Ab74666

Manufactured by Abcam

Sourced in United Kingdom

Ab74666 is a laboratory equipment product manufactured by Abcam. It is designed for use in scientific research applications. The core function of this product is to provide a specific capability or measurement for researchers, but a detailed description is not available at this time while maintaining an unbiased and factual approach.

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Lab products found in correlation

E cadherin nch 38, by Agilent Technologies (2 mentions) Chromogranin a, by Immunostar (2 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Lysozyme, by Abcam (1 mentions) Alexa 596, by Thermo Fisher Scientific (1 mentions) M0851, by Agilent Technologies (1 mentions) M0760, by Agilent Technologies (1 mentions) Histogel, by StatLab (1 mentions) Ep17 ep30, by Agilent Technologies (1 mentions) β catenin 1, by Agilent Technologies (1 mentions) Ab92547, by Abcam (1 mentions)

2 protocols using ab74666

Immunofluorescence Staining of Cell Samples

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Cell samples were fixed with 4% paraformaldehyde for 15 min and permeabilized by 1% Triton X-100 for 10 min at RT. The cells were probed overnight at 4°C with relevant antibodies prepared in PBS (10 mM, pH 7.4): antibodies against Lysozyme (ab74666, Abcam, United Kingdom), Chromogranin A (20086, ImmunoStar, United States), Villin1 (1D2C3, Dako, Denmark), E-cadherin (NCH-38, Dako, Denmark), Sox9 (EPR14335, Abcam, United Kingdom), and monoclonal antibodies against PDCoV N proteins (2H7). Secondary antibodies were either Alexa 488- or Alexa 596-conjugated (ThermoFisher Scientific, United States). Nuclei were stained with DAPI (1 μg/mL). The stained cells were examined by the inverted fluorescence microscope X81.

Luo H., Zheng J., Chen Y., Wang T., Zhang Z., Shan Y., Xu J., Yue M., Fang W, & Li X. (2020). Utility Evaluation of Porcine Enteroids as PDCoV Infection Model in vitro. Frontiers in Microbiology, 11, 821.

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Histological Characterization of Intestinal Tissue

Cited 2 times

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Intestinal tissue was fixed overnight in 10% neutral buffered formalin and stored in 70% ethanol until further histologic processing. Intestinal cultures were fixed in 10% buffered formalin for 5 minutes then resuspended in 30 μL preheated Histogel (American MasterTech) and stored in ethanol as above. Specimens were embedded in paraffin and sectioned at 3 μm thickness. Hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) were performed per standard protocol (Lahar et al. 2011 (link); Jabaji et al. 2013 (link)). Staining was done using antibodies against CD10 (56C6, Dako, Carpinteria, CA), lysozyme (ab74666, Abcam, Cambridge, MA), chromogranin A (20086, Immunostar, Hudson, WI) (Gonzalez et al. 2013 (link)), villin (1D2C3, Dako), E-cadherin (NCH-38, Dako), β-catenin (β-Catenin-1, Dako), p120-catenin (98, Ventana, Tucson, AZ), and cytokeratin 8 and 18 (EP17/EP30, Dako). Goblet cells were identified with the periodic acid-Schiff (PAS) stain (Brown et al. 1988 (link)). Porcine ISEMFs were plated in 0.95 cm² culture wells, fixed as above, and stained with antibodies against α-smooth muscle actin (α-SMA, M0851, Dako), desmin (M0760, Dako), and vimentin (ab92547, Abcam). For immunofluorescent stains, conjugated goat secondary antibody (Invitrogen) was added at 1:200 dilution and nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen).

Khalil H.A., Lei N.Y., Brinkley G., Scott A., Wang J., Kar U.K., Jabaji Z.B., Lewis M., Martín M.G., Dunn J.C, & Stelzner M.G. (2016). A Novel Culture System for Adult Porcine Intestinal Crypts. Cell and tissue research, 365(1), 123-134.

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