Under the detection of a caspase-3 and -9 activity assay kit (Beyotime, Haimen, Jiangsu, China), VSMCs apoptosis was monitored. In short, VSMCs lysates undergoing different treatments were gathered through adding lysis buffer (ABGENT, Suzhou, Jiangsu, China). Thereafter, VSMCs lysates were supplemented with 2 mM of Asp-Glu-Val-Asp (DEAD, for caspase–3) and 2 mM Leu-Glu-His-Asp (LEHD, for caspase-9) labeled with p–nitroaniline (pNA) at 37°C for 2 h in the dark. Absorbance at 405 nm was measured by Labserv K3 microplate reader (WoYuan, Hongkou, Shanghai, China).
Lysis buffer
Manufactured by Abcepta
Sourced in China
Lysis buffer is a solution used to break open cells and extract their contents, including proteins, nucleic acids, and other biomolecules. It typically contains a combination of detergents, salts, and buffering agents that disrupt the cell membrane and facilitate the release of the desired cellular components.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lysis buffer
1
Caspase-3 and -9 Activity Assay
2
Trophoblast Cell Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Trophoblast cell apoptosis was evaluated using caspase-3 and caspase-9 activity assay kits (Beyotime). Briefly, the transfected trophoblast cell lysates were collected after the addition of lysis buffer (Abgent, Suzhou, Jiangsu, China). Cell lysates were treated with 2 mM Asp-Glu-Val-Asp (to assay caspase-3 activity) or 2 mM Leu-Glu-His-Asp (to assay caspase-9 activity), and labeled with p-nitroaniline for 2 h in the dark at 37°C. The OD value at 405 nm was measured using a Labserv K3 microplate reader (WoYuan, Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!