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Bond rx slide stainer

Manufactured by Leica camera
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The BOND RX slide stainer is a fully automated, random-access platform designed for immunohistochemistry (IHC) and in situ hybridization (ISH) staining of tissue sections on microscope slides. The system features programmable protocols, slide tracking, and efficient processing of multiple sample types simultaneously.

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Lab products found in correlation

Bond polymer refine detection, by Leica camera (1 mentions) Leica bond detection kit, by Leica camera (1 mentions) Bond epitope retrieval solution 1, by Leica camera (1 mentions)

3 protocols using bond rx slide stainer

1

Tissue Microarray Immunostaining Protocol

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The tissue microarray blocks were cut into 4-µm sections. Paraffin was removed with xylene, and antigens were retrieved by BOND epitope retrieval solution 2 (EDTA buffer, pH 9.0) performed on the Leica BOND RX slide stainer for 30 min at 100 °C. Tissue sections were incubated with NNMT-specific polyclonal antibodies (HPA059180, 1:200, Atlas Antibodies, Sigma) for 30 min. They were followed by visualization with the Leica Bond detection kit (DS9800).
Ogawa M., Tanaka A., Namba K., Shia J., Wang J.Y, & Roehrl M.H. (2022). Tumor stromal nicotinamide N-methyltransferase overexpression as a prognostic biomarker for poor clinical outcome in early-stage colorectal cancer. Scientific Reports, 12, 2767.
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2

Tissue Microarray Immunohistochemistry for SLC7A5

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The tissue microarray blocks were cut into 4-μm sections. Paraffin was removed with xylene, and antigens were retrieved by a BOND epitope retrieval solution 2 (EDTA buffer, pH 9.0) performed on the Leica BOND RX slide stainer for 40 min at 100°C. Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min. They were followed by visualization with the Bond Polymer Refine Detection (DS9800, Leica).
Ogawa M., Tanaka A., Maekawa M., Namba K., Otani Y., Shia J., Wang J.Y, & Roehrl M.H. (2024). Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer. PLOS ONE, 19(5), e0298362.
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3

Immunohistochemical Analysis of GALNT6

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The tissue microarray blocks were cut into 4-µm sections. Paraffin was removed with xylene, and antigens were retrieved using BOND epitope retrieval solution 1 (citrate buffer, pH 6.0; Leica) on a Leica BOND RX slide stainer for 30 min at 100 °C. Tissue sections were incubated with GALNT6-specific polyclonal antibodies (HPA011762, 1:150, Atlas Antibodies, Sigma) for 30 min, followed by visualization with the Leica Bond detection kit.
Ogawa M., Tanaka A., Namba K., Shia J., Wang J.Y, & Roehrl M.H. (2022). Early-Stage Loss of GALNT6 Predicts Poor Clinical Outcome in Colorectal Cancer. Frontiers in Oncology, 12, 802548.
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