Cyanobacteria were grown for 42 days in the same culture media described in Table 3 . The cultures were then exposed to UVA (using two Philips TL-K 40W/10R-UVA lamps providing 7.5 W/m2 of irradiance) and UVB lights (through two Philips TL 40W/12RS-UVB lamps providing 4.5 W/m2 of irradiance) for 72 h. To ensure efficient UV light exposure, the cultures were transferred to 250 mL clear optic polystyrene bottles with vented caps equipped with filters. MAA content was measured in irradiated cultures and compared with non-UV-exposed cultures that had been grown under cycles of fluorescent light for the same experimental period and in the same culture medium. Thus, the total experimental period for UV-exposed cultures was the same as those cultures which were not irradiated (both Brasilonema strains were grown for 87 days prior to the 72 h UV induction). The presence of MAAs was also inferred by absorbance of culture extracts between 310 to 360 nm.
Tl k 40w 10r uva lamps
Manufactured by Philips
The TL-K 40W/10R-UVA lamps are a type of fluorescent tube light produced by Philips. They are designed to emit ultraviolet-A (UVA) radiation, which is a specific range of the ultraviolet spectrum. The lamps have a power rating of 40 watts and a color rendering index (CRI) of 10R, indicating their reddish color appearance.
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Lab products found in correlation
2 protocols using tl k 40w 10r uva lamps
1
UV Induction of Mycosporine-like Amino Acids in Cyanobacteria
2
Inducing MAA Biosynthesis via UV and PAR Irradiation
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To induce MAA biosynthesis, cells were irradiated using a UVA source (maximum illumination at 370 nm) provided by twin Philips TL-K 40W/10-R UV-A lamps, and a UVB source (maximum illumination at 290 nm) provided by twin Philips TL 40W/12 RS SLV/25 lamps in addition to the PAR lamps (maximum illumination at 550 nm). Cell density were normalized to 0.4 mg dry weight mL culture -1
, and 30 mL of suspended cells were irradiated in 25 cm² tissue culture flasks with a hydrophobic filter cap (TP90026, TPP) at a distance of 40 cm with lamps at 24 ± 2°C under light / dark cycle (12 h : 12 h) for 3 days (Singh et al. 2008a) . Cultures were harvest at the beginning and at the end of the experiment, and MAAs were extracted for subsequent analysis of MAAs using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method described by Geraldes et al. (2020) . All the experiment was performed in triplicate.
, and 30 mL of suspended cells were irradiated in 25 cm² tissue culture flasks with a hydrophobic filter cap (TP90026, TPP) at a distance of 40 cm with lamps at 24 ± 2°C under light / dark cycle (12 h : 12 h) for 3 days (Singh et al. 2008a) . Cultures were harvest at the beginning and at the end of the experiment, and MAAs were extracted for subsequent analysis of MAAs using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method described by Geraldes et al. (2020) . All the experiment was performed in triplicate.
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