Anti hmgb1 ab18256

Manufactured by Abcam

Sourced in United Kingdom

Anti-HMGB1 (ab18256) is a primary antibody that specifically binds to the High Mobility Group Box 1 (HMGB1) protein. HMGB1 is a nuclear protein involved in DNA binding and transcriptional regulation. This antibody can be used to detect and study the HMGB1 protein in various research applications.

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Lab products found in correlation

Chloroquine c6628, by Merck Group (1 mentions) Alexa fluor 488 goat anti rabbit igg a11034, by Thermo Fisher Scientific (1 mentions) Goat anti mouse 115 035 003, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit 111 035 003, by Jackson ImmunoResearch (1 mentions) Anti perk 5683, by Cell Signaling Technology (1 mentions) Anti lc3 antibody nb100 2220, by Novus Biologicals (1 mentions) Anti flag antibody f3165, by Merck Group (1 mentions) Losartan, by Merck Group (1 mentions) Anti gapdh sc 32233, by Santa Cruz Biotechnology (1 mentions) Caffeic acid phenethyl ester, by Merck Group (1 mentions) Ang 2, by Merck Group (1 mentions) Anti caspase 1 p20 ag 20b 0042 c100, by Adipogen (1 mentions) Tcs sp8, by Leica (1 mentions) Enhanced chemiluminescence solution, by Thermo Fisher Scientific (1 mentions) Cytotox 96 non radioactive cytotoxicity assay g1780, by Promega (1 mentions) Punicalagin p0023, by Merck Group (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Anti bid, by R&D Systems (1 mentions) Anti β actin hrp, by Santa Cruz Biotechnology (1 mentions) Pam3csk4 tlrl pms, by InvivoGen (1 mentions)

Close spelling variants of this product

HMGB1 (192 citations) Ab18256 (171 citations) Anti-HMGB1 (70 citations)

4 protocols using anti hmgb1 ab18256

Quantifying Autophagy Pathway Activation

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Chloroquine (C6628) was obtained from Sigma-Aldrich. Oxaliplatin (OXA, S1224), and quinacrine (S5435) were purchased from Selleckchem. Torin 1 (2273-5) was ordered from BioVision. Anti-ATF4 (CST 11815S), anti-CHOP antibody (2895), anti-eIF2α antibody (9722), anti-p-eIF2α (Ser51) (9721S), anti-Histone H3 (9715S), anti-ATG5 antibody (12994), and anti-PERK (5683) antibodies were obtained from Cell Signaling Technology (CST). An anti-LC3 antibody (NB100-2220) was obtained from Novus Biologicals. An anti-GAPDH antibody (GTX100118) was ordered from GeneTex. Anti-TFE3 antibody (HPA023881), and anti-Flag (F3165) antibody were ordered from Sigma-Aldrich. Anti-TFEB antibody (303-673A) was ordered from Bethyl Laboratories. Anti-HMGB1 (ab18256), and anti-Calreticulin (ab92516) antibodies were ordered from Abcam. Goat anti-rabbit (111-035-003), and goat anti-mouse (115-035-003) secondary antibodies were products of Jackson ImmunoResearch. Alexa Fluor 594 goat anti-rabbit IgG (A-11012), Alexa Fluor 488 goat anti-rabbit IgG (A-11034), and Alexa Fluor 488 (A-11008) goat anti-rabbit secondary antibodies were ordered from Invitrogen.

Wang S., Wang G., Wu W., Xu Z., Yang J., Cao M., Wang Q., Wang J., Yang C, & Zhang W. (2022). Autophagy activation by dietary piceatannol enhances the efficacy of immunogenic chemotherapy. Frontiers in Immunology, 13, 968686.

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RAGE-Fc Fusion Protein Modulation

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Purified human soluble RAGE-Fc fusion protein (sRAGE) was purchased from Y-biologics (Daejeon, Republic of Korea). Ang II, losartan, caffeic acid phenethyl ester (CAPE), and fluorescein isothiocyanate (FITC)-labeled dextran 40 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Purified anti-HMGB1 antibody (651402) was purchased from BioLegend (San Diego, CA, USA). Anti-AT1R antibody (AAR-011) was purchased from Alomone Labs (Hadassah Ein Kerem, Israel). Anti-RAGE (ab3611), anti-mDia1 (ab129167), and anti-HMGB1 (ab18256) antibodies were purchased from Abcam (Cambridge, UK). Anti-Src (2109s), anti-phospho-Src (6943s, Tyr416), anti-β-catenin (8480s), anti-phospho β-catenin (5651s, Ser522), and anti-phospho-NF-κB (3033s, Ser536) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-phospho-VE-cadherin (LS-C357312, Tyr731) was purchased from LifeSpan Biosciences (Seattle, WA, USA). Anti-VE-cadherin (sc9989), anti-NF-κB (sc8008), anti-β-actin (sc47778), and anti-GAPDH (sc32233) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Jeong J., Lee J., Lim J., Cho S., An S., Lee M., Yoon N., Seo M., Lim S, & Park S. (2019). Soluble RAGE attenuates AngII-induced endothelial hyperpermeability by disrupting HMGB1-mediated crosstalk between AT1R and RAGE. Experimental & Molecular Medicine, 51(9), 113.

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Immunofluorescence Imaging of Neuroinflammatory Markers

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The mice were anesthetized with 1.5% sodium pentobarbital and perfused transcardially with phosphate-buffered saline (PBS), followed by 4% paraformaldehyde (PFA). Their brains were dissected and fixed in 4% PFA for 24 h, and coronal cryosections (30 μm thick) were obtained from the experimental animals. Anti-tau Ser404 (ab92676) and anti-HMGB1 (ab18256) antibodies were purchased from Abcam (Cambridge, United Kingdom), and anti-NLRP3 (AG-20B-0014-C100), anti-ASC (AG-25B-0006-C100), and anti-caspase-1 (P20) (AG-20B-0042-C100) antibodies were purchased from AdipoGen Life Sciences (San Diego, CA, United States) (detailed information in Supplementary Table 1). Fluorescence images were acquired using a laser scanning confocal fluorescence microscope (TCS SP8; Leica, Wetzlar, Germany). The fluorescence images were converted to 8-bit images, and then the stained area was quantified using the ImageJ software. Detection of the background staining was eliminated by setting the same threshold to all the images.

Zhao Y., Tan S.W., Huang Z.Z., Shan F.B., Li P., Ning Y.L., Ye S.Y., Zhao Z.A., Du H., Xiong R.P., Yang N., Peng Y., Chen X, & Zhou Y.G. (2021). NLRP3 Inflammasome-Dependent Increases in High Mobility Group Box 1 Involved in the Cognitive Dysfunction Caused by Tau-Overexpression. Frontiers in Aging Neuroscience, 13, 721474.

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Purification and Characterization of Anthrax Toxin Components

Cited 1 time

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Recombinant expression and purification of LFn-FlaA was performed as previously described [19 (link)]. B. anthracis protective antigen (PA) and lethal factor (LF) were acquired from List Biologicals. TMRM (T668), Lysotracker (L7528), Sytox Green (S7020), Mitotracker (M7512), Fluo4 (F14217), Pluronic F-127 (P6867), Hoechst 33342 Trihydrochloride Trihydrate (H1399), PI (P3566) and CTB coupled to Alexa 594 (C22842) or 647 (C34778) were purchased from Thermo Scientific. PI solution (556463) and Annexin-V–FITC (556419) were from BD Biosciences. The antibodies used in the study were anti-Caspase-1 (AG-20B-0042-C10, Adipogen), anti-Cathepsin B (31718S, Cell Signaling Technology), anti-Cytochrome c (11940S, Cell Signaling Technology), anti-HMGB1 (ab18256, Abcam), anti-Caspase-3 (9662S, Cell Signaling Technology) and anti-β-Actin-HRP (sc-47778, Santa Cruz Biotechnology), anti-BID (AF860, R&D Systems). Horseradish peroxidase (HRP)-conjugated secondary antibodies were acquired from Jackson Immunoresearch Laboratories and enhanced chemiluminescence solution was from Thermo Scientific. Punicalagin (P0023) was from Sigma Aldrich and the CytoTox 96 Non-Radioactive Cytotoxicity Assay (G1780) and FugeneHD Transfection Reagent were purchased from Promega. Y27632 and (−)-blebbistatin were acquired from Selleckchem. Pam3csk4 (tlrl-pms) and LPS-SM (tlrl-smlps) were acquired from Invivogen.

de Vasconcelos N.M., Van Opdenbosch N., Van Gorp H., Parthoens E, & Lamkanfi M. (2018). Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture. Cell Death and Differentiation, 26(1), 146-161.

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