The largest database of trusted experimental protocols

Mcp 1 sirna

Manufactured by Santa Cruz Biotechnology

MCP-1 siRNA is a short interfering RNA (siRNA) designed to target the Monocyte Chemoattractant Protein-1 (MCP-1) gene. MCP-1 is a chemokine that plays a role in the recruitment and activation of monocytes and macrophages. The MCP-1 siRNA can be used to knockdown the expression of the MCP-1 gene in cell-based experiments.

Automatically generated - may contain errors

2 protocols using mcp 1 sirna

1

Biochemical Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
NaHS was purchased from Sigma-Aldrich. CBS, CSE, and MCP-1 siRNA were purchased from Santa Cruz Biotechnology.
+ Open protocol
+ Expand
2

Invasion Assay Procedure for Cell Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols
MTT assay kit was purchased from Roche Molecular Biochemicals (Indianapolis, IN). Transwells were purchased from Becton Dickinson Labware (Franklin lakes, NJ). Matrigel Invasion Chambers were purchased from BD Biosciences (Bedford, MA). Alcohol (200 Proof) was obtained from Fisher Scientific (Pittsburgh, PA). MCP-1 Human ELISA Kit was purchased from Invitrogen Corporation (Carlsbad, CA). Anti-human MCP-1 antibody and CCR2 antagonist (CCR2-I) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Recombinant Human MCP-1/CCL2 was purchased from Biolegend (San Diego, CA). Anti-β-catenin, anti-non phospho β-catenin (Ser33/37/Thr41), anti-phospho-GSK3β and anti-GSK3β antibodies were purchased from Cell Signaling Technology Inc. (Beverly, MA). β-catenin siRNA, MCP-1 siRNA and CCR2 siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-Ref-1 antibody was provided by Dr. Xianglin Shi (University of Kentucky, Lexington, KY). NE-PER Nuclear and Cytoplasmic Extraction Kit was purchased from Thermo Scientific (Rockford, IL). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!