8 mg/mL) and 1 μL of Bst LF (7.6 mg/mL) 50 × diluted. WarmStart colorimetric LAMP 2 × master mix (M1800S, NEB) was also used with the above final primer concentration.
When the complexometric indicator MX-Zn was used, samples were assembled as described above, but without Phenol Red. After 30 min, 2 μL of 5 mM Murexide and 1 μL of 50 mM of ZnCl2 were added to the reaction, in a post-LAMP workspace. All reactions were performed in a thermocycler at 65 °C and pictures were taken at the indicated time points. Figures depicting the readout of the RT-LAMP assays are representative of three independent experiments.
Amaral C., Antunes W., Moe E., Duarte A.G., Lima L.M., Santos C., Gomes I.L., Afonso G.S., Vieira R., Teles H.S., Reis M.S., da Silva M.A., Henriques A.M., Fevereiro M., Ventura M.R., Serrano M, & Pimentel C. (2021). A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples. Scientific Reports, 11, 16430.
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7

Mitochondrial Metabolism and Cell Death Assays

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Mitochondrial metabolism assays were performed using 20 μg of isolated mitochondria were re-suspended in Locke's buffer (154 mM NaCl, 5.6 mM KCl (BDH), 2.3 mM CaCl2 (Fischer), 1 mM MgCl2, 3.6 mM NaHCO3, 5 mM Glucose and 5 mM HEPES pH 7.5 (BDH) and plated into 1 well of a black 96 well plate (CLS3904). 40 μM resazurin was then added to each sample to make a final concentration of 20 μM and plates were then incubated at 37 o C with 5% CO2. Absorbance was then read at 595nm every 30 minutes for 4 h and compared to that of 20 μM resazurin blank controls (FluoStar Galaxy).
Cell death curves were performed on 50,000 HeLa cells expressing GFP, pre-treated with and without 1 μM of the BCL-2 inhibitor Navitoclax for 16h. Cells were treated with 0, 0.02, 0.08, 0.32, 1.28 or 2.56 J of UV, left for 24 h, 20 μM of resazurin added and plates incubated at 37 o C with 5% CO2 for 1h. Absorbance was read at 595nm and compared to 20 μM resazurin control.
Townley A.R., & Wheatley S.P. (2020). Mitochondrial survivin reduces oxidative phosphorylation in cancer cells by inhibiting mitophagy.
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