DNA isolation was performed from frozen dry pellets of each cell line, using the DNAeasy Blood & Tissue kit (Qiagen). To carry out the ARN isolation, cells were stored in RNAeasy Lysis Buffer (Qiagen) plus 1% β-Mercaptoethanol (Sigma Aldrich), then the kit RNAeasy Plus (Qiagen) was used to isolate RNA, according to the manufacturer’s instructions.
Rnaeasy lysis buffer
Manufactured by Qiagen
Sourced in Germany
The RNAeasy Lysis Buffer is a reagent used in RNA extraction and purification processes. It is designed to efficiently lyse cells and tissues, releasing the cellular RNA for subsequent purification steps. The buffer contains a proprietary formula that facilitates the denaturation of proteins and the inactivation of RNases, ensuring the integrity of the extracted RNA.
Automatically generated - may contain errors
2 protocols using rnaeasy lysis buffer
1
Nucleic Acid Extraction from Cell Lines
2
Isolation and Quantification of Liver RNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RNA was isolated and prepared as described in detail in19 (link). In brief, pieces of 50 mg of liver tissue were homogenized in RNAeasy lysis buffer (Qiagen, Hilden, Germany) with 1% 2-mercaptoethanol. Total RNA was then isolated according to the manufacturer’s protocol (RNeasy, Qiagen, Hilden, Germany).
RNA concentrations and purity have been determined using a NanoDrop ND-1000 UV–Vis Spectrophotometer (Thermo Scientific, Karlsruhe, Germany) at 260 nm and 260/280 nm ratio, respectively. All samples were stored at −80 °C before further analysis by RNA-Seq.
RNA concentrations and purity have been determined using a NanoDrop ND-1000 UV–Vis Spectrophotometer (Thermo Scientific, Karlsruhe, Germany) at 260 nm and 260/280 nm ratio, respectively. All samples were stored at −80 °C before further analysis by RNA-Seq.
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